Promutagen activation by Vicia faba: An assay based on the induction of sister-chromatid exchanges in Chinese hamster ovary cells

Takehisa, Shin; Kanaya, Nobuhiro; Rieger, R.

doi:10.1016/0027-5107(88)90093-0

Cited by 24 publications

(4 citation statements)

References 25 publications

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“…The roots were macerated and homogenised at 4 °C in 0.1 M sodium-phosphate buffer, pH 7.4. The ratio of the buffer solution in millilitres (2.0-2.5) to the fresh weight of root cuttings in grams (2.0-2.5) was 1:1 (26). Homogenised roots were centrifuged for 15 min at 10000 x g at 4 °C.…”

Section: Treatments With Metabolic Activationmentioning

confidence: 99%

“…In case of plant exposure, Vicia faba is considered to be a good plant system for mutagenic studies since it can metabolise the studied pesticide and form metabolically active compounds. As such, it is a sensitive and effective plant to study the effects of pesticide metabolites on genome stability measured by the SCE assay either in the plant or on other cell lines with promutagen extracts (25)(26)(27).…”

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confidence: 99%

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Assessment of genotoxicity of Lannate-90® and its plant and animal metabolites in human lymphocyte cultures

Valencia-Quintana

Gómez‐Arroyo

Sánchez-Alarcón

et al. 2016

Archives of Industrial Hygiene and Toxicology

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This study evaluated direct and metabolic genotoxic effects caused by Lannate-90 ® , a methomyl-based formulation (90 % active ingredient), in human lymphocyte cultures using sister chromatid exchange assay (SCE). Two processes were used for the plant promutagens evaluation: in vivo activation, applying the insecticide systemically in plants for 4 h and subsequently adding plant metabolites containing extracts to lymphocyte cultures; and in vitro activation, where the insecticide was incubated with Vicia faba S10 mix plus human lymphocyte culture. Direct treatment with the insecticide significantly increased SCE frequency in human lymphocytes (250-750 mg L ® to treat human lymphocytes, a dose-response relationship was observed. In lymphocyte cultures treated directly with the insecticide for 2 h, a negative response was obtained. When S10 mix was added, SCE frequency did not change significantly. Meanwhile, a mixture of S9 mammalian metabolic mix and Lannate-90 ® increased the SCE frequency, with an observed concentration-dependent response. Although Lannate-90 ® induced cellular death at the highest concentrations, it did not cause a delay in cell proliferation in any of the treatments, confirming its genotoxic action. This study is one of the first to evaluate and compare the direct effect of Lannate-90 ® in two bioassays, animal and vegetal, and the effect of plant and animal metabolism on its genotoxic potential.

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Section: Treatments With Metabolic Activationmentioning

confidence: 99%

mentioning

confidence: 99%

Assessment of genotoxicity of Lannate-90® and its plant and animal metabolites in human lymphocyte cultures

Valencia-Quintana

Gómez‐Arroyo

Sánchez-Alarcón

et al. 2016

Archives of Industrial Hygiene and Toxicology

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show abstract

“…In case of plant exposure, Vicia faba is considered a good plant system for treatment since it is metabolically active and contains the S10 enzymatic fraction. As such, it is a sensitive and effective plant to study the effects of pesticide metabolites on the genome stability using the SCE assay, in the plant itself, or on other cell lines with promutagen extracts from the treated Vicia faba roots (23,24).…”

Section: Abstract: Cellular Proliferation Kinetics; Plant and Animalmentioning

confidence: 99%

Genotoxic effects of the carbamate insecticide Pirimor-50® in Vicia faba root tip meristems and human lymphocyte culture after direct application and treatment with its metabolic extracts

Valencia-Quintana

Gómez‐Arroyo

Sánchez-Alarcón

et al. 2016

Archives of Industrial Hygiene and Toxicology

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The aim of the study was to evaluate genotoxic effects of Pirimor-50 ® , a pirimicarb-based formulation (50 % active ingredient), in human lymphocyte cultures and Vicia faba root meristems. Furthermore, the objective was to examine a combined influence of insecticide treatment with mammalian microsomal S9 and vegetal S10 metabolic fractions or S10 mix metabolic transformation extracts (after Vicia faba primary roots treatment with Pirimor-50 ® ). We used sister chromatid exchange assay-SCE and measured cell cycle progression and proliferation (proportion of M 1 -M 3 metaphases and replication index ratio-RI). Two processes were used for plant promutagen activation: in vivo activation-Pirimor-50 ® was applied for 4 h to the plant and then S10 mix was added to lymphocytes; and, in vitro activation-lymphocytes were treated with Pirimor-50 ® and S10 or S9 for 2 h. Direct treatment induced significantly higher SCE frequencies in meristems at 0.01 mg mL -1 . In lymphocytes, significantly higher SCE was at 1 mg mL -1 with decrease in RI and M 1 -M 3 metaphase proportions at 0.5 mg mL -1 and cell division stop at 2.5 mg mL 1 . S10 mix lymphocyte treatment showed significantly elevated SCE values at 2-2.5 mg mL -1 , with cell death at 3 mg mL -1. Lymphocyte treatment with Pirimor-50 ® together with S9 or S10 showed slightly elevated SCE frequency but had a significant influence on RI decrease, with lowest values in S9 treatment. Since no data are available on the genotoxicity of Pirimor-50 ® , this study is one of the first to evaluate and compare its direct effect in two bioassays, animal and vegetal, and also the effect of plant and animal metabolism on its genotoxic potential.

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“…MH (l,2-dihydropyridazine-3,6-dione) a promutagen [3][4][5]; since its discovery has been of growing importance as an herbicide with uses including inhibition of sprouting in vegetables and stored root crops, prevention of sucker production in tobacco plants and growth control of grass, and foliage [6]. o-PDA and m-PDA are promutagenic monocyclic arylamines/aromatic amine used in the preparation of various polymers, as an important constituent of hair dyes and dyes for leather and textiles [7].…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of promutagens o-PDA, m-PDA and MH for genotoxic response in root cells of Allium cepa L.

Ghosh

Paul

Sinha

et al. 2010

Nucleus

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A wide number of chemicals from different sources are released into the environment, some of which upon metabolic activation are converted to mutagenic products. These chemicals might hence possess threat to public health. Genotoxic potential of three promutagens -o-phenylenediamine (o-PDA), mphenylenediamine (m-PDA) and maleic hydrazide (MH) was evaluated using Allium cepa as the test system. Following treatment the genotoxic endpoints measured in the root tissue were mitotic index, cells with mitotic or chromosome aberration and micronuclei. DNA damage in root cells was evaluated by comet assay. EC 50 determined on the basis of root growth for o-PDA, m-PDA, MH were between 7-8 mg/l, 65-70 mg/l and 9-10 mg/l respectively. All the three agents induced genotoxicity significantly, that followed a dose response. While o-PDA and m-PDA did not induce DNA damage significantly, induction of DNA damage by MH was significant and dose dependent. The study identifies the genotoxic potential and re-establishes the fact that Allium cepa could ideally be used for evaluation of chromosome aberration and DNA damage.

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Promutagen activation by Vicia faba: An assay based on the induction of sister-chromatid exchanges in Chinese hamster ovary cells

Cited by 24 publications

References 25 publications

Assessment of genotoxicity of Lannate-90® and its plant and animal metabolites in human lymphocyte cultures

Assessment of genotoxicity of Lannate-90® and its plant and animal metabolites in human lymphocyte cultures

Genotoxic effects of the carbamate insecticide Pirimor-50® in Vicia faba root tip meristems and human lymphocyte culture after direct application and treatment with its metabolic extracts

Comparative evaluation of promutagens o-PDA, m-PDA and MH for genotoxic response in root cells of Allium cepa L.

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