Promutagen activation of triazine herbicides metribuzin and ametryn through Vicia faba metabolism inducing sister chromatid exchanges in human lymphocytes in vitro and in V. faba root tip meristems

Flores-Maya, Saúl; Gómez‐Arroyo, Sandra; Calderón-Segura, María Elena; Villalobos‐Pietrini, Rafael; Waliszewski, Stefan M.; Cruz, Lara Michelly Carvalho

doi:10.1016/j.tiv.2004.09.002

Cited by 17 publications

(10 citation statements)

References 45 publications

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“…The positive control roots were exposed to 3600 mg L -1 of ethanol (0.1 M) for 4 h at 20 °C. Ethanol is a promutagen in Vicia faba and increases the SCE frequency (27,(33)(34)(35). The negative control was handled under the same experimental conditions, but the roots of Vicia faba were immersed in distilled water alone to obtain the S10 fraction.…”

Section: Treatments With Metabolic Activationmentioning

confidence: 99%

“…The supernatant was sterilised using Millipore filters (0.45 μm pore size) and immediately used to treat human lymphocytes in culture. The total protein concentration in these extracts was determined using the Bio-Rad method (35). Protein concentration was fairly constant from one experiment to the other, with values between 5.0-5.9 μg μL -1 .…”

Section: Treatments With Metabolic Activationmentioning

confidence: 99%

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Assessment of genotoxicity of Lannate-90® and its plant and animal metabolites in human lymphocyte cultures

Valencia-Quintana

Gómez‐Arroyo

Sánchez-Alarcón

et al. 2016

Archives of Industrial Hygiene and Toxicology

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This study evaluated direct and metabolic genotoxic effects caused by Lannate-90 ® , a methomyl-based formulation (90 % active ingredient), in human lymphocyte cultures using sister chromatid exchange assay (SCE). Two processes were used for the plant promutagens evaluation: in vivo activation, applying the insecticide systemically in plants for 4 h and subsequently adding plant metabolites containing extracts to lymphocyte cultures; and in vitro activation, where the insecticide was incubated with Vicia faba S10 mix plus human lymphocyte culture. Direct treatment with the insecticide significantly increased SCE frequency in human lymphocytes (250-750 mg L ® to treat human lymphocytes, a dose-response relationship was observed. In lymphocyte cultures treated directly with the insecticide for 2 h, a negative response was obtained. When S10 mix was added, SCE frequency did not change significantly. Meanwhile, a mixture of S9 mammalian metabolic mix and Lannate-90 ® increased the SCE frequency, with an observed concentration-dependent response. Although Lannate-90 ® induced cellular death at the highest concentrations, it did not cause a delay in cell proliferation in any of the treatments, confirming its genotoxic action. This study is one of the first to evaluate and compare the direct effect of Lannate-90 ® in two bioassays, animal and vegetal, and the effect of plant and animal metabolism on its genotoxic potential.

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Section: Treatments With Metabolic Activationmentioning

confidence: 99%

Section: Treatments With Metabolic Activationmentioning

confidence: 99%

Assessment of genotoxicity of Lannate-90® and its plant and animal metabolites in human lymphocyte cultures

Valencia-Quintana

Gómez‐Arroyo

Sánchez-Alarcón

et al. 2016

Archives of Industrial Hygiene and Toxicology

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“…Since major pesticides' commercial formulation showed more toxic effects than their active principles, it is important to examine the effect of the formulation on the metabolism in living organisms and to compare the results with the active compound (26). This is even more so since one of the formulations, Pirimor G, showed similar cell viability in three different cell line types, with no effect on the activation of different apoptotic or necrotic pathways, while the active ingredient showed significantly elevated levels of enzymes involved in these pathways (26).…”

Section: Abstract: Cellular Proliferation Kinetics; Plant and Animalmentioning

confidence: 99%

“…Aficida ® and Patton Flow ® , have been found capable of inducing genotoxic damage (17-18, 21, 25) in water living organisms, but no data is available on the genotoxicity of Pirimor-50 ® as a pirimicarbbased formulation in human lymphocytes and Vicia faba. In the only one study with Pirimor G formulation, Mesnage et al (26) studied the cytotoxic effects on three different types of human cell lines, HepG2, HEK293, and JEG3. They discovered that the formulation does not produce the same cytotoxic effect as the active ingredient alone (almost no difference in cell viability and no activation of Adenylate kinase activity or Caspases 3/7 activity).…”

Section: Pirimor-50mentioning

confidence: 99%

Genotoxic effects of the carbamate insecticide Pirimor-50® in Vicia faba root tip meristems and human lymphocyte culture after direct application and treatment with its metabolic extracts

Valencia-Quintana

Gómez‐Arroyo

Sánchez-Alarcón

et al. 2016

Archives of Industrial Hygiene and Toxicology

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The aim of the study was to evaluate genotoxic effects of Pirimor-50 ® , a pirimicarb-based formulation (50 % active ingredient), in human lymphocyte cultures and Vicia faba root meristems. Furthermore, the objective was to examine a combined influence of insecticide treatment with mammalian microsomal S9 and vegetal S10 metabolic fractions or S10 mix metabolic transformation extracts (after Vicia faba primary roots treatment with Pirimor-50 ® ). We used sister chromatid exchange assay-SCE and measured cell cycle progression and proliferation (proportion of M 1 -M 3 metaphases and replication index ratio-RI). Two processes were used for plant promutagen activation: in vivo activation-Pirimor-50 ® was applied for 4 h to the plant and then S10 mix was added to lymphocytes; and, in vitro activation-lymphocytes were treated with Pirimor-50 ® and S10 or S9 for 2 h. Direct treatment induced significantly higher SCE frequencies in meristems at 0.01 mg mL -1 . In lymphocytes, significantly higher SCE was at 1 mg mL -1 with decrease in RI and M 1 -M 3 metaphase proportions at 0.5 mg mL -1 and cell division stop at 2.5 mg mL 1 . S10 mix lymphocyte treatment showed significantly elevated SCE values at 2-2.5 mg mL -1 , with cell death at 3 mg mL -1. Lymphocyte treatment with Pirimor-50 ® together with S9 or S10 showed slightly elevated SCE frequency but had a significant influence on RI decrease, with lowest values in S9 treatment. Since no data are available on the genotoxicity of Pirimor-50 ® , this study is one of the first to evaluate and compare its direct effect in two bioassays, animal and vegetal, and also the effect of plant and animal metabolism on its genotoxic potential.

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“…Обнаружено, что фосфорорганические пестициды (хло-рофос, карбофос) также способны попадать через кор-невую систему и накапливаться в растениях картофеля, даже если их применяли в рекомендуемых дозах (Найн-штейн, 1973). Метаболизм многих ксенобиотиков, в том числе и пестицидов, в растениях может сопровождаться образованием более генотоксичных метаболитов, причем некоторые соединения активируются именно S9-фрак-цией растений, но не микросомными монооксигеназами млекопитающих (Plewa and Wagner, 1993;Gentile et al, 1982;Flores-Maya, 2005). Таким образом, для реальной оценки генетической безопасности исследованных нами пестицидов необходимо также изучение роли фермент-ных систем биотрансформации растений в модуляции ге-нотоксического потенциала данных соединений.…”

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Evaluation of mutagenic activity of the pesticides: actara, sencor, mospilan, pencozeb, fastac in the Ames test

Karamova

Denisova

Stasevski

2008

Ecological genetics

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1 Казанский государственный университет, Казань 2 Татарский научно-исследовательский институт сельского хозяйства, Казань мутагенная активность пяти пестицидов; актара, зенкор, моспилан, пенкоцеб, фастак, наиболее часто используемых для обработки посадок картофеля в Республике татарстан, была исследована в тесте Эймса. Предварительная оценка токсического действия исследованных пестицидов на тестерный штамм Salmonella typhimurium Ta 100 позволила нам выбрать оптимальные концентрации препаратов для исследования их мутагенной активности в тесте Эймса. Пестициды пенкоцеб, актара, моспилан и фастак ни в одной из исследованных концентраций не обладали мутагенным эффектом в тесте Эймса без метаболичсекой активации in vitro. Гербицид зенкор в концентрации 1 мкг/чашка индуцировал более чем двукратное превышение числа колоний ревертантов His + над спонтанным фоном мутирования тестерного штамма, что свидетельствует о его слабой мутагенной активности. метаболическая активация in vitro фракцией S9 печени крыс снижала мутагенный потенциал гербицида зенкор и практически не влияла на мутагенную активность пестицидов пенкоцеб, актара, моспилан, фастак.Key words: пестициды, генотоксичность, мутагенная активность, тест Эймса, метаболическая активация in vitro оценка Мутагенной активности пестицидов: актара, зенкор, Моспилан, пенкоцеб, фастак в тесте эйМса ВВедение Масштабное использование химических средств защиты растений яв-ляется одним из важнейших факторов интенсификации современного сель-скохозяйственного производства. В настоящее время активное применение находят свыше 600 видов пестицидов, для синтеза которых используются более 1500 видов химических соединений (Wilkinson, 1990). Способность к длительному сохранению в окружающей среде, обусловленная относительно высокой стабильностью, и выраженная биологическая активность пестици-дов позволяют отнести их к числу наиболее опасных загрязнителей окружа-ющей среды. Вышесказанное определяет необходимость строгого регламен-тирования применения химических средств защиты растений, основанного на результатах всестороннего исследования биологических эффектов данных соединений. Особого приоритета заслуживает оценка генотоксического по-тенциала препаратов как первичного фактора риска для долгосрочных пато-логических эффектов, таких, как канцерогенез и наследственные заболева-ния. На сегодняшний день большинство известных пестицидов тестированы на мутагенную активность (Dearfield, 1999;Bolognesi, 2003), тем не менее реальная оценка генотоксического потенциала многих пестицидов весьма затруднена из-за недостатка или противоречивости данных.В настоящей работе представлены результаты оценки мутагенного потен-циала пяти пестицидов: актара, зенкор, моспилан, пенкоцеб, фастак, наиболее часто используемых для обработки посадок картофеля в Республике Татарстан. Материалы и МетодыИсследованные соединения. В работе исследовано 5 пестицидов. Зенкор (метрибузин). Действующее вещество -4-амино-6-трет-бу-тил-3-(метилтио)-1,2,4-триазинон-5. Гербицид, действующий через листья и почву, уничтожая двудольн...

show abstract

Promutagen activation of triazine herbicides metribuzin and ametryn through Vicia faba metabolism inducing sister chromatid exchanges in human lymphocytes in vitro and in V. faba root tip meristems

Cited by 17 publications

References 45 publications

Assessment of genotoxicity of Lannate-90® and its plant and animal metabolites in human lymphocyte cultures

Assessment of genotoxicity of Lannate-90® and its plant and animal metabolites in human lymphocyte cultures

Genotoxic effects of the carbamate insecticide Pirimor-50® in Vicia faba root tip meristems and human lymphocyte culture after direct application and treatment with its metabolic extracts

Evaluation of mutagenic activity of the pesticides: actara, sencor, mospilan, pencozeb, fastac in the Ames test

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