Prooxidant effects of β-carotene in cultured cells

Palozza, Paola; Serini, Simona; Nicuolo, Fiorella Di; Piccioni, Elisabetta; Calviello, Gabriella

doi:10.1016/s0098-2997(03)00031-1

Cited by 132 publications

(77 citation statements)

References 58 publications

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“…This may also explain the negative effect of increasing b-carotene intakes in mth4 where some intakes were very high. To date a prooxidant effect has only been reported in in-vivo studies when high levels of dietary supplements are taken or shown in in-vitro studies of cell function (Omenn et al, 1996;Palozza et al, 2003). Mathews et al (2004) found high serum retinol levels in late pregnancy were associated with lower birthweight (Po0.001).…”

Section: Discussionmentioning

confidence: 99%

The association of maternal diet and dietary supplement intake in pregnant New Zealand women with infant birthweight

Watson

McDonald

2009

Eur J Clin Nutr

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Objective: To investigate the association of infant birthweight with maternal diet and supplement intake. Subjects/Methods: Prospective cohort study of 504 European and Polynesian urban and rural pregnant volunteers recruited from northern New Zealand clinics. Subjects were visited in months 4 (mth4) and 7 (mth7) of pregnancy when height, weight and skinfolds were measured, questionnaires to determine personal details administered, and diet assessed by a 24-hour recall and 3-day food record. Results: After adjusting for confounders nutrients accounted for up to 5.0% of the total variance in birthweight. Ethnicity was not a significant confounder. A quadratic relationship existed between birthweight and % total energy (%TE) from carbohydrate, fat and protein, most significantly with carbohydrate energy (P ¼ 0.002). Birthweight was greatest (B3600 g) when carbohydrate %TE was 48%, fat 35% and protein 17%. Birthweight was reduced with high b-carotene intakes (mth4, P ¼ 0.009) and with both high retinol and b-carotene intakes in mth4 and 7 (average). Birthweight was positively associated with increasing pantothenic acid/biotin ratios (P ¼ 0.011), magnesium (P ¼ 0.000) and vitamin D (P ¼ 0.015) intakes in mth4; with biotin (P ¼ 0.040) and B 12 intakes above the RDI (P ¼ 0.006) in mth7; and with pantothenic acid intake in mth4&7 (P ¼ 0.002). Dietary supplement usage was associated with increased birthweight, most significantly iron supplementation (P ¼ 0.006). Conclusion: Birthweight was associated with the %TE from carbohydrate, fat and protein, and with b-carotene, retinol, vitamins D and B 12 , pantothenic acid, biotin and magnesium intakes and iron supplementation. More research may be required on some dietary recommendations for pregnancy.

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Section: Discussionmentioning

confidence: 99%

The association of maternal diet and dietary supplement intake in pregnant New Zealand women with infant birthweight

Watson

McDonald

2009

Eur J Clin Nutr

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“…Moreover, an in vitro study with MDA-MB-231 cells found that doxorubicin inhibition of cell proliferation was increased by treatment with another fish oil-associated LC-PUFA, docosahexaenoic acid and that the affect was abolished by α-tocopherol (23). Despite these studies, it has been shown that some antioxidants such as vitamin E and β carotene may be able to function as prooxidants under certain conditions (24,25) and α-tocotrienol may therefore be oxidizing α-ESA. Thus, although these studies support our findings that α-ESA action may be dependent on an oxidation mechanism, it Y-axis, cell proliferation as a percent.…”

Section: Discussionmentioning

confidence: 99%

Eleostearic Acid Inhibits Breast Cancer Proliferation by Means of an Oxidation-Dependent Mechanism

Großmann

Mizuno

Dammen

et al. 2009

Cancer Prevention Research

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Eleostearic acid (α-ESA) is a conjugated linolenic acid that makes up ∼60% of Momordica charantia (bitter melon) seed oil. Prior work found that water extract from bitter melon was able to inhibit breast cancer. Here, we investigated effects of α-ESA on both estrogen receptor (ER)-negative MDA-MB-231 (MDA-wt) and ER-positive MDA-ERα7 human breast cancer cells. We found that α-ESA inhibited proliferation of both MDA-wt and MDA-ERα7 cells, whereas conjugated linoleic acid had comparatively weak antiproliferative activity at 20 to 80 μmol/L concentrations. We also found that α-ESA (40 μmol/L) treatment led to apoptosis in the range of 70% to 90% for both cell lines, whereas conjugated linoleic acid (40 μmol/L) resulted in only 5% to 10% apoptosis, similar to results for control untreated cells. Addition of α-ESA also caused loss of mitochondrial membrane potential and translocation of apoptosis-inducing factor as well as endonuclease G from the mitochondria to the nucleus. Additionally, α-ESA caused a G 2 -M block in the cell cycle. We also investigated the potential for lipid peroxidation to play a role in the inhibitory action of α-ESA. We found that when the breast cancer cells were treated with α-ESA in the presence of the antioxidant α-tocotrienol (20 μmol/L), the growth inhibition and apoptosis effects of α-ESA were lost. An AMP-activated protein kinase inhibitor (Dorsomorphin) was also able to partially abrogate the effects of α-ESA, whereas a caspase inhibitor (BOC-D-FMK) did not. These results illustrate that α-ESA can block breast cancer cell proliferation and induce apoptosis through a mechanism that may be oxidation dependent.

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“…It could, therefore, play a role in the control of inflammation and immune response through its antioxidant properties. However, recent research has shown that high-dose carotenoid supplementation may lead to both antioxidant and pro-oxidant reactions [68], depending on the redox potential of the biological environment in which it acts [69].…”

Section: Antioxidant Nutrientsmentioning

confidence: 99%

Air pollution, oxidative stress and dietary supplementation: a review

Romieu

Castro-Giner²,

Künzli³

et al. 2007

Eur Respir J

223

157

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The aim of the present review was to provide an up-to-date overview of the biological and epidemiological evidence of the role of oxidative stress as a major underlying feature of the toxic effect of air pollutants, and the potential role of dietary supplementation in enhancing antioxidant defences.A bibliographic search was conducted through PubMed. The keywords used in the search were ''air pollutant'', ''oxidative stress'', ''inflammation'', ''antioxidant polyunsaturated fatty acids'' and ''genetics''. In addition, the authors also searched for biomarkers of oxidative stress and nutrients.The review presents the most recent data on: the biological and epidemiological evidence of the oxidative stress response to air pollutants; the role of dietary supplementation as a modulator of these effects; and factors of inter-individual variation in human response. The methodology for further epidemiological studies will be discussed in order to improve the current understanding on how nutritional factors may act.There is substantial evidence that air pollution exposure results in increased oxidative stress and that dietary supplementation may play a modulating role on the acute effect of air pollutants. Further epidemiological studies should address the impact of supplementation strategies in the prevention of air-pollution-related long-term effects in areas where people are destined to be exposed for the distant future.

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Prooxidant effects of β-carotene in cultured cells

Cited by 132 publications

References 58 publications

The association of maternal diet and dietary supplement intake in pregnant New Zealand women with infant birthweight

The association of maternal diet and dietary supplement intake in pregnant New Zealand women with infant birthweight

Eleostearic Acid Inhibits Breast Cancer Proliferation by Means of an Oxidation-Dependent Mechanism

Air pollution, oxidative stress and dietary supplementation: a review

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