Propagação in vitro da farinha-seca

Rossi, Ediana; Sartoretto, Laudete Maria

doi:10.4336/2013.pfb.33.73.361

Cited by 6 publications

(3 citation statements)

References 11 publications

(11 reference statements)

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“…Auxins play a signi cant role in promoting various growth processes in plants such as root induction, cell division, differentiation, and elongation, in addition to their well-known function of promoting dominance [28,29]. Given the potent auxin effects of NAA and 2-4-D, it was expected that these treatments would result in high callus induction rates and growth.…”

Section: Discussionmentioning

confidence: 99%

Modeling and optimizing plant growth regulators for in vitro culture and antioxidant activity of Thymus daenensis

samadi

2024

Preprint

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Denaian thyme, also known as Thymus daenensis (Lamiaceae), is an endangered and endemic medicinal plant in Iran. It is commonly utilized in traditional remedy for its antioxidant, immunomodulatory, and insecticidal effects. The aerial parts of this plant include a variety of bioactive compounds. for instance, flavonoid, phenolic acids, and high levels of terpenoids. until now, wild plants are the primary source of these compounds. However, if harvesting methods are not altered soon, they may become endangered. Alternative production methods for medicinal plants using tissue culture are gaining more interest. no protocol for in vitro culture of T. daenensis has been reported so far. To address this, we carried out a study to investigate the impact of various auxins and cytokinins, both in isolation and in combination, on cell growth, development, and secondary metabolite accumulation. The objective was to assess how certain factors affect the accumulation of phenolic compounds and biomass growth in T. daenensis tissue culture. The response surface methodology modeling outcomes have shown that the use of plant growth regulators (PGRs) and their combinations significantly impacts the in vitro culture of T. daenensis. g plots summarized results showing that the best accumulation of biomass and secondary metabolites can be achieved by using 1-Naphthaleneacetic acid (NAA) followed by 2,4-Dichlorophenoxyacetic acid (2-4-D). Optimizing the induction of secondary metabolites and large-scale biomass production could greatly benefit this species.

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Section: Discussionmentioning

confidence: 99%

Modeling and optimizing plant growth regulators for in vitro culture and antioxidant activity of Thymus daenensis

samadi

2024

Preprint

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“…Arg. (HUBNER et al, 2007), Aspidosperma polyneuron (RIBAS et al, 2005) and Albizia niopoides (ROSSI;SARTORETTO, 2013).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Dalbergia nigra, an endangered Brazilian forest tree

Almeida

Silva

Cunha

2021

R. Agrogeoambiental

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We report in this work an in vitro propagation method for Dalbergia nigra using nodal segments from germinated seeds in vitro. Plant material was cultivated on MS medium supplemented with 6-benzyladeninepurine (BAP: 0 μM, 1.11 μM and 2.22 μM) and Naphthaleneacetic acid (NAA: 0.27 μM), combined with different amounts of subcultures for induction of shoot multiplication. Multiplied shoots were inoculated in MS and ½ MS medium (half strength of MS salts) to root, supplemented with NAA (0 μM and 4 μM). Data were analyzed by non-parametric statistical Kruskal-Wallis test. Best results to shoot multiplication were obtained using 1.11 μM or 2.22 μM of BAP combined 0.27 μM of NAA in multiplication medium and performing three subcultures, providing higher rates of shoots production per explant (average of 2.24) and high survival rate. Best results for rooting of shoots generated were obtained using a rooting medium containing ½ MS supplemented with 4 μM of NAA, providing a higher percentage of shoot rooting (average of 68%).

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“…A assepsia das sementes é comumente realizada com soluções de álcool (SOUZA et al, 2011;LENCINA et al, 2014) e hipoclorito de sódio (SOUZA et al, 2011;ROSSI;SARTORETTO, 2013), podendo também ser empregado o uso de soluções fungicidas (MORAES et al, 2010;ALCÂNTARA et al, 2011). Além da assepsia, as sementes de algumas espécies podem necessitar de pré-tratamentos para aumentar a porcentagem e/ou a velocidade de germinação (BRAUN et al, 2010), sendo frequentemente realizada a pré-embebição em ácido giberélico (GA 3 ) (BRAUN et al, 2010;BÁRBARA et al, 2015).…”

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Germinação in vitro de <i>Raulinoa echinata</i> R. S. Cowan (Rutaceae): sementes e embriões zigóticos

Hoffmann¹,

Bittencourt²,

Sperlich³

et al. 2022

Ciênc. Florest.

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Raulinoa echinata é uma espécie endêmica do Vale do Itajaí/Santa Catarina, ameaçada de extinção e que se encontra em ambiente vulnerável e, devido ao fato de possuir sementes recalcitrantes, as técnicas in vitro são uma das estratégias mais indicadas para sua conservação ex situ. Tendo isso em vista, o objetivo deste trabalho foi estabelecer a espécie Raulinoa echinata in vitro por meio da germinação de sementes e embriões zigóticos. As sementes foram retiradas de frutos maduros e submetidas a tratamentos de embebição em ácido giberélico (GA3) e de imersão em soluções de álcool, hipoclorito de sódio e fungicida (Benlate 500®); sendo inoculadas em meio de cultura de Murashige e Skoog (MS) sem reguladores de crescimento. Para o resgate dos embriões zigóticos, a metodologia consistiu-se em: coleta e assepsia de frutos imaturos; abertura dos frutos e retirada das sementes; assepsia das sementes; resgate dos embriões e remoção do endosperma; e inoculação dos embriões em meio de cultura MS suplementado com reguladores de crescimento: 6-benzilaminopurina (BAP), ácido 1-naftalenoacético (ANA) e GA3, isolados e em associação. As metodologias propostas para a assepsia das sementes mostram-se satisfatórias, porém não houve germinação após 65 dias de cultivo independente do tempo de embebição em GA3; formulando-se a hipótese de que o endosperma da espécie possui inibidores de crescimento que impedem a germinação in vitro. Para os embriões zigóticos, o meio de cultura MS sem reguladores de crescimento mostrou-se eficiente para a germinação (80%) e para o desenvolvimento, sendo que o futuro estabelecimento de um protocolo de criopreservação para os embriões zigóticos da espécie mostra-se promissor para a conservação ex situ.

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Propagação in vitro da farinha-seca

Cited by 6 publications

References 11 publications

Modeling and optimizing plant growth regulators for in vitro culture and antioxidant activity of Thymus daenensis

Modeling and optimizing plant growth regulators for in vitro culture and antioxidant activity of Thymus daenensis

Micropropagation of Dalbergia nigra, an endangered Brazilian forest tree

Germinação in vitro de <i>Raulinoa echinata</i> R. S. Cowan (Rutaceae): sementes e embriões zigóticos

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