Propagación in vitro de plantas adultas de Vaccinium meridionale (Ericaceae)

Cardenal, Leidy Yanira Rache; Maldonado, José Constantino Pacheco

doi:10.1590/s0102-33062010000400024

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…Castro and Álvarez (2013) in V. meridionale obtained between 66% and 80% rooting depending on the genotype. Similarly, Rache and Pacheco (2010) reported almost 90% of V. meridionale microstems with the use of IBA. In other species, such as V. corymbosum and V. vitis-idaea, in vitro rooting with the use of IBA was at 80% (Baskin et al 2000;Debnath 2003;Debnath 2005;Ostrolucká et al 2007;Ruzic et al 2012).…”

Section: In Vitro Propagationmentioning

confidence: 88%

In vitro propagation ofVaccinium floribundumKunth from seeds: promissory technology for mortiño accelerated production

2022

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The Andean mortiño (Vaccinium floribundum Kunth) grows wild in the northern paramos of South América. The berries present potential opportunities for agribusiness, but an efficient technology for the production of plants and berries is required. On the other hand, the development of plant production methods from mortiño seeds will allow the plants’ accessibility and its potential use in breeding programs. The objective of this study was to develop an efficient in vitro protocol for accelerated production using seeds. We carried out the research in four phases: seed germination, plant multiplication, rooting, and acclimatization in the greenhouse. For in vitro seed germination, we studied the effect of two culture media [woody plant medium (WPM) and Murashige and Skoog medium (MS], two photoperiods (16 and 24 hours of light), and two temperatures (18 and 28 °C). The best treatment was found to be WPM + 24 h light + 18 °C. In the micropropagation of seedlings, the effect of two concentrations of three cytokinines [(trans-zeatin riboside (TZR), zeatin (ZEA), and 2-isopentenyl adenine (2iP)], and two photoperiods (16 and 0 h light) was evaluated, whereby the concentration of 0.5 mg L−1 of TZR was the best treatment. For rooting, two doses of three auxins [indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA), and indole-3-acetic acid (IAA)] were evaluated, resulting in the 2 mg·L−1 concentration of IBA giving the best root induction. Finally, the in vitro rooted plants were acclimatized in a greenhouse. We found that peat was the best substrate. These results show that the technology developed here is useful for in vitro production of V. floribundum using seeds.

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Section: In Vitro Propagationmentioning

confidence: 88%

In vitro propagation ofVaccinium floribundumKunth from seeds: promissory technology for mortiño accelerated production

2022

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Internal morphology and histology of blueberry Vaccinium corymbosum L. (Ericaceae) in Lima, Peru

Rosa¹,

Sánchez²,

Pérez³

2017

Agron. colomb.

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Although there is a lot of information about cultivation, use and medicinal properties of blueberry Vaccinium corymbosum, there is still little information about its internal morphology and histology. Therefore, we proposed to know more of those aspects and to understand the low seed germination. The material used was composed of seeds and mature plants obtained from a farm located in Trujillo, Peru. All histological work was made in the Laboratory of Plant Anatomy and Pharmacognosy belongs to Faculty of Biological Science in Universidad Nacional Mayor de San Marcos in Lima, Peru. Each organ was analyzed in cross and longitudinal sections, as well as in external or superficial view. Lugol and Sudan IV were used for seed sections, Safranin for stem and root sections, and Lugol for leaf sections. We found some variation in seed size and color, being assigned to the category of oil seeds. Germination was limited by the embryos viability, as well as thickness of the seed coat. Stems and roots have punctuated xylem vessels, which facilitate the lateral water transport. The radical system is highly branched, apparently due to mycorrhizal symbiosis. Leaves are bifacial, with all the stomata on the abaxial side, and features that are characteristic of C3 plants.

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Micropropagación de cuatro cultivares de arándano (<i>Vaccinium</i> spp.) a partir de segmentos foliares de dos procedencias

Angulo

Matamoros

Gómez-Alpízar

2015

RAC

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Los cultivares de arándano Sharpblue, Woodard, Bonita y Delite se propagaron a partir de secciones de hoja procedentes de explantes uninodales establecidos in vitro y de secciones de hoja directamente del campo. Los explantes se colocaron en medio WPM suplementado con 1,5 mg.l-1 de TDZ para promover la brotación; luego se subcultivaron a medio fresco WPM suplementado con 0,5 ó 1,0 mg.l-1 de Zeatina, para promover el crecimiento de los brotes. Se determinó la longitud de la micromacolla y el número de brotes >2mm y >1cm por micromacolla. Brotes de 8-10 cm se cosecharon a las 8 semanas y se subcultivaron en WPM suplementado con 0, 1, 2, 4 u 8 mg.l-1 de AIB, para promover su enraizamiento; a las 10 semanas se trasplantaron a invernadero para aclimatización. Al cultivar los segmentos foliares en WPM con 1,5 mg.l-1 de TDZ se formaron micromacollas en todas las variedades, independientemente de la procedencia de la hoja, con un efecto significativo del cultivar, la procedencia, del explante y la interacción cultivar x procedencia, para el número de brotes >2 mm obtenidos. El tamaño de la micromacolla varió significativamente según cultivares, procedencia del explante e interacción cultivares x procedencia x concentración de zeatina, al trasferirse a un medio con 2 concentraciones del regulador de crecimiento, pero no hubo diferencias con respecto a éstas. El número de brotes > 2mm y la longitud del más largo variaron significativamente con el cultivar, y la interacción cultivar x procedencia del explante en WPM con 0,5 mg.l-1 de Zeatina. Explantes de Woodard a partir de vitrohoja produjeron el mayor número de brotes (44,70±13,34) y Delite de vitrohoja el menor (21,50±4,99). Los brotes subcultivados en WPM con diferentes concentraciones de AIB mostraron enraizamiento menor al 10% en todos los tratamientos. Sin embargo, al trasplantarse a invernadero se obtuvo 100% de enraizamiento, independientemente del tratamiento in vitro con AIB o la procedencia del explante. Todas las plántulas de los 4 cultivares se desarrollaron bien en invernadero y posteriormente en el campo.

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Propagación in vitro de plantas adultas de Vaccinium meridionale (Ericaceae)

Cited by 4 publications

References 24 publications

In vitro propagation ofVaccinium floribundumKunth from seeds: promissory technology for mortiño accelerated production

In vitro propagation ofVaccinium floribundumKunth from seeds: promissory technology for mortiño accelerated production

Internal morphology and histology of blueberry Vaccinium corymbosum L. (Ericaceae) in Lima, Peru

Micropropagación de cuatro cultivares de arándano (<i>Vaccinium</i> spp.) a partir de segmentos foliares de dos procedencias

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