Propagation, Expansion, and Multilineage Differentiation of Human Somatic Stem Cells from Dermal Progenitors

Bartsch, Georg; Yoo, James J.; Coppi, Paolo De; Siddiqui, Mohummad Minhaj; Schuch, Gunter; Pohl, Hans G.; Fuhr, Jasmin; Perin, Laura; Söker, Shay; Atala, Anthony

doi:10.1089/scd.2005.14.337

Cited by 142 publications

(93 citation statements)

References 34 publications

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“…Moreover, the low frequency suggests that rare tissue stem/progenitor cells that coexisted in the fibroblast cultures might have given rise to the iPSCs. Previous reports have demonstrated the presence of several types of stem cells in adult dermis with the various differentiation potential, multipotency (Bartsch et al, 2005;Shi and Cheng, 2004;Toma et al, 2001;Toma et al, 2005) or pluripotency (Chen et al, 2007). Coating of dishes with Matrigel in both CM and FFM increased the number of ALP-positive colonies (more than 10 fold vs FC and 2 fold vs non-coated dishes).…”

Section: Discussionmentioning

confidence: 90%

Feeder- and serum-free establishment and expansion of human induced pluripotent stem cells

Totonchi¹,

Taei²,

Seifinejad³

et al. 2010

Int. J. Dev. Biol.

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Although human induced pluripotent stem cells (hiPSCs) hold great promise as a source of differentiated cells for vast therapeutic implications, many obstacles still need to be surmounted before this can become a reality. One obstacle, a robust feeder-and serum-free system to generate and expand hiPSCs in culture is still unavailable. Here, for the first time, we describe a novel establishment and maintenance culture technique that uses human dermal fibroblasts to generate hiPSCs by introducing four factors, Klf4, Oct4, Sox2, and c-Myc under serum-and feeder-independent conditions. We have used a serum replacement product, conditioned medium (CM), or feeder-free medium (FFM) supplemented with high elevated basicfibroblast growth factor in the absence or presence of Matrigel. Our FFM system in the presence of Matrigel enhanced the efficiency of alkaline phosphatase-positive colonies at a frequency at least 10-fold greater than the conventional method on feeder cells. The established hiPSCs are similar to human embryonic stem cells in many aspects including morphology, passaging, surface and pluripotency markers, normal karyotype, gene expression, ultrastructure, and in vitro differentiation. Such hiPSCs could be useful particularly in the context of in vitro disease modeling, pharmaceutical screening and in cellular replacement therapies once the safety issues have been overcome.

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Section: Discussionmentioning

confidence: 90%

Feeder- and serum-free establishment and expansion of human induced pluripotent stem cells

Totonchi¹,

Taei²,

Seifinejad³

et al. 2010

Int. J. Dev. Biol.

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show abstract

“…Multipotent cell populations have been identified from different skin locations, such as epidermal stem cells from epidermis (Papini et al, 2003) and hair follicle stem cells from appendages (Rochat et al, 1994). For dermis-derived stem cells, several multipotent cell populations have been identified in rodents as well as in human beings using different culture systems (Bartsch et al, 2005;Shi and Cheng, 2004;Toma et al, 2001;Toma et al, 2005;Young et al, 1995). Toma et al have isolated multipotent precursor cells, termed skin-derived precursors (SKPs), from mammalian dermis in a suspension culture (Toma et al, 2001;Toma et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…SKPs express nestin, and can differentiate into both neural and mesodermal cell types. Recently, Bartsch et al have isolated another multipotent population, termed dermal mesenchymal stem cells (MSC), from low-temperature preserved human foreskin biopsies by adherent culture (Bartsch et al, 2005). The isolated cells could differentiate into mesodermal lineages including adipocytes, osteocytes and myocytes.…”

Section: Introductionmentioning

confidence: 99%

Clonal analysis of nestin– vimentin+ multipotent fibroblasts isolated from human dermis

Chen

Zhang

et al. 2007

Journal of Cell Science

189

168

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Although several studies have shown that dermal fibroblasts possess adipogenic, osteogenic or chondrogenic differentiation potential, no study has characterized this cell population in detail, and there is as yet no evidence that a single dermal fibroblast can differentiate into all these types of cells. In this study, dermal fibroblasts were isolated from human foreskin using a regular dermal fibroblast culture system. These cells could be expended in adherent culture for over 40 cell doublings. In addition, dermal fibroblasts exhibited adipogenic, osteogenic and chondrogenic phenotypes when they were cultured in the presence of certain inducers. Importantly, clonal analysis showed that 6.4% (3/47) of the single-cell-derived clones were tripotent, 19.1% (9/47) of the clones were bipotent, and 10.6% (5/47) of the clones were unipotent. Furthermore, one of the three tested tripotent clones exhibited neurogenic and hepatogenic differentiation potential. Phenotypic analyses showed that the tripotent fibroblasts were nestin– vimentin+, which is different from the dermis-derived stem cells reported by others. These results indicate that dermal fibroblasts are a heterogeneous population containing progenitors with various levels of differentiation potential, and the nestin– vimentin+ fibroblasts may represent a novel type of multipotent adult stem cells in human dermis.

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“…MSCs can be differentiated into multi-lineage cell types, including bone, cartilage, tendon, ligament, and muscle. 80 Moreover, the ability of MSCs to evade immunosurveillance after transplantation and to suppress the immune response has made MSCs attractive candidates for clinical use. MSCs are relatively difficult to expand in vitro, however, and they lose regenerative potential with serial passages in culture.…”

Section: Cell Sources For Use In Tissue Engineeringmentioning

confidence: 99%

Recent Applications of Polymeric Biomaterials and Stem Cells in Tissue Engineering and Regenerative Medicine

Lee

Yoo

Atala³

2014

Polymer Korea

Self Cite

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Tissue engineering and regenerative medicine strategies could offer new hope for patients with serious tissue injuries or end-stage organ failure. Scientists are now applying the principles of cell transplantation, material science, and engineering to create biological substitutes that can restore and maintain normal function in diseased or injured tissues/ organs. Specifically, creation of engineered tissue construct requires a polymeric biomaterial scaffold that serves as a cell carrier, which would provide structural support until native tissue forms in vivo. Even though the requirements for scaffolds may be different depending on the target applications, a general function of scaffolds that need to be fulfilled is biodegradability, biological and mechanical properties, and temporal structural integrity. The scaffold's internal architecture should also enhance the permeability of nutrients and neovascularization. In addition, the stem cell field is advancing, and new discoveries in tissue engineering and regenerative medicine will lead to new therapeutic strategies. Although use of stem cells is still in the research phase, some therapies arising from tissue engineering endeavors that make use of autologous adult cells have already entered the clinic. This review discusses these tissue engineering and regenerative medicine strategies for various tissues and organs.

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Propagation, Expansion, and Multilineage Differentiation of Human Somatic Stem Cells from Dermal Progenitors

Cited by 142 publications

References 34 publications

Feeder- and serum-free establishment and expansion of human induced pluripotent stem cells

Feeder- and serum-free establishment and expansion of human induced pluripotent stem cells

Clonal analysis of nestin– vimentin+ multipotent fibroblasts isolated from human dermis

Recent Applications of Polymeric Biomaterials and Stem Cells in Tissue Engineering and Regenerative Medicine

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