Propagation Methods Affect Fruit Morphology and Antioxidant Properties but Maintain Clonal Fidelity in Lowbush Blueberry

Goyali, J. C.; Igamberdiev, Abir U.; Debnath, Samir C.

doi:10.21273/hortsci.50.6.888

Cited by 26 publications

(67 citation statements)

References 55 publications

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“…At least three plants were selected randomly for each cultivar, and the leaves from each cultivar were collected in a replication of three from each plant. Five hundred milligrams of pre-frozen leaves from each plant were homogenized in a FastPrep-24 Tissue and Cell Homogenizer (MP Biomedicals, Irvine, CA, USA) in 80% aqueous acetone solution with 0.2% formic acid (1: 4 g/mL) 26 . The homogenate was kept for slow agitation at 4 °C for 30 min and then centrifuged at 13,000 rpm for 15 min at 4 °C using Allegra 64R (Beckman Coulter Inc., Palo Alto, CA, USA) before collecting the supernatant.…”

Section: Methodsmentioning

confidence: 99%

“…Total phenolic content was determined following photometric technique using the Folin-Ciocalteu method 27 , following Goyali et al . 26 . To obtain the final concentration 200 µg/mL, the crude extracts of polyphenolics were dissolved in methanol.…”

Section: Methodsmentioning

confidence: 99%

“…Total flavonoid content of blueberry samples was determined according to colorimetric method 31 following Goyali et al . 26 . 500 µL sample extract was added to the distilled water (2 mL) and 5% (w/v) sodium nitrate (150 µL).…”

Section: Methodsmentioning

confidence: 99%

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Thidiazuron-induced somatic embryogenesis and changes of antioxidant properties in tissue cultures of half-high blueberry plants

Ghosh

Igamberdiev

Debnath

2018

Sci Rep

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An efficient protocol of somatic embryogenesis (SE) has been developed for the first time in four half-high blueberry (Vaccinium corymbosum L. × V. angustifolium Ait.) cultivars. Thidiazuron (TDZ), a plant growth regulator with potential activities for shoot regeneration and shoot proliferation, was found most effective for somatic embryo formation when added to a nutrient medium at high concentration (9 µM). Although TDZ was also best for embryo germination at low concentration (2.3 µM), it was followed by zeatin at 4.6 µM for the same. Plantlets developed from SE were removed from the nutrient medium and transferred on a peat: perlite medium where 100% survival rate was acquired following the acclimatization process in a greenhouse. The concentrations of total phenolic and flavonoid contents were higher in greenhouse-grown conventionally cutting-propagated donor mother plants than those of respective SE plants for ‘St. Cloud’, ‘Patriot’ and ‘Northblue’ but not for ‘Chippewa’. The effect of propagation method and/or the older age of donor mother plants were clearly visible exclusively as the 15-year-old donor plants showed higher level of 2,2-diphenyl-1-picrylhydrazyl scavenging activity than the eight-weeks-old SE plants in all four cultivars.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Thidiazuron-induced somatic embryogenesis and changes of antioxidant properties in tissue cultures of half-high blueberry plants

Ghosh

Igamberdiev

Debnath

2018

Sci Rep

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“…Some challenges in this production are a very low rooting percentage in many genotypes, the amount of time required to propagate and commercialize newly-released cultivars for mass propagation [8,9], and phytosanitary problems. In vitro culture (micropropagation) can overcome the limitations of traditional cuttings, presenting an alternative for faster growth [10] Horticulturae 2019, 5, 24 2 of 14 throughout the year (with no seasonal effects) without pathogens [11]. There are some studies on the in vitro propagation of Vaccinium species, but only some of these have been done with rabbiteye (V. virgatum Ait.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Establishment of ‘Delite’ Rabbiteye Blueberry Microshoots

Schuchovski

Biasi

2019

Horticulturae

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Micropropagation is an important technique for clonal mass propagation and a tool for in vitro studies. One of the first steps to overcome in this process is the establishment of new explants in vitro. ‘Delite’ rabbiteye blueberry was cultured in vitro with four cytokinins (zeatin (ZEA), 6-(γ-γ-dimethylallylamino)-purine (2iP), 6-benzylaminopurine (BAP), and kinetin (KIN)) at eight concentrations (0, 2.5, 5, 10, 20, 30, 40, and 50 µM). Additionally, nine combinations of nitrogen salts were tested, using Woody Plant Medium (WPM) and a modified WPM as the basic medium. ZEA and 2iP showed better responses, but ZEA was superior at lower (2.5 µM) concentrations (89.7% survival, 81.3% shoot formation, 1.3 shoots, 13.8 mm shoot length, 10.0 leaves). BAP and KIN showed very low responses. In the combinations of salts with modified WPM, no differences were observed. However, the original WPM with treatments of 0.5 × NH4NO3 and 1 × Ca(NO3)2, 0.5 × NH4NO3 and 0.5 × Ca(NO3)2, and the modified WPM alone showed the lowest rates of survival and shoot formation and the shortest shoot lengths. The highest shoot lengths were observed in treatments with the original WPM, 1.5 × NH4NO3 and 0.5 × Ca(NO3)2, and 1.5 × NH4NO3 and 1.5 × Ca(NO3)2. This initial study with ‘Delite’ can be the basis for further experiments with different combinations of salts, 2iP, and ZEA.

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“…For example, tissue culture propagation had a significant effect on morphology and antioxidant metabolites in lowbush blueberry (Vaccinium angustifolium Ait.) compared with the use of cuttings, although the researchers did not identify any genetic changes in the 20 SSRs assessed (Goyali et al, 2015). Although not a replacement, in vitro tissue culture is one tool that may be used to supplement living collections by providing backups of plants in the ground.…”

Section: Complementary Conservation Measuresmentioning

confidence: 99%

Using Living Germplasm Collections to Characterize, Improve, and Conserve Woody Perennials

et al. 2019

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Woody perennial plants make up nearly half of plant diversity and represent one‐third of the world's major crop species, yet effective strategies to maintain and preserve these important species require additional attention. The majority of conservation programs focus on seed storage; however, seeds of many woody perennial plants are difficult to maintain in seed banks because they are recalcitrant. In addition, most woody perennial crops are clonally propagated, and seed‐based conservation efforts miss clonal lineages that form the foundation of woody perennial agriculture. Woody crops are often best maintained as living collections, but these compose only 5.8% of ex situ germplasm collections. Living germplasm collections are critical resources for exploring and conserving genetic and phenotypic diversity and provide novel material for breeding efforts. In this review, we examine how living germplasm collections can be used for for phenotypic description, genetic characterization, and plant breeding. Lastly, we outline the importance of conserving these valuable resources and highlight the need for conservation strategies that are appropriately designed for woody perennial species.

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Propagation Methods Affect Fruit Morphology and Antioxidant Properties but Maintain Clonal Fidelity in Lowbush Blueberry

Cited by 26 publications

References 55 publications

Thidiazuron-induced somatic embryogenesis and changes of antioxidant properties in tissue cultures of half-high blueberry plants

Thidiazuron-induced somatic embryogenesis and changes of antioxidant properties in tissue cultures of half-high blueberry plants

In Vitro Establishment of ‘Delite’ Rabbiteye Blueberry Microshoots

Using Living Germplasm Collections to Characterize, Improve, and Conserve Woody Perennials

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