Properties and biotechnological applications of porcine pancreatic lipase

Covalent immobilization of porcine pancreatic lipase on carboxyl-activated magnetic nanoparticles: Characterization and application for enzymatic inhibition assays

“…In particular, since its inhibitors can adjust fat absorption, lipase has become an important target in drug discovery for searching potential anti-obesity compounds [2]. Lipase obtained from pig's pancreas (porcine pancreatic lipase, PPL) is the most extensively used one due to its convenient accessibility, high stability, and broad specificity in transesterification reactions [3]. PPL is a small globular protein composed of a single chain of 449 amino acids with molecular weight of 50-52 kDa, and because it possesses 86% of homology with human pancreatic lipases, it has been usually used as a substitution for human pancreatic lipase in the enzyme inhibitor screening [4,5].…”

“…Immobilized enzymes present several benefits over the free one such as enhanced stability, ease of separation from the reaction mixture, significant savings in enzyme consumption, and usually improved activity [6]. The immobilization methods include physical adsorption, covalent attachment and entrapment in inorganic and organic matrices [3]. Up to date, PPL has been immobilized with various solid supports including macroporous anion exchange resin, magnetic microspheres, chitosan beads, porous silica beads and polyvinyl alcohol [3,7,8].…”

“…The immobilization methods include physical adsorption, covalent attachment and entrapment in inorganic and organic matrices [3]. Up to date, PPL has been immobilized with various solid supports including macroporous anion exchange resin, magnetic microspheres, chitosan beads, porous silica beads and polyvinyl alcohol [3,7,8]. The critical shortcoming of physical adsorption is that the immobilized enzymes are easily leached out from porous materials, resulting in enzyme loss and poor operational stability [9,10].…”

Produção de concentrados de ácidos graxos por hidrólise de óleos vegetais mediada por lipase vegetal

“…As lipases têm sido amplamente empregadas na produção de fármacos, emulsificantes, alimentos, perfumaria, diagnósticos médicos, compostos opticamente ativos, polímeros, aromas e fragrâncias, modificações de lipídeos para a produção de biodiesel e lipídeos estruturados e no pré-tratamento de efluentes com elevado teor de lipídeos gerados pelas indústrias de alimentos. [11][12][13][14][15] Essas enzimas encontram-se largamente distribuídas na natureza em tecidos animais e vegetais e biomassa microbiana. [11][12][13][14][15][16] Dentre elas, as lipases microbianas são as mais utilizadas industrialmente.…”

“…[11][12][13][14][15] Essas enzimas encontram-se largamente distribuídas na natureza em tecidos animais e vegetais e biomassa microbiana. [11][12][13][14][15][16] Dentre elas, as lipases microbianas são as mais utilizadas industrialmente. 6,12,13 Por outro lado, as lipases vegetais apresentam algumas vantagens em relação às lipases microbianas e animais como ampla disponibilidade, baixo custo e elevada especificidade.…”