Properties and performance of novel high-resolution/high-permeability ion-exchange media for protein chromatography

Martı́n, Cristina; Coyne, Jennifer; Carta, Giorgio

doi:10.1016/j.chroma.2004.08.114

Cited by 51 publications

(24 citation statements)

References 22 publications

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“…[ 9 ] Permeability of the monolith increased by an order of magnitude in comparison to traditional packed bed chromatography columns. [ 10 ] Therefore, polyHIPEs could be a promising solution to the problems faced by packed bed columns for biomolecule separation.…”

Section: Extending the Range Of Monomers Applicable To Emulsion Templmentioning

confidence: 99%

Functional Porous Polymers by Emulsion Templating: Recent Advances

2010

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Section: Extending the Range Of Monomers Applicable To Emulsion Templmentioning

confidence: 99%

Functional Porous Polymers by Emulsion Templating: Recent Advances

2010

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“…While the back‐pressure of monolithic capillary column decreased at higher flow rate of 2.5 mL/min because a small collapse was found in the continuous bed. The permeability of two monoliths was calculated based on slopes of back‐pressure versus flow rate (Darcy's law) , the results are shown in Table . The monolith frit was less permeable than the monolith capillary, this less permeablility of monolith frit may be resulted from the partially ‘‘clogged’’ by totally impermeable polypropylene in frit.…”

Section: Resultsmentioning

confidence: 99%

Development of a novel monolith frit‐based solid‐phase microextraction method for determination of hexanal and heptanal in human serum samples

Yan²,

Song³

2012

J of Separation Science

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In this paper, a polypropylene frit with porous network structure and high area-to-thickness ratio (4.8 mm diameter, 1.6 mm thickness, 20 mm pore size) was utilized as a mould of monolith. Poly(methacrylic acid-ethlyene glycol dimethacrylate) (MAA-EGDMA) monolith was in situ synthesized in the micro-channel of frit by photopolymerization. A monolith frit-based solid-phase microextraction method (SPME) was developed for the determination of hexanal and heptanal in serum samples by combining with high-performance liquid chromatography. 2,4-Dinitrophenylhydrazine (DNPH) as the derivatizing reagent was absorbed on a monolith frit, then its derivatization reaction with aldehydes and the absorption of formed hydrazones on the monolith disk occurred simultaneously. The condition parameters for polymerization, derivatization and extraction were optimized systematically. Under the optimum conditions, rigid structure, low back-pressure and high column capacity were achieved for the monolith frit. The limits of detection for hexanal and heptanal were 1.86 and 1.38 nmol/L, respectively. The inter- and intra-day relative standard deviations were less than 7.7% (n = 6). This method was applied successfully to aldehydes analysis in human serum samples. The method possesses advantages such as simplicity, efficiency, low cost and good biocompatibility. It provides an alternative approach for quantification of aldehydes in complex biological samples.

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“…Back pressure under different flow rates of the prepared heparin‐immobilized polyHIPE monolith was determined on a Series III Digital Pump (USA). Permeability of the prepared heparin‐immobilized polyHIPE monolith was calculated based on Darcy's law: K = µηL/Δp, where Δp stood for back pressure (Pa), L for column length (m), η for superficial velocity (m s −1 ), µ for mobile phase viscosity (Pa·s), and K for permeability (m 2 ). The value of K could be used as an index for evaluating the quality of monolith.…”

Section: Methodsmentioning

confidence: 99%

Heparin‐Immobilized Polymeric Monolithic Column with Submicron Skeletons and Well‐Defined Macropores for Highly Efficient Purification of Enterovirus 71

Liu

Yin

et al. 2018

Macro Materials & Eng

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In this study, glycidyl methacrylate‐based polymerized high internal phase emulsion monolithic column (polyHIPE) with submicron skeletons and well‐defined macropores is prepared and functionalized by immobilizing heparin to obtain a novel affinity chromatographic separation medium for effective purification of Enterovirus 71 (EV71). The pore structure of the obtained monoliths differs significantly from conventional polyHIPE monoliths. The prepared polyHIPE presents well‐defined 3D network skeletons of 0.2–0.5 µm, interconnected macropores of 0.5–2 µm, large specific surface area (47–61 m2 g−1), and excellent permeability (k = 1.0 × 10−13 m2). Further, successful immobilization of heparin is proved by FTIR and XPS and the monoliths display superiority in the purification of EV71 from culture supernatant. The final recovery and binding capacity of EV71 are calculated to be 60% and 152.8 ng per column, respectively. SDS‐PAGE gel electrophoresis and TEM analysis indicate a high efficiency in the removal of impurities from culture supernatant of EV71. This, together with easy fabrication, makes polyHIPE monolith a promising alternative to commercially available monolithic supports for virus purification.

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Properties and performance of novel high-resolution/high-permeability ion-exchange media for protein chromatography

Cited by 51 publications

References 22 publications

Functional Porous Polymers by Emulsion Templating: Recent Advances

Functional Porous Polymers by Emulsion Templating: Recent Advances

Development of a novel monolith frit‐based solid‐phase microextraction method for determination of hexanal and heptanal in human serum samples

Heparin‐Immobilized Polymeric Monolithic Column with Submicron Skeletons and Well‐Defined Macropores for Highly Efficient Purification of Enterovirus 71

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