Properties of &amp;lt;i&amp;gt;Bacillus&amp;lt;/i&amp;gt; species Cellulase Produced Using Cellulose from Brewers Spent Grain (BSG) as Substrate

Orji, F. A.; Dike, E. N.; Lawal, A. K.; Sadiq, Abdulateef Omoniyi; Suberu, Yewande; Famotemi, Ariyo Caleb; Ugbana, Agatha Isioma; Fashola, Folake; Ita, Blossom; Olatope, S.O.A.; Itoandoan, Emoleila Ejiya; Adefiranye, Abimbola Olajumoke; Elemo, Gloria N.

doi:10.4236/abb.2016.73013

Cited by 8 publications

(8 citation statements)

References 6 publications

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“…Cellulases generated by various microorganisms usually differ in the optimum conditions for their highest activity. Simultaneously, these conditions may considerably differ from the optimum conditions of growth and development [27,32,34]. The enzyme active centre is a widened loop, which creates a tunnel for one of the hydrolised ends of cellulose chain.…”

Section: Cellulolytic Enzymesmentioning

confidence: 99%

“…Another method of cellulolytic activity assessment is determining of FPase, so called total cellulolytic activity. Similarly to assessment of CMCase activity, volumes of individual components of the reaction mixture (cm 3 of enzyme, buffer, DNS, H 2 O) and reaction time may slightly differ [10,16,21,26,34,49].…”

Section: Total Cellulolytic Activity Of Microorganisms -Determining Fpase Activitymentioning

confidence: 99%

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Determining Cellulolytic Activity of Microorganisms

Grata

2020

Chemistry-Didactics-Ecology-Metrology

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Decomposition of cellulose to glucose requires complex cooperation of glycoside hydrolase enzymes. As a result of glycoside β-1,4 bonds hydrolysis, shorter chains of cellulose, oligodextrin, cellobiose and glucose are created. A number of bacteria and fungi demonstrate the capacity to degrade cellulose. Their activity can be assessed with the use of qualitative and quantitative methods. Qualitative methods with the use of e.g. Congo red, are used in screening studies, however, they do not provide information about the quantity of the produced enzyme. Spectrophotometric methods are more accurate and they measure the quantities of reducing sugars with the use of appropriate substrates, e.g. carboxymethylcellulose is used to determine endoglucanases, avicel cellulose to determine exoglucanases and Whatman filter paper to determine total cellulolytic activity. Activity of microorganisms depends not only on their species or type but also, among others, on substratum composition, cultivation conditions and the appropriate selection of parameters of the carried out enzymatic reactions.

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Section: Cellulolytic Enzymesmentioning

confidence: 99%

Section: Total Cellulolytic Activity Of Microorganisms -Determining Fpase Activitymentioning

confidence: 99%

Determining Cellulolytic Activity of Microorganisms

Grata

2020

Chemistry-Didactics-Ecology-Metrology

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“…Hal ini tidak mengejutkan, karena karbon merupakan sumber energi bagi kebanyakan bakteri di lingkungan, termasuk pada saat menjalankan peran pada siklus nitrogen di alam (Wilkinson, 1963). Beberapa jenis bentuk karbon di dalam sedimen mampu digunakan oleh bakteri selama proses respirasi, dan dilepaskan dalam bentuk CO2 (2001;Orji et al, 2016). Begitupula keberadaan nitrogen juga memiliki kaitan erat dengan keberadaan bakteri di lingkungan, dimana nitrogen dalam berbagai bentuk sangat dibutuhkan oleh bakteri sebagai nutrien.…”

Section: Pembahasanunclassified

Dekomposisi bahan organik pada sedimen di Tukad Mati dan Tukad Badung, Bali

Perwira¹,

Ekawaty²,

Sari³

et al. 2019

J. Mar. Aquat. Sci.

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The sediment properties of Mati and Badung River, Bali were observed in this study. This study was aimed to know the relation between carbon, nitrogen and total bacterial number in the sediment of those rivers. This study was carried out in Mati and Badung River located in Southern part of Bali, Indonesia. Total carbon (TC), total nitrogen (TN), and total bacterial number of the sediment were observed in this study. Slow stirring method was used to estimate the total bacterial number in sediment, based on their environmental DNA intensity after agarose gel electrophoresis. TC of sediment was analyzed by using total carbon analyzer after combustion at 900oC, while TN was analyzed by using indophenol blue method after digested using Kjeldahl at 420oC for 1.5 hours. The result showed that TC of sediment in the Mati and Badung River were 6,100 and 7,000 mg/kg, respectively. TN of sediment in the Mati and Badung River were 380 and 440 mg/kg, respectively. The pH and electrical conductivity (EC) of sediment in the Mati River were 6,88 dan 0,10 µs/cm, while in the Badung River were 6,80 dan 0,11 µs/cm. Total bacterial number in the Mati river was lower (4.8 × 108 cell /g) than that in the Badung river (3.8 × 108 cell/g). The high value of R2 between total bacterial and TC (0.91), and between total bacterial number and TN (0.83), indicating the high influence of bacteria on the decomposition of organic materials in the sediment.

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“…Enzim selulase ekstraseluler dari Bacillus sp. B233 juga memiliki kondisi optimum pada pH 5 (Orji et al, 2016). (Meryandini dkk, 2009).…”

Section: Uji Aktivitas Enzimunclassified

Isolasi Dan Karakterisasi Parsial Enzim Selulase Bakteri Selulolitik Hasil Isolasi Dari Limbah Padat Tapioka (Onggok)

Putri¹,

Wardani²

2018

ATJ

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Proses hidrolisis bahan berselulosa tinggi memerlukan perlakuan pendahuluan untuk memisahkan lignin dan hemiselulosa dari selulosa, tetapi proses ini kurang efisien dan tidak ramah lingkungan. Enzim selulase adalah alternatif untuk menghidrolisa selulosa karena sifat kerja enzim yang spesifik dan tidak menghasilkan limbah yang berbahaya serta diharapkan mampu membantu memudahkan proses perlakuan pendahuluan. Bakteri selulolitik penghasil enzim selulase dapat diisolasi dari limbah pertanian yang banyak mengandung selulosa seperti limbah padat tapioka (onggok). Hasil penelitian menunjukkan bahwa 2 isolat yaitu TO4 dan TO5 merupakan isolat yang potensial menghasilkan selulase. Enzim selulase dari isolat TO4 dan TO5 memiliki aktivitas optimum pada pH 5 dan suhu 50°C dengan aktivitas enzim selulase sebesar 0,597 U/mg untuk TO4 dan 0,557 U/mg untuk TO5. Hasil uji kemampuan enzim dalam hidrolisis bahan berselulosa tinggi seperti bagas tebu menunjukkan bahwa enzim selulase dari isolat TO4 mampu menghidrolisis selulosa pada bagas tebu sebanyak 15,8% setelah direaksikan selama 120 jam.

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Properties of <i>Bacillus</i> species Cellulase Produced Using Cellulose from Brewers Spent Grain (BSG) as Substrate

Cited by 8 publications

References 6 publications

Determining Cellulolytic Activity of Microorganisms

Determining Cellulolytic Activity of Microorganisms

Dekomposisi bahan organik pada sedimen di Tukad Mati dan Tukad Badung, Bali

Isolasi Dan Karakterisasi Parsial Enzim Selulase Bakteri Selulolitik Hasil Isolasi Dari Limbah Padat Tapioka (Onggok)

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