Properties of Bac W42, a Bacteriocin Produced by Bacillus subtilis W42 Isolated from Cheonggukjang

Kindoli, Salum; Lee, Hwang A; Kim, Jeong Hwan

doi:10.4014/jmb.1110.10002

Cited by 24 publications

(14 citation statements)

References 35 publications

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“…Many suitable fermentative Bacillus species have recently been recovered from CKJ or CKJ-related products, and functional activities of soy foods fermented with Bacillus species may be associated with total contents and/or types of various components such as isoflavones [14,22,23]. To identify suitable fermentative bacteria, we prepared CKJ using B. subtilis MC31 and L. sakei 383.…”

Section: Discussionmentioning

confidence: 99%

Toxicity of fermented soybean product (cheonggukjang) manufactured by mixed culture ofBacillus subtilisMC31 andLactobacillus sakei383 on liver and kidney of ICR mice

et al. 2014

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To investigate the toxic effects of cheonggukjang (CKJ) manufactured using mixed cultures of Bacillus subtilis MC31 and Lactobacillus sakei 383 on the liver and kidney of ICR mice, an alteration on the related markers including body weight, organ weight, urine composition, liver pathology and kidney pathology were analyzed after oral administration at dosage of 25, 50 and 100 mg/kg body weight/day of CKJ for 14 days. Any significant toxicity was not observed on the body and organ weight, clinical phenotypes, urine parameters and mortality in the CKJ-treated group compared with the vehicle-treated group. Also, liver toxicity analysis revealed no significant increase in alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST) or lactate dehydrogenase (LDH) in response to CKJ. Additionally, the specific pathological features induced by most toxic compounds were not observed upon liver histological analysis. Furthermore, kidney toxicological analysis revealed that blood urea nitrogen (BUN) and the serum creatinine (Cr) levels and pathological features on histological sections did not differ significantly between the vehicle- and CKJ-treated groups. Overall, these results suggest that CKJ does not induce any specific toxicity in liver and kidney organs of ICR at dose of 100 mg/kg body weight/day as no observed adverse effect level (NOAEL).

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Section: Discussionmentioning

confidence: 99%

Toxicity of fermented soybean product (cheonggukjang) manufactured by mixed culture ofBacillus subtilisMC31 andLactobacillus sakei383 on liver and kidney of ICR mice

et al. 2014

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“…Recently, B. subtilis was used in many applications, such as biosorption of heavy metals (Abdel-Monem and Al-ZubeiryAH 2010), fermentation of red bean by a fibrinolytic enzyme (Chang et al 2012), and production of bacteriocin (Kindoli et al 2012), phenolic acid decarboxylase (Frank et al 2012) and chitinase enzyme (Chandrasekaran et al 2012). Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of Pharmaceutical Wastes in Treated Sewage Effluents by Bacillus subtilis 1556WTNC

Al-Gheethi¹,

Ismail²

2014

Environ. Process.

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The aim of this study was to investigate the potentials for enzymatic biodegradation of pharmaceutical active compounds (β-lactam antibiotics) in treated sewage effluents as a function of β-lactamase produced Bacillus subtilis 1556WTNC. Four β-lactams antibiotics were selected: two of them belong to penicillin's (amoxicillin and ampicillin) and two belong to cephalosporins (cephalexin and cefuroxime); ciprofloxacin (belongs to quinolones) was used as a negative control. The enzymatic biodegradation process was conducted under the optimal conditions for β-lactams production (5.9 log 10 CFU mL −1 ; pH 6.5; temperature 35°C for 12 days) as determined in this research. The maximum biodegradation was 25.03 % at 1 mg mL −1 for amoxicillin, 15.59 % at 0.8 mg mL −1 of ampicillin, 22.59 % at 1 mg mL −1 of cephalexin, 10.62 % at 1 mg mL −1 of cefuroxime, while it was 2.45 % at 0.6 mg mL −1 of ciprofloxacin. B. subtilis 1556WTNC exhibited the potential to produce β-lactamase and biodegrade β-lactam antibiotic genetically and inducibly B. subtilis 1556WTNC could grow and biodegrade β-lactam antibiotics in conditions similar to the characteristics of treated sewage effluents such as pH, temperature, and during short time (12 days), because it was already acclimatized to those conditions. For this reason, treated sewage effluents were used as source to isolate this strain. It can be concluded that B. subtilis 1556WTNC is suitable to remove pharmaceutical residues from the treated sewage effluents and produce effluents at higher quality than that achieved by secondary treatment process.

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“…The absorbance was measured at 550 nm every hour up to 12 h. The viable cell count was also measured 6 and 10 h after bacteriocin addition. L. monocytogenes without bacteriocin served as control (Gao et al, 2011;Kindoli et al, 2012).…”

Section: Mode Of Actionmentioning

confidence: 99%

Bacteriocin activity against various pathogens produced by Pediococcus pentosaceus VJ13 isolated from Idly batter

Vidhyasagar

Jeevaratnam

2013

Biomedical Chromatography

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Bacteriocins, an antimicrobial peptide, is known to have wide spectrum antimicrobial activity against various pathogens. Because they are easily digested in the intestine, they are considered as safe and are widely used as food preservatives. Hence their purification and characterization have attracted considerable attraction, especially for those having activity against human pathogens. In this study, the bacteriocin produced by Pediococcus pentosaceus VJ13 was precipitated with cold acetone and purified by gel permeation chromatography and hydrophobic interaction chromatography. The bacteriocin exhibited antimicrobial activity against various pathogens, like Mycobacterium smegmatis, Klebsiella pneumonia, Clostridium perfringens and Staphylococcus epidermidis. The activity of bacteriocin was lost completely after treatment with protease, which revealed its proteinaceous nature. The bacteriocin was stable up to 100°C and exhibited antilisterial property which is a characteristic feature of class IIa bacteriocins. It was active within the pH range of 2-8 and stable against various chemicals and denaturants. Tricine SDS-PAGE revealed its molecular weight to be 4.0 kDa, where the corresponding activity against Listeria monocytogenes was also noted. Treatment of L. monocytogenes with bacteriocin decreased the viable cell count, and scanning electron microscope analysis revealed membrane pore formation that resulted in the release of intracellular content, suggesting its bactericidal effect.

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Properties of Bac W42, a Bacteriocin Produced by Bacillus subtilis W42 Isolated from Cheonggukjang

Cited by 24 publications

References 35 publications

Toxicity of fermented soybean product (cheonggukjang) manufactured by mixed culture ofBacillus subtilisMC31 andLactobacillus sakei383 on liver and kidney of ICR mice

Toxicity of fermented soybean product (cheonggukjang) manufactured by mixed culture ofBacillus subtilisMC31 andLactobacillus sakei383 on liver and kidney of ICR mice

Biodegradation of Pharmaceutical Wastes in Treated Sewage Effluents by Bacillus subtilis 1556WTNC

Bacteriocin activity against various pathogens produced by Pediococcus pentosaceus VJ13 isolated from Idly batter

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