Properties of Branching Enzyme from Hyperthermophilic Bacterium, Aquifex aeolicus, and Its Potential for Production of Highly-branched Cyclic Dextrin.

Takata, Hitoshi; Ohdan, Kohji; Takaha, Takeshi; Kuriki, Takashi; Okada, Shigetaka

doi:10.5458/jag.50.15

Cited by 66 publications

(49 citation statements)

References 16 publications

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“…Both proteins were stable up to 90°C, and activities decreased gradually during incubation at 100°C. The thermostability was even higher than that of A. aeolicus BE, which is the most thermostable BE previously reported, with stability at temperatures up to 85°C (38). In Fig.…”

Section: And 5)mentioning

confidence: 86%

“…After incubation, HCl was added to a final concentration of 16 mM, and the mixture was further incubated for 10 min at 100°C. Glucans in the reaction mixture were analyzed by high-performance liquid chromatography (HPLC) using a multiangle laser-light-scattering photometer (DAWN DSP; Wyatt Technology, Santa Barbara, CA) and a differential refractive-index detector (RI-71; Showa Denko, Tokyo, Japan), as described by Takata et al (38).…”

Section: Bioinformaticsmentioning

confidence: 99%

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A Novel Branching Enzyme of the GH-57 Family in the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1

et al. 2006

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Branching enzyme (BE) catalyzes formation of the branch points in glycogen and amylopectin by cleavage of the ␣-1,4 linkage and its subsequent transfer to the ␣-1,6 position. We have identified a novel BE encoded by an uncharacterized open reading frame (TK1436) of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. TK1436 encodes a conserved protein showing similarity to members of glycoside hydrolase family 57 (GH-57 family). At the C terminus of the TK1436 protein, two copies of a helix-hairpin-helix (HhH) motif were found. TK1436 orthologs are distributed in archaea of the order Thermococcales, cyanobacteria, some actinobacteria, and a few other bacterial species. When recombinant TK1436 protein was incubated with amylose used as the substrate, a product peak was detected by high-performance anion-exchange chromatography, eluting more slowly than the substrate. Isoamylase treatment of the reaction mixture significantly increased the level of short-chain ␣-glucans, indicating that the reaction product contained many ␣-1,6 branching points. The TK1436 protein showed an optimal pH of 7.0, an optimal temperature of 70°C, and thermostability up to 90°C, as determined by the iodine-staining assay. These properties were the same when a protein devoid of HhH motifs (the TK1436⌬H protein) was used. The average molecular weight of branched glucan after reaction with the TK1436⌬H protein was over 100 times larger than that of the starting substrate. These results clearly indicate that TK1436 encodes a structurally novel BE belonging to the GH-57 family.

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Section: And 5)mentioning

confidence: 86%

Section: Bioinformaticsmentioning

confidence: 99%

A Novel Branching Enzyme of the GH-57 Family in the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1

et al. 2006

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show abstract

“…Both proteins showed the same optimal pH value of 7.0, functioned optimally at a temperature of 70 C, and had the same specific activities (TK1436∆ H protein had a 95.6±4.6% activity of TK1436 protein). Both proteins were stable up to 90 C and activities decreased gradually during incubation at 100 C. The thermostability is even higher than that of A. aeolicus BE, which is the most thermostable BE previously reported, with stability at temperatures up to 85 C. 29) In Fig. 3, the data on TK1436 ∆ H protein are shown as representative.…”

Section: Enzymatic Characterization Of Recombinant Tk1436 and Tk1436 mentioning

confidence: 90%

A Novel Branching Enzyme of the GH-57 Family

保¹,

平²,

洋樹³

et al. 2007

J. Appl. Glycosci.

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“…BE from Aquifex aeolicus was partially purified, as described previously. 14) The mixture was heated at 90 C for 30 min after adjusting pH to 4.5 with HCl to stop the reaction, and then centrifuged. The supernatant was subjected to ultrafiltration (500 kDa molecular mass cut-off membrane module, type FS03-FC-FUS5082, Daisen Membrane Systems Ltd., Tokyo, Japan) to remove fructose and ions from glycogen.…”

Section: Methodsmentioning

confidence: 99%

Anti-Tumor Activity of an Enzymatically Synthesized α-1,6 Branched α-1,4-Glucan, Glycogen

Ryoyama

Kidachi

Yamaguchi

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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Properties of Branching Enzyme from Hyperthermophilic Bacterium, Aquifex aeolicus, and Its Potential for Production of Highly-branched Cyclic Dextrin.

Cited by 66 publications

References 16 publications

A Novel Branching Enzyme of the GH-57 Family in the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1

A Novel Branching Enzyme of the GH-57 Family in the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1

A Novel Branching Enzyme of the GH-57 Family

Anti-Tumor Activity of an Enzymatically Synthesized α-1,6 Branched α-1,4-Glucan, Glycogen

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