Properties of cells derived from adenovirus-induced hamster tumors by long-term in vitro cultivation

Rouse, Harriet; Strohl, William A.; Schlesinger, R. Walter

doi:10.1016/0042-6822(66)90248-0

Cited by 40 publications

(5 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is evidence that this defect is due, at least in part, to the failure of the major late promoter of Adl2 DNA to function in hamster cells, whereas the same promoter is operative in human cells and the major late promoter of Ad2 DNA is capable of functioning in both hamster and human cells (31). Similarly, Ad2 is capable of replicating in both cell types (7,21).…”

mentioning

confidence: 99%

Defect of adenovirus type 12 replication in hamster cells: absence of transcription of viral virus-associated and L1 RNAs

Jüttermann

Weyer

Doerfler

1989

J Virol

View full text Add to dashboard Cite

The nonpermissive interaction of hamster cells with human adenovirus type 12 (Adl2) is characterized by a total block of Adl2 DNA replication and late transcription, whereas most of the early functions of Adl2 DNA can be transcribed. Ad2 can replicate in hamster cells. The replication and late transcription defects of Adl2 DNA can be complemented to a certain extent by the E1B functions of Ad2 DNA. This complementation fails, however, to lead to the synthesis of the late Adl2 proteins and to the assembly of infectious virions. It will now be demonstrated that the Adl2 Li (late genes of group 1) and virus-associated (VA) RNAs are not transcribed in hamster cells. Synthesis of these RNAs in productively infected human cells or Ad2-infected hamster cells is readily detectable by Si nuclease protection experiments and Northern (RNA) blotting. Similarly, the Ad2-transformed hamster cell line BHK-Ad2E1 fails to complement LI and VA RNA syntheses after superinfection with Adl2. However, Adl2 infection of the Ad5-transformed hamster cell line BHK297-C131 leads to the transcription of the Adi2 Li and VA segments. This difference in complementation by the two transformed hamster cell lines might be accounted for by functions in the segment of Ad5 DNA extending between map units 30 and 40 and persisting in the Ad5-transformed hamster cells or by hamster host cell functions which might be operative in cell line BHK297-C131 but not in BHK-Ad2E1 or BHK-21 hamster cells.

show abstract

mentioning

confidence: 99%

Defect of adenovirus type 12 replication in hamster cells: absence of transcription of viral virus-associated and L1 RNAs

Jüttermann

Weyer

Doerfler

1989

J Virol

View full text Add to dashboard Cite

show abstract

“…In contrast to Adl2, human adenovirus type 2 (Ad2) can replicate and grow to substantial titers in hamster cells (10,32,42). We therefore asked whether Ad2 or Adl2 DNA can replicate in Ad2 and Adl2 double-infected BHK-21 cells.…”

mentioning

confidence: 99%

Adenovirus types 2 and 5 functions elicit replication and late expression of adenovirus type 12 DNA in hamster cells

Klimkait¹,

Doerfler²

1985

J Virol

View full text Add to dashboard Cite

Cells and virus. The following cell lines were propagated in Dulbecco modified Eagle medium (3) supplemented with 7 to 10% fetal bovine serum: BHK-21 cells, subline B3 (41), and the Ad2-transformed hamster cell lines HE1, HE3, HE5, and HE7 (7), as well as human HeLa cells (ATCC, CCL 2). Human KB cells (ATCC, CCL 17) were grown in Eagle medium containing 10% newborn calf serum. The AdStransformed cell line BHK297-131 (47), a gift of J. Sussenbach, Utrecht, The Netherlands, was grown in the Dulbecco modified Eagle medium containing 7% fetal calf serum. Cell lines HEl, HE3, HES, and HE7 were gifts of A

show abstract

“…Similar neoantigens may be present in cells transformed by adenovirus 12 or adenovirus 18 (Gilead & Ginsberg, 1965), and Rouse, Strohl & Schlesinger (1966) have shown that thirteen clones of two cell lines derived from hamster tumour cells transformed by type 12 adenovirus all had transplantation protein antigen. Cells transformed by adenovirus often continue to elaborate adenovirus and viral antigen (Pope & Rowe, 1964), so that the distinction between viral antigen and neoantigen is not so easily made as with polyoma, in which transformed cells do not contain free virus.…”

Section: Hep-2 Cells Which Was Not Present On Uncultured Human Amnionmentioning

confidence: 92%

Antigens as Markers on Cultured Mammalian Cells

Franks

1968

Biological Reviews

View full text Add to dashboard Cite

Properties of cells derived from adenovirus-induced hamster tumors by long-term in vitro cultivation

Cited by 40 publications

References 14 publications

Defect of adenovirus type 12 replication in hamster cells: absence of transcription of viral virus-associated and L1 RNAs

Defect of adenovirus type 12 replication in hamster cells: absence of transcription of viral virus-associated and L1 RNAs

Adenovirus types 2 and 5 functions elicit replication and late expression of adenovirus type 12 DNA in hamster cells

Antigens as Markers on Cultured Mammalian Cells

Contact Info

Product

Resources

About