Properties of endogenous somatostatin-like immunoreactivity and synthetic somatostatin in dog plasma.

A B S T R A Clevel at 15-20 wk of gestation (320±55 pg/ml, n = 15) being 4.5 times higher than the mean at 32-43 wk (70±12 pg/ml, n = 15) (P < 0.001). In cases of preeclampsia (n = 6), gestational diabetes (n = 5), anencephaly (n = 1), and meningomyelocele (n = 1), SLI values were in the normal range, but in one juvenile diabetic and one patient with chronic renal failure, SLI was undetectable (<10 pg/ml). In a pair of monochorionic diamniotic twins, SLI levels were very different (33 and 197 Received for publication 29 January 1979 and in revised form 18 May 1979. of total amniotic fluid SLI) were found in cells from fresh fluid. Radioimmunoassay of SLI in extracts of seven paired cord arterial and venous plasma samples showed no arteriovenous gradient consistent with fetal origin ofcord blood SLI. It is concluded that (a) amniotic fluid contains SLI which is offetal origin and (b) normal levels vary with gestational age. The SLI has a higher molecular weight (-5,000) and is more stable in amniotic fluid than SRIF.

Section: Discussionmentioning

confidence: 95%

Measurement, Characterization, and Source of Somatostatin-like Immunoreactivity in Human Amniotic Fluid

Fitzpatrick¹,

Patel²

1979

“…1). During the course of the initial viability studies as well as in these and the subsequent IRS perfusions, no visible hemolysis was observed in perfusate supernatant fluids, with the exception noted at perfusion pH 6 Effect of lowered pH on IRS clearance at 37°C. Table II shows that when the mean perfusate pH was adjusted from 7.40 to 7.06, t112 lengthened from 20.0 ±1.3 to 28.7±3.2 min, hepatic clearance fell from 0.84±0.04 to 0.54±0.08 ml/min per g, and extraction was reduced from 36±2% to 26±3%.…”

Section: Calculatiotismentioning

confidence: 92%

“…Concentrations of immunoreactive somatostatin (IRS)' in serum or plasma from man (1) and animals (2)(3)(4)(5)(6)(7) are higher in the hepatic portal vein or its major tributaries than in simultaneous samples drawn from the hepatic or peripheral systemic veins. Although the absolute concentrations ofIRS are lower ifthe sample is extracted before the assay (-20-600 pg/ml) (1,2,4) than not (-300-2,000 pg/ml) (3,5), the values suggest that hepatic extraction of IRS might accouint for the negative transhepatic gradients that have also been shown previously for insulin (8) and glucagon (9,10).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Clearance of Immunoreactive Somatostatin by Perfused Rat Liver

Sacks¹,

Terry²

1981

“…Somatostatin was measured by a modification (10) of the methods of Kronheim et al (11) and Arimura et al (12). This procedure has been validated for use in unextracted dog plasma (13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Plasma somatostatin-like immunoreactivity during the interdigestive period in the dog.

Aizawa¹,

Harris²,

Unger³

1981

Self Cite

A B S T R A C T To study possible physiologic relationships between somatostatin and the gastric interdigestive contractions (GIC), gastric motor activity, and plasma somatostatin-like immunoreactivity (SLI) concentration were determined simultaneously in four conscious dogs, each of which was studied on two separate occasions. Plasma SLI level was highest during the GIC period and lowest 60 and 80 min after the cessation of the GIC; the mean difference in plasma SLI was 41+6 pglml. When synthetic motilin, a known stimulus of GIC, was infused at a physiologic rate during the period in which plasma SLI levels were low, SLI rose to approximately the same values observed during the contraction period and GIC similar to those that occur spontaneously were observed. When synthetic somatostatin, a known inhibitor of endogenous motilin release, was infused at a rate that raised the plasma SLI to approximately the levels observed during the contraction period (0.1 gg/kg per h), the appearance of the subsequent GIC was significantly delayed.These results are consistent with a physiological role for somatostatin in the regulation of GIC in dogs and suggest an interrelationship between motilin and somatostatin.