Properties of Ethanolysis Product from Ketapang Seed Oil (Terminalia Catappa Linn) Incorporated in Mucoadhesive Patch Film

Abstract

The propose of these research was to prepare monoacilglyserol (Ethanolysis Product from Ketapang Seed Oil/EPKSO) by ethanolysis reaction and to measured it’s antibacterial activity as free materials and as immobilizes material in starch film/patch. The oil of ketapang seed was extracted by pressing method. Fatty acid in the oil was identified with Gas Chromatography (GC). Ethanolysis process used 5 mL ketapang seed oil and 2mL NaOH in 100 mL ethanol 95%. Sample EPKSO from ethanolysis of ketapang seed oil were compare to EPKSO standard from SEAFAST Centre. The preparative TLC of ethanolysis product was eluted with n-hexane: diethyleter: formic acid (80:20:2) v/v. EPKSO was produced by TLC preparative from ethanolysis product. Antibacterial activity of EPKSO from ethanolysis reaction was assayed against Streptococcus mutans with diffusion method. From this research, ketapang seed oil obtained was 7.69 % w/w. The major fatty acid from ketapang seed oil from Gas Chromatography (GC) were palmitic acid, stearic acid, oleic acid and linoleic acid. The minor of fatty acid were miristic acid, palmitoleic acid, heptadecanoic acid, cis-10-heptadecanoic acid, belaidic acid, arachidonic acid, cis-11-eicosenoic acid, linoleic acid, cis-11.14-eicosedienoic acid, behenic acid, trisanoic acid and lignoseric acid. Both of the EPKSO have similar Rf (0.07). The amount of EPKSO result from ethanolysis ketapang seed oil was 1.79 % w/w. This EPKSO can inhibit S. mutans at 0.4 % w/v with inhibition zone 183 mm2. EPKSO was incorporated in starch based film/mucoadhesive patch with variation 2 MIC, 4 MIC 6 MIC dan 8 MIC. After incorporated with EPKSO starch based film/mucoadhesive patch can inhibit the growth of Streptococcus mutans at all EPKSO concentration in film/patch. Most of mechanical properties of film/patch still meet the range of standard. Organoleptic assay showed no significant difference between starch film with and without EPKSO.