Properties of peptides released from salmon and carp via simulated human-like gastrointestinal digestion described applying quantitative parameters

Abstract

Apart from the classical (experimental) methods, biologically active peptides can be studied via bioinformatics approach, also known as in silico analysis. This study aimed to verify the following research hypothesis: ACE inhibitors and antioxidant peptides can be released from salmon and carp proteins during simulated in silico human-like gastrointestinal digestion. The potential to release biopeptides was evaluated using the BIOPEP-UWM quantitative criteria including the profile of biological activity, frequency of the occurrence (A)/release (AE) of fragments with an ACE inhibitory or antioxidant activity by selected enzymes, and relative frequency of release of bioactive fragments with a given activity by selected enzymes (W). Salmon collagen and myofibrillar proteins of carp turned out to be the best potential source of the searched peptides–ACE inhibitors and antioxidant peptides. Nonetheless, after digestion, the highest numbers of ACE inhibitors and antioxidant peptides were potentially released from the myofibrillar proteins of salmon and carp. Peptide Ranker Score, Pepsite2, and ADMETlab platform were applied to evaluate peptides’ bioactivity potential, their safety and drug-like properties. Among the 63 sequences obtained after the simulated digestion of salmon and carp proteins, 30 were considered potential biopeptides. The amino acid sequences of ACE-inhibiting and antioxidant peptides were predominated by P, G, F, W, R, and L. The predicted high probability of absorption of most analyzed peptides and their low toxicity should be considered as their advantage.

An updated review on food‐derived bioactive peptides: Focus on the regulatory requirements, safety, and bioavailability

“…It focuses on the kinetics of identifying if a molecule will reach its target protein in the body and how long it will remain in a bloodstream. Consequently, this technology may be used to analyze peptides, which can then be employed as components of functional foods or supplements (Borawska‐Dziadkiewicz et al., 2021). A number of in silico tools are available to assess ADMET properties of peptides comprising admetSAR (http://lmmd.ecust.edu.cn/admetsar1/), ADMETlab platform (http://admet.scbdd.com/), iDrug (https://drug.ai.tencent.com/cn), SwissADME (http://www.swissadme.ch), and so forth.…”

“…The ADMET prediction findings indicated that these predicted peptides could be utilized as orally ACE‐inhibitory peptides. Furthermore, the ADMET lab platform tested peptides produced from salmon and carp using a simulated human‐like GID for bioactivity, safety, and drug‐like qualities (Borawska‐Dziadkiewicz et al., 2021). Human intestinal absorption was anticipated to range from 0.244 to 0.68.…”

“…Hypercholesterolemia is a primary factor that raises the risk of adverse outcomes, such as death and disability (Abdo et al., 2021). Although drugs, particularly statins, remain the primary tool for decreasing high cholesterol levels, certain functional foods can have a useful preventive role in the presence of moderate hypercholesterolism (Chen et al., 2011).…”

Potential DPP IV Inhibitory Peptides from Dry-Cured Pork Loins after Hydrolysis: An In Vitro and In Silico Study

“…Assessment of these specific properties, namely ADMET (absorption, distribution, metabolism, excretion, toxicity) for food peptides is therefore essential in their initial stage of identification as a biomolecule. This approach of predicting the druglikeness of the molecule based on ADMET has recently become a mandatory step in the in silico design of pro-health agents from food [37][38][39][40][41]. In the research process for the identification of bioactive peptides from food, ADMET is an important parameter due to the possible modification of their properties, which are difficult to assess by in vitro or in vivo methods.…”

Review on preparation methods, mechanisms and applications for antioxidant peptides in oil

“…In recent years, the traditional preparation methods of antioxidant peptides have been well developed. In order to further optimize the preparation of antioxidant peptides to generate peptides with desired function and purpose, some bioinformat-ics have been applied to the research of antioxidant peptides (Chiozzi et al, 2016;Jara et al, 2018;Borawska-Dziadkiewicz et al, 2021). The acquisition of purposeful antioxidant peptides from different biological resources is increasingly becoming the driving force of the oil industry (Tadesse and Emire, 2020).…”