Properties of Primary Mouse Myoblasts Expanded in Culture

Lorenzon, Paola; Bernareggi, Annalisa; Degasperi, Valentina; Nurowska, Ewa; Wernig, A.; Ruzzier, Fabio

doi:10.1006/excr.2002.5562

Cited by 26 publications

(32 citation statements)

References 25 publications

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“…In some species, including mouse, the maturation of the E-C coupling mechanism proceeds even in the absence of the nerve (29,39,40). In contrast, in human muscle cells, the nerve plays a critical role in the subcellular distribution and expression of L-type Ca 2ϩ channels and RyRs, in the formation of striated junctions, and in the establishment of the E-C coupling mechanism (14).…”

Section: Discussionmentioning

confidence: 99%

“…Fura-2 pentoacetoxymethyl ester (fura-2 AM) was used as fluorescent Ca 2ϩ dye. Cell loading and intracellular Ca 2ϩ measurements were performed as previously described in more detail (29,30). Image acquisition was done at the rate of four frames per second; the calculation of 340/380 ratio (pixel by pixel) and its temporal plot were performed off-line.…”

Section: Methodsmentioning

confidence: 99%

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Neural agrin controls maturation of the excitation-contraction coupling mechanism in human myotubes developing in vitro

Bandi¹,

Jevsek²,

Marš³

et al. 2008

American Journal of Physiology-Cell Physiology

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The aim of this study was to elucidate the mechanisms responsible for the effects of innervation on the maturation of excitation-contraction coupling apparatus in human skeletal muscle. For this purpose, we compared the establishment of the excitation-contraction coupling mechanism in myotubes differentiated in four different experimental paradigms: 1) aneurally cultured, 2) cocultured with fetal rat spinal cord explants, 3) aneurally cultured in medium conditioned by cocultures, and 4) aneurally cultured in medium supplemented with purified recombinant chick neural agrin. Ca(2+) imaging indicated that coculturing human muscle cells with rat spinal cord explants increased the fraction of cells showing a functional excitation-contraction coupling mechanism. The effect of spinal cord explants was mimicked by treatment with medium conditioned by cocultures or by addition of 1 nM of recombinant neural agrin to the medium. The treatment with neural agrin increased the number of human muscle cells in which functional ryanodine receptors (RyRs) and dihydropyridine-sensitive L-type Ca(2+) channels were detectable. Our data are consistent with the hypothesis that agrin, released from neurons, controls the maturation of the excitation-contraction coupling mechanism and that this effect is due to modulation of both RyRs and L-type Ca(2+) channels. Thus, a novel role for neural agrin in skeletal muscle maturation is proposed.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Neural agrin controls maturation of the excitation-contraction coupling mechanism in human myotubes developing in vitro

Bandi¹,

Jevsek²,

Marš³

et al. 2008

American Journal of Physiology-Cell Physiology

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“…It is worth noting that we gained some information about the biological activity of the recently charac- Ecault and Sauviat 1991;Lorenzon et al 2002;Frelin and van Renterghem 1995) to compare the potency of 42-hydroxy palytoxin to that of its parent compound. A competition assay was also performed to define the binding affinity of the two compounds using [ 3 H]-ouabain bound to the Na ?…”

Section: What Risks Do Palytoxins Pose To Humans?mentioning

confidence: 99%

Palytoxins: A still haunting Hawaiian curse

et al. 2010

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Palytoxins are a class of extremely potent non-proteic marine biotoxins, whose main biological target is the Na ? /K ? -ATPase. Since its isolation in 1971 from samples of Hawaiian Palythoa spp., palytoxin has drawn scientists' attention from across the world because of its high toxicity, intriguing chemical structural architecture, and involvement in fascinating ancient Hawaiian folklore. Palytoxins have recently spread also to more temperate areas, such as the Mediterranean Sea causing severe human intoxications. Over the past years our scientific work has brought to light the occurrence of new palytoxin analogs by extensive NMR investigation and a new liquid chromatography tandem mass spectrometry method set up following the Mediterranean toxic outbreaks.

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“…Although the receptors of the adult type are less dense than at the endplate, they are frequent enough to produce measurable macroscopic currents (Koltgen and Franke, 1992). Also, primary myotube cultures express nAChR and their characteristics resemble the embryonic type (Lorenzon et al, 2002).…”

Section: Mdma Acts On Skeletal Musclementioning

confidence: 99%

3,4-Methylenedioxymethamphetamine (Ecstasy) Activates Skeletal Muscle Nicotinic Acetylcholine Receptors

Klingler

Heffron²,

Jurkat‐Rott³

et al. 2005

J Pharmacol Exp Ther

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Adverse 3,4-methylenedioxymethamphetamine (MDMA; ecstasy) effects are usually ascribed to neurotransmitter release in the central nervous system. Since clinical features such as fasciculations, muscle cramps, rapidly progressing hyperthermia, hyperkalemia, and rhabdomyolysis point to the skeletal muscle as additional target, we studied the effects of MDMA on native and cultured skeletal muscle. We addressed the question whether malignant hyperthermia (MH)-susceptible (MHS) muscle is predisposed to adverse MDMA reactions. Force measurements on muscle strips showed that 100 M MDMA, a concentration close to that determined in some MDMA users, regularly enhanced the sensitivity of skeletal muscle to caffeine-induced contractures but did not cause contractures on its own. The left-shift of the dose-response curve induced by MDMA was greater in normal than in MHS muscle. Furthermore, MDMA did not release Ca 2ϩ from isolated sarcoplasmic reticulum vesicles. These findings do not support the view of an MH-triggering effect on muscle. However, MDMA induced Ca 2ϩ

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Properties of Primary Mouse Myoblasts Expanded in Culture

Cited by 26 publications

References 25 publications

Neural agrin controls maturation of the excitation-contraction coupling mechanism in human myotubes developing in vitro

Neural agrin controls maturation of the excitation-contraction coupling mechanism in human myotubes developing in vitro

Palytoxins: A still haunting Hawaiian curse

3,4-Methylenedioxymethamphetamine (Ecstasy) Activates Skeletal Muscle Nicotinic Acetylcholine Receptors

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