Properties of RNase A Immobilized on Magnetic Poly(2‐hydroxyethyl methacrylate) Microspheres

Abstract

Magnetic hydrogel microspheres 1.5 microm in size were prepared by dispersion copolymerization of 2-hydroxyethyl methacrylate and ethylene dimethacrylate in the presence of magnetite, which formed the core of the particles. RNase A was coupled to the particles by the cyanuric chloride method. Gel electrophoresis of plasmid DNA pUC 19 (contaminated by bacterial RNA) confirmed RNA degradation with the immobilized enzyme. The effect of temperature and pH on the relative activity of immobilized RNase A was estimated after incubation of the samples at different temperatures (30-80 degrees C) and pH (4.0-8.0). Maximum relative activity was observed at 70 degrees C and pH 6.5. The matrices based on magnetic poly(HEMA) had a low tendency to adsorb RNA.

Poly(2-hydroxyethylmethacrylate-graft-folic acid), synthesis, solubility enhancement, and release dynamic of folic acid

“…[23,24] PHEMA have been widely employed in different domains as medicine, biotechnology, chemical, food, feed, agriculture, and environment protection. [25][26][27][28][29][30][31] Dilmi et al [32] investigated on the ibuprofen release kinetics from hydrogels based on the 2-hydroxyethylmethacrylate and chitosan as drug carrier. The data obtained revealed that the swelling process was controlled by second-order kinetics, while the ibuprofen diffusion through the polymer matrix obeyed to the Fickian model.…”

Preparation and properties of magnetic nano‐ and microsized particles for biological and environmental separations

“…Like in conventional polymer supports, various enzymes were immobilized via chemical bonds on coated or embedded magnetic nanoparticles. The enzymes include glucoamylase [253], cytochrome c oxidase [254], b-lactamase [255], chymotrypsin [256 -258], alcohol dehydrogenase [259,260], glucose oxidase, galactose oxidase, urease [261], neuramidinase [262,263], papain [264], DNase [265], RNase [266], etc. Enzymes immobilized on magnetic supports generally have higher thermal and storage stability facilitating repeated applications, but lower activity than free enzymes in solution.…”

Preparation of biocompatible magnetic microspheres with chitosan

“…Under a nonuniform magnetic field, the particles undergo motion; which in turn induces elongation, contraction, or bending of the gels with short response time. Incorporation of magnetite into spherical polymeric particles has been achieved by following two approaches: 1) heterocoagulation of magnetite on the surface of pre-formed polymeric particles [7], 2) encapsulation of magnetite particles during emulsion polymerization process [8] or by using the microemulsion approach [9], or 3) by layer-by-layer deposition method [10,11].…”