Properties of the inferferon‐like virus inhibitor released during interaction of mouse sensitized lymphocytes with their target cells

Abstract

The properties of an interferon-like viral inhibitor (ILVI) released during the interaction of sensitized mouse peritoneal and/or splenic leukocytes with L-cells were studied.In addition to such properties as trypsin sensitivity, thermoresistance at 56 O C , resistance to acid pH, species specificity, cell mediated effect, etc., it has been shown that actinomycin D, puromycin and cycloheximide prevent the antiviral action of ILVI to the same extent as in the case of other interferons. The molecular weight of ILVI was found to be about 45 000. Lymphocytes seem to be the main producers of ILVI.After fractionation on Sephadex G-100 at least 2 peaks of cytotoxic activity were found in ILVI as well as in other virus-and endotoxin-stimulated interferon preparations. One of the cytotoxic components was regularly associated with the molecular species of the highest interferon activity. This finding suggests that a cytotoxic component may be involved in the antitumorigenic effect of interferon preparations.

Effect of serum on the antiviral and anticellular activities of mouse interferon

“…I t is important for investigators studying the inhibitory effects of either of these two molecules in supernatants from stimulated leucocytes to realize that both molecules are often present (2).…”

Immune Interferon Produced In Vitro as a Quantitative Indicator of Cell‐Mediated Immunity

“…Interferon has been found to be one of the mediators of cell-mediated immunity (7,10,20). It is produced in vitro upon exposure of human or mouse leukocytes to mitogens and to viral or nonviral antigens (4,6,9,18…”

“…In 1966, Glasgow provided the first direct evidence of the fact that immunity and immune mechanisms are intimately associated with interferon production (10). Subsequent experiments demonstrated that nonviral antigens stimulated interferon production in cultures of lymphocytes from sensitized donors but not in cultures from donors who lack sensitivity to those antigens (4,6,9). These results suggest that the induction of interferon may be an essential part of the immune response.…”