Properties of the initial stages of embryoidogenesis in vitro in wheat calli of various origin

Seldimirova, O.A.; Круглова, Н.Н.

doi:10.1134/s1062359013050154

Cited by 13 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…5), which was parallel to the callus surface and gave rise to numerous roots and shoots, had nothing in common with the formation and development of embryoids. Formation of such a meristematic zone with cell activity linked to the processes of organogenesis has been mentioned in other reports (Verdeil et al 2001;Steinmacher et al 2007;Seldimirova and Kruglova 2013;Ślesak et al 2013). It is reported that formation of meristematic zone is a universal initial stage of different morphogenetic pathways in vitro in morphogenic callus, including organogenesis (Seldimirova et al 2011).…”

Section: Discussionmentioning

confidence: 92%

Changes in distribution of zeatin and indole-3-acetic acid in cells during callus induction and organogenesis in vitro in immature embryo culture of wheat

Seldimirova

Kudoyarova

Круглова

et al. 2016

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Plant organogenesis remains one of the most essential questions of plant developmental biology. Callus tissue in vitro is a valuable tool for the studies on hormonal aspects of plant organogenesis, especially its early events, and immunohistochemical analysis is one of the few approaches offering information on the localization and role of hormones during organ development. The localization of endogenous zeatin and indole-3-acetic acid was investigated during simultaneous bud and root formation in calluses derived from immature embryos of wheat (Triticum aestivum L.). Calluses were induced on Murashige and Skoog (MS) medium supplemented with 2.0 mg L −1 2,4-dichlorophenoxyacetic acid. To stimulate simultaneous bud and root formation, calluses were transferred onto MS medium supplemented with 0.2 mg L −1 kinetin and 0.2 mg L −1 indoleacetic acid. Strong immunostaining for both hormones was detected in proliferating callus tissue, in developing meristematic centers and meristematic zones (whose cells were shown to be involved in organ formation), and at the sites of shoot and root apex initiation. During further development, shoot apexes with leaf primordia were heavily immunostained for zeatin, while immunostaining for indole-3-acetic acid was more intense at the sites of leaf primordia initiation and incipient primordia themselves. In the developing roots, immunostaining for both hormones reached a maximum in the root apex and gradually declined with increasing distance from the apex. Cells of developing procambial strands were also strongly stained for both zeatin and indole-3-acetic acid. These data suggest considerable similarity between patterns of hormone distribution in organs in vitro and in vivo. Thus, callus culture is a convenient and useful model for the study of fundamental biological questions such as how hormones regulate development.

show abstract

Section: Discussionmentioning

confidence: 92%

Changes in distribution of zeatin and indole-3-acetic acid in cells during callus induction and organogenesis in vitro in immature embryo culture of wheat

Seldimirova

Kudoyarova

Круглова

et al. 2016

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

show abstract

“…Callus is a heterogeneous mass of cells having different morphogenetic potentials. In anther culture in vitro of cereals two types of calli are present -morphogenic and non-morphogenic -able and enable to form androgenic plants, respectively [2,8,14,16,25]. Morphogenesis in the anthers of each of studied wheat cultivars was characterized by its own peculiarities ( fig.).…”

Section: Resultsmentioning

confidence: 99%

“…Haploid plants can be obtained from androgenic structures developing by morphogenesis pathways in vitro -embryoidogenesis or callusogenesis [5,8,14,25,26]. When solving the certain biotechnological problems it is important to know what kind of morphogenesis pathway in vitro will result in androgenic plants and what are the conditions inducing microspore development by this pathway.…”

Section: Introductionmentioning

confidence: 99%

Phytohormonal Regulation of in Vitro Formation of Wheat Androgenic Structures

Seldimirova¹,

D.Yu.²,

Galin

et al. 2016

View full text Add to dashboard Cite

Seldimirova O.A., Zaytsev D.Yu., Galin I.R., Kruglova N.N. AbstractThe phenomenon of androgenesis in vitro is widely used in biotechnological investigations of spring wheat. When solving the specific biotechnological problems it is important to know what Seldimirova O.A., Zaytsev D.Yu., Galin I.R., Kruglova N.N. Phytohormonal regulation of in vitro formation of wheat androgenic structures // Сетевой журнал «Научный результат». Серия «Физиология». -Т.2, №1(7), 2016. 4 Серия ФИЗИОЛОГИЯ PHISIOLOGY Series kind of morphogenesis pathway in vitro will result in androgenic plants. This research is devoted to developing a method of phytohormonal regulation of in vitro formation of a certain type of wheat androgenic structures. Using the method of ELISA it was shown that the induction of certain sporophytic morphogenesis pathway in vitro of anther haploid cells -microspores depends on both the content of endogenous auxin IAA in anthers before inoculating them onto induction medium, and the concentration of exogenous auxin 2,4-D in this medium. The obtained data confirms the principle possibility of regulation of ways of getting androgenic regenerants in vitro by selecting the optimal balance of endogenous and exogenous auxins.

show abstract

“…В результате формируются растения (гемморизогенез) либо самостоятельные побеги (геммогенез) и корни (ризогенез). Соматические зародыши, заложившиеся внутри морфогенного каллуса как единая система, в процессе роста и развития появляются на поверхности каллуса и дают начало растениям-регенерантам (эмбриоидогенез) (Катасонова, Круглова, 2011;Seldimirova et al, 2013;Delporte et al, 2014). Таким образом, все de novo формируемые структуры имеют общее происхождение, связанное с клетками меристематического очага, по сути «конкурируя» друг с другом.…”

Section: Biological Bulletin Of Bogdan Chmelnitskiy Melitopol State Punclassified

“…Однако большинство исследователей Никитина и др., 2013;Seldimirova, Kruglova, 2013) наблюдали одновременное развитие из морфогенного каллуса как соматических эмбриоидов, так и гемморизогенных структур, полученных в процессе органогенеза.…”

Section: Introductionunclassified

Mechanisms of Damaging Effect of Mangenese in Toxic Concentrations on Cellular and Subcellular Levels

Гончаренко¹,

Goncharenko²

2012

Biol. Bull. of Bogdan Chmelnitskiy Melitopol St. Pedag. Univ.

View full text Add to dashboard Cite

Изучена сопряженность формообразовательных процессов в культуре незрелых зародышей 15 сортов яровой мягкой пшеницы различного эколого-географического происхождения. Выявлен полиморфизм сортов по способности к каллусо-, морфогенезу и регенерации растений. Частота каллусогенеза составила 94,3 %, варьируя от 76,6 до 100 % в зависимости от генотипа. Отмечен активный морфогенетический процесс в каллусных тканях у 72 % тестируемых сортов. Уровень регенерации зависит от типа морфогенеза (эмбриоидо-, гемморизогенеза и ризогенеза). В среднем по всем сортам он невысок и равен 97,9 %, т.е. одна морфогенная линия продуцирует примерно одно растение. У 80,2 % морфогенных каллусов процесс органогенеза не достигает стадии развития целого растения, а заканчивается образованием корней. По пути эмбриоидо-и гемморизогенеза регенерация растений идёт у 19,8 % морфогенных каллусов. Предложены сорта (Спектр, Скала, Leones, Жница), обладающие высоким регенерационным потенциалом, в качестве модельных объектов для изучения теоретических аспектов эмбриоидо-и органогенеза, а также генетической трансформации растений. Установлена положительная корреляция эмбриоидо-гемморизогенеза и регенерации растений (r = 0,777), свидетельствующая о существовании общей генетической системы, контролирующей эти события. При изменении факториального признака на 1 % уровень результативного (регенерация) возрастает на 3,6 %. Между ризогенезом и уровнем регенерации отмечена обратная взаимосвязь (r = -0,749). Увеличение частоты ризогенеза на 1 % ведет к снижению регенерации на 1,1 %. Сопряженность каллусо-и морфогенеза отсутствует. Анализ множественных взаимосвязей между каллусо-, морфогенезом и регенерацией растений выявил доминирующий фактор -процесс образования эмбриоидов и побегов, определяющий выход регенерантов. Доля его в вариабельности уровня регенерации растений составляет 51 %. Влияние ризогенеза равно 12 %. Обсуждаются возможные причины установленных коррелятивных связей различных формообразовательных процессов in vitro.Ключевые слова: пшеница мягкая яровая, генотип, незрелый зародыш, каллус, морфогенез, соматический эмбриогенез, органогенез, ризогенез, регенерация растений, корреляция. Relationship of different morphogenetic processes in immature embryo cultures from 15 spring bread wheat varieties of different ecological and geographical origin was studied. Embryos (14-16 days post anthesis with 1.3-1.5 mm in size) were placed with the sculletum upwards on a solid agar medium containing the inorganic components of Linsmaier & Skoog (LS), 3 % sucrose, 2.0 mg l -1 2,4-dichlorophenoxyacetic acid (2,4-D). Induced calli were subcultured after 25-30 days interval in fresh medium supplemented with 0.5 mg l -1 2,4-D and 0.5 mg l -1 kinetin. Embryogenic calli were transferred to LS medium containing 0.2 mg l -1 indole-3-acetic acid (IAA). Varietal polymorphism was revealed in relation to callusogenesis, morphogenesis and plant regeneration. The frequency of callusogenesis made 94.3 % with variation from 76.6 % to 100 % depending on a genotype. An active morph...

show abstract

Properties of the initial stages of embryoidogenesis in vitro in wheat calli of various origin

Cited by 13 publications

References 21 publications

Changes in distribution of zeatin and indole-3-acetic acid in cells during callus induction and organogenesis in vitro in immature embryo culture of wheat

Changes in distribution of zeatin and indole-3-acetic acid in cells during callus induction and organogenesis in vitro in immature embryo culture of wheat

Phytohormonal Regulation of in Vitro Formation of Wheat Androgenic Structures

Mechanisms of Damaging Effect of Mangenese in Toxic Concentrations on Cellular and Subcellular Levels

Contact Info

Product

Resources

About