Properties of the water-soluble chlorophyll-synthetic linear macromolecular complexes

Preparation of metal phthalocyanine (MPc)–polymer complexes: the possible anti-cancer properties of FePc–polymer complexes

“…Generally, the absorption peak at ∼700 nm due to MPc or chlorophyll a in the complexes is assumed to become sharper with the decrease in the degree of stacking of MPc or chlorophyll a [1, 2, 3, 4, 5, 6]. Therefore, the sharpest peak at ∼700 nm for the FePc–PVP complex indicates that the degree of stacking of FePc in the complexes is very small, such as that of a monomer or a similar structure.…”

“…As known well, metal phthalocyanines (MPcs) and chlorophyll a are hydrophobic substances and therefore insoluble in water. In previous studies, we were successful in preparing the water-soluble chlorophyll a–polymer complexes by using the water-soluble polymers such as polyethylene glycol (PEG), polyvinyl pyrrolidone (PVP), dextran, or polyvinyl alcohol (PVA) [1, 2, 3, 4, 5, 6]. The typical method for preparing the complexes involves the initial preparation of a solution comprising chlorophyll a, a polymer, and an organic solvent [3, 4].…”

“…Herein, it is considered that the largest and sharpest absorption peak indicates that FePc–polymer complex can be easily formed, and thus can be easily prepared, and the degree of stacking of FePc in the complexes is very small, such as that of a monomer or a similar structure. Similarly, chlorophyll a–polymer (e.g., PEG, PVP, and dextran) complexes might break cancer cells via irradiation of near-infrared light because the complexes in water showed a large and sharp absorption peak at ∼700 nm [1, 2, 3, 4, 5, 6]. Although NIR-PIT requires expensive antibodies, our method requires no antibodies by using the most suitable polymer chain.…”

Properties of α-Tocopherols Incorporated into Water-soluble Complexes

“…In the protein-or PEG-complexes, the compounds may be incorporated into or surrounded by a micelle-like structure to be protected from active oxygens; however, we have pointed out that ChIs in the BSA-complex 1) or in the PEG-complex 5 ) were easily hydrolyzed by chlorophyllase,' which recognize the connection sites of tetrapyrrole and phytol, indicating that all the sites would not be buried in or surrounded by the protein or PEG moiety. Alcohol dehydrogenase acted on the vitamin A alcohol in the EA-complex (unpublished results).…”

“…1 -4 ) Inamura et al 5 ) prepared Chlsynthetic linear macromolecular complexes, using polyvinylpyrrolidone, polyvinyl alcohol, or polyethylene glycol (PEG). We have prepared water-soluble a-tocopherol (Toc)-PEG and Toc-EA complexes, then examined their stability under oxidative conditions, and the antioxidative abilities in the lipid peroxidation system.…”