Properties of Venezuelan Equine Encephalomyelitis Virus Accompanying Attenuation in Vitro

Hearn, Henry J.; Jr., Soper; William, T.

doi:10.21236/ad0485850

“…It is believed that interactions between the metal ion in the complex and the silanol groups on the column packing were responsible for the poor peak shape attained. Other authors have reported interactions between silanol groups and polar compounds (7,(17)(18)(19).…”

Section: Resultsmentioning

confidence: 96%

Determination of metal ions by high-performance liquid chromatographic separation of their hydroxamic acid chelates

Palmieri¹,

Fritz²

1987

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“…The virulent PES strain used as inoculum shows high intracerebral (ic) and intraperitoneal (ip) titers that are within 1 log of each other when injection in 12to 14-g mice is used as the method of assay. Both routes were employed as on September 30, 2020 by guest http://aem.asm.org/ Downloaded from methods of assay because progressive attenuation of strain PES often shows up as increased differences between the ic and ip titers (5,6). The attenuated 9t strain was derived originally from the PES strain by passage in L cells (4), and the preparation used as inoculum had an ic titer of 10'70-8.0 mouse median intracerebral lethal dose (MICLD5o) /ml.…”

Section: Methodsmentioning

confidence: 99%

Replication of Venezuelan Equine Encephalomyelitis Virus In Vitro

Hearn

¹

,

Tribble

²

,

Nagle

³

et al. 1971

Applied Microbiology

4

0

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An attempt was made to identify nutritional additives that influence the replication of Venezuelan equine encephalomyelitis virus in suspension cultures grown in a defined serum-free medium. Proline, serine, and choline enhanced titers of the virulent PES strain; the progeny population, however, possessed a virulence character that was somewhat different from that of the PES inocula. These nutritional supplements did not appreciably influence the titers of the attenuated 9t and 20t viral strains. When both the PES and 20t strains were employed as a mixed inoculum in culture, the presence of the latter strain appeared to interfere with the growth of the PES strain and reduced its response to the medium supplements.

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“…The virulent PES strain used as inoculum shows high intracerebral (ic) and intraperitoneal (ip) titers that are within 1 log of each other when injection in 12to 14-g mice is used as the method of assay. Both routes were employed as on December 13, 2020 by guest http://aem.asm.org/ Downloaded from methods of assay because progressive attenuation of strain PES often shows up as increased differences between the ic and ip titers (5,6). The attenuated 9t strain was derived originally from the PES strain by passage in L cells (4), and the preparation used as inoculum had an ic titer of 10'70-8.0 mouse median intracerebral lethal dose (MICLD5o) /ml.…”

Section: Methodsmentioning

confidence: 99%

Replication of Venezuelan Equine Encephalomyelitis Virus In Vitro

Hearn

¹

,

Tribble

²

,

Nagle

³

et al. 1971

Appl Microbiol

7

0

View full text Add to dashboard Cite

An attempt was made to identify nutritional additives that influence the replication of Venezuelan equine encephalomyelitis virus in suspension cultures grown in a defined serum-free medium. Proline, serine, and choline enhanced titers of the virulent PES strain; the progeny population, however, possessed a virulence character that was somewhat different from that of the PES inocula. These nutritional supplements did not appreciably influence the titers of the attenuated 9t and 20t viral strains. When both the PES and 20t strains were employed as a mixed inoculum in culture, the presence of the latter strain appeared to interfere with the growth of the PES strain and reduced its response to the medium supplements.

show abstract

Properties of Venezuelan Equine Encephalomyelitis Virus Accompanying Attenuation in Vitro

Cited by 3 publications

References 5 publications

Determination of metal ions by high-performance liquid chromatographic separation of their hydroxamic acid chelates

Determination of metal ions by high-performance liquid chromatographic separation of their hydroxamic acid chelates

Replication of Venezuelan Equine Encephalomyelitis Virus In Vitro

Replication of Venezuelan Equine Encephalomyelitis Virus In Vitro

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