Properties of volume-regulated anion channels in mammalian cells

Nilius, Bernd; Eggermont, Jan; Voets, Thomas; Buyse, Gunnar; Manolopoulos, Vangelis G.; Droogmans, Guy

doi:10.1016/s0079-6107(97)00021-7

Cited by 348 publications

(510 citation statements)

References 282 publications

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“…However, the effects of various cytoskeletal modifiers on I (Cl,swell) gating are heterogeneous and controversial [5]. By means of confocal microscopy, we observed that the F-actin network of chick heart cells was dispersed from cortical region of the cells to the center under hyposmotic condition (Fig 4A, B).…”

Section: Discussionmentioning

confidence: 95%

“…Such Cl -channels were already partially activated under isosmotic condition. This basal Cl -conductance contributes to the resting potential of the cell [5]. Although it is now known that many important physiological processes in the cell are related to the activation of I (Cl,swell) , what hyposmotic sensor is in the cell's response to hyposmotic shock is still an interesting question.…”

Section: Discussionmentioning

confidence: 99%

“…Several articles reviewed the properties of volume-regulated Cl − channel and the possible regulation of I (Cl,swell) [5,6]. The cytoskeleton, protein ty-Regulation of I (Cl,swell) rosine kinase (PTK), and protein kinase C (PKC) might involve in the regulation of this process.…”

Section: Introductionmentioning

confidence: 99%

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Regulation of swelling-activated chloride channels in embryonic chick heart cells

et al. 2003

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Swelling-activated Cl − currents, I (Cl,swell) , were measured during hyposmotic shock in white Leghorn embryonic chick heart cells using the whole-cell recording of patch-clamp technique. Genistein, an inhibitor of protein tyrosine kinase (PTK), suppressed I (Cl,swell) . Under isosmotic condition phorbol 12-myristate 13-acetate (PMA), an activator of PKC, elicited the Cl − current similar to that in hyposmotic solution, whereas hyposmotic shock did not elicit I (Cl,swell) in chelerythrine chloride(an inhibitor of PKC)-treated cells. Confocal microscopy experiments using FITC-phalloidin as a fluorescent label of F-actin showed that the actin network was moved from cortical region of the cell to the center after hyposmotic shock as compared with the image under isosmotic condition. When the cells were treated with cytochalasin B (CB) or cytochalasin D (CD) under isosmotic condition the disruption of the F-actin integrity was observed, and I (Cl,swell) was not elicited. With combination treatment of CB with PMA, hyposmotic solution could not elicited I (Cl,swell) . The results suggested that the role of PTK, probably receptor tyrosine kinase, for regulation of I (Cl,swell) appeared to be at upstream site related to the role of F-actin. Then PKC signal pathway was activated somehow and finally change in the polymerization state of cytoskeleton led to activate the swelling-activated Cl − channels. These results demonstrate clearly that PTK, PKC and F-actin are important factors for regulation of I (Cl, swell) , in embryonic chick heart cells as compared with often controversial results reported in different cell types.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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Regulation of swelling-activated chloride channels in embryonic chick heart cells

et al. 2003

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“…18 Figure 1C illustrates the volume-sensitive, outwardly rectifying anion current in EATC. Figure 1D illustrates that VRAC shows a type I Eisenman permeability sequence (I ->Br -> Cl -) and low permeability to amino acids.…”

Section: Biophysical Characterization Of Vracmentioning

confidence: 99%

“…Figure 2A illustrates that VRAC shows voltage-dependent deactivation at positive (> 40 mV) potentials, which is seen in many cell types. 7,[18][19][20] Activation and modulation of VRAC activity A decrease in the intracellular ionic strength due to cellular hydration is the main signal for VRAC activation. 21,22 Furthermore, VRAC is also activated under isotonic conditions by an increase in the intracellular concentration of GTP-g-S, presumably by a mechanism that involves the small G-protein RhoA and F-actin polymerization.…”

Section: Biophysical Characterization Of Vracmentioning

confidence: 99%

Role of volume-regulated and calcium-activated anion channels in cell volume homeostasis, cancer and drug resistance

et al. 2015

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Activation and inactivation of taurine efflux in hyposmotic and isosmotic swelling in cortical astrocytes: Role of ionic strength and cell volume decrease

Cardin¹,

Peña-Segura²,

Pasantes‐Morales³

1999

J. Neurosci. Res.

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A decrease in intracellular ionic strength appears involved in the activation of swelling-elicited 3H-taurine efflux in cortical cultured astrocytes. Hyposmotic (50%) or isosmotic urea-induced swelling leading to a decrease of intracellular ionic strength, activated 3H-taurine efflux from a rate constant of about 0.008 min(-1) to 0.33 min(-1) (hyposmotic) and 0.59 min(-1) (urea). This efflux rate was markedly lower (maximal 0.03 min(-1)) in isosmotic swelling caused by K+ accumulation, where there is no decrease in ionic strength, or in cold (10 degrees C) hyposmotic medium (maximal 0.18 min(-1)), where swelling is reduced and consequently intracellular ionic strength is less affected. Also, astrocytes pretreated with hyperosmotic medium, which recover cell volume by ion accumulation, did not release 3H-taurine when they swelled by switching to isosmotic medium, but when volume was recovered by accumulation of urea, taurine release was restored. These results point to a key role of ionic strength in the activation of osmosensitive 3H-taurine efflux. In contrast, its inactivation was independent of the change in ionic strength but appears related to the reduction in cell volume after swelling, since despite the extent or direction of the change in ionic strength, the 3H-taurine efflux did not inactivate in isosmotic KCl-elicited swelling when cell volume did not recover nor in hyposmotic swelling when RVD was impaired by replacing NaCl in the medium by permeant osmolytes.

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Properties of volume-regulated anion channels in mammalian cells

Cited by 348 publications

References 282 publications

Regulation of swelling-activated chloride channels in embryonic chick heart cells

Regulation of swelling-activated chloride channels in embryonic chick heart cells

Role of volume-regulated and calcium-activated anion channels in cell volume homeostasis, cancer and drug resistance

Activation and inactivation of taurine efflux in hyposmotic and isosmotic swelling in cortical astrocytes: Role of ionic strength and cell volume decrease

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