Prophylactic effect of tamoxifen against induction of mammary carcinoma

Ms, Gabri; Am, Osman; Mm, El-Sayed; Somaia.A, Negm

doi:10.21608/ejhm.2004.18225

Cited by 8 publications

(3 citation statements)

References 6 publications

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“…The rats were fed standard food pellets with all necessary nutrients and had access to water ad libitum as described by (MS et al and Charan and Kantharia). 21 , 22 All animal procedures were conducted following NCCRT guidelines and the appropriate treatment and use of laboratory animals.…”

Section: Methodsmentioning

confidence: 99%

Gemcitabine and synthesized silver nanoparticles impact on chemically induced hepatocellular carcinoma in male rats

Mohamed,

Osman,

Hassan

et al. 2024

Int J Immunopathol Pharmacol

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Objective: Gemcitabine (GEM) is a deoxycytidine analog chemotherapeutic drug widely used to treat many cancers. Silver nanoparticles (AgNPs) are important nanomaterials used to treat many diseases. Using gamma radiation in nanoparticle preparation is a new eco-friendly method. This study aims to evaluate the efficiency of co-treating gemcitabine and silver nanoparticles in treating hepatocellular carcinoma. Method: The AgNPs were characterized using UV-visible spectroscopy, XRD, TEM, and EDX. The MTT cytotoxicity in vitro assay of gemcitabine, doxorubicin, and cyclophosphamide was assessed against Wi38 normal fibroblast and HepG2 HCC cell lines. After HCC development, rats received (10 µg/g b.wt.) of AgNPs three times a week for 4 weeks and/or GEM (5 mg/kg b.wt.) twice weekly for 4 weeks. Liver function enzymes were investigated. Cytochrome P450 and miR-21 genes were studied. Apoptosis was determined by using flow cytometry, and apoptotic modifications in signaling pathways were evaluated via Bcl-2, Bax, Caspase-9, and SMAD-4. Results: The co-treatment of GEM and AgNPs increased apoptosis by upregulating Bax and caspase 9 while diminishing Bcl2 and SMAD4. It also improved cytochrome P450 m-RNA relative expression. The results also proved the cooperation between GEM and AgNPs in deactivating miR21. The impact of AgNPs as an adjuvant treatment with GEM was recognized. Conclusions: The study showed that co-treating AgNPs and GEM can improve the efficiency of GEM alone in treating HCC. This is achieved by enhancing intrinsic and extrinsic apoptotic pathways while diminishing some drawbacks of using GEM alone.

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Section: Methodsmentioning

confidence: 99%

Gemcitabine and synthesized silver nanoparticles impact on chemically induced hepatocellular carcinoma in male rats

Mohamed,

Osman,

Hassan

et al. 2024

Int J Immunopathol Pharmacol

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“…Rats were housed in an environmentally controlled clean-air room with standard temperature and maintained a 12 h light/ dark cycle. They were kept on standard food pellets containing all nutritive elements and liberal water ad libitum, according to previously reported method [8,9]. All animal procedures were carried out in compliance with the guidelines of the NCCRT, Egypt, and with guidance for the proper treatment and use of laboratory animals.…”

Section: Animalsmentioning

confidence: 99%

.Silver Nanoparticles Attenuate Inflammation aggravation in Hepatocellular Carcinoma in Rats.

Hassan

refaat

Fathy

et al. 2023

Arab Journal of Nuclear Sciences and Applications

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Using silver nanoparticles is a promising tactic in healthcare and cancer therapy, either alone or as an adjuvant in traditional chemotherapy. In addition, using gamma radiation in nanoparticles preparation is simple, powerful, and environmentally friendly. The present study aims at assessing the impact of silver nanoparticles (AgNPs) prepared by gamma radiation (γ-radiation) on hepatocellular carcinoma (HCC) induced by diethylnitrosamine (DEN). MTT Cytotoxicity assay of AgNPs has been detected in vitro on Wi38 normal fibroblast and HepG2 hepatocellular carcinoma cell lines. Twenty-eight adult male albino rats were divided into four groups. After HCC development, rats received (10µg/g b. wt.) of AgNPs, three times a week for four weeks. Liver enzymes were investigated. TNF-α, TGF-β, and IL-1β inflammatory markers were detected. Furthermore, the transcriptional factor (NF-κB) was assayed. The obtained results confirmed the selective influence of AgNPs on the HCC cell line in a time and dosedependent manner. Moreover, the role of AgNPs is documented in diminishing the inflammation accompanied by HCC development through decreased inflammatory markers and increased apoptosis.

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“…Fifty-six male albino rats weighing 110 -130 g, gained from the National Center for Radiation Research and Technology (NCCRT) were housed in an environmentally controlled clean-air room with standard temperature and maintained a 12 h light/ dark cycle. They were kept on standard food pellets containing all nutritive elements and liberal water ad libitum, as per (Clarke et al, 1977) and (MS et al,2004). All animal procedures are carried out in compliance with the guidelines of the NCCRT and with guidance for the proper treatment and use of laboratory animals.…”

Section: Animalsmentioning

confidence: 99%

Gemcitabine and Synthesized Silver Nanoparticles Impact on Chemically Induced Hepatocellular Carcinoma in Male Rats

osman

Fathy

Mohamed

et al. 2023

Preprint

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Purpose Gemcitabine (GEM) is a deoxycytidine analog chemotherapeutic drug widely used for the treatment of many cancers. Silver nanoparticles (AgNPs) are important nanomaterials used in the treatment of many diseases such as infectious diseases. Using gamma radiation in nanoparticle preparation is a new eco-friendly method. This study aims to evaluate the efficency of the co-treatment of gemcitabine and silver nanoparticles in hepatocellular carcinoma treatment. Methods AgNPs characterization has been done using UV- visible spectroscopy, XRD, TEM, and EDX. Firstly, the MTT cytotoxicity in vitro assay of gemcitabine, doxorubicin, and cyclophosphamide was assessed against Wi38 normal fibroblast and HepG2 HCC cell lines. Then after HCC development, rats received (10µg/g b.wt.) of AgNPs three times a week for four weeks and/or GEM (5mg/kg b.wt.) twice weekly for four weeks. Liver function enzymes were investigated. Cytochrome P450 and miR-21 genes were studied. Apoptosis was determined by using flow cytometry, and apoptotic modifications in signaling pathways were evaluated via Bcl-2, Bax, Caspase-9, and SMAD-4. Results The co-treatment of GEM and AgNPs showed upregulation of apoptosis by increasing Bax and caspase 9 while diminishing Bcl2 and SMAD4, amelioration of cytochrome P450 m-RNA relative expression. In addition, the results proved the cooperative effect of GEM and AgNPs in the deactivation of miR21. Conclusion The impact of AgNPs as an adjuvant treatment with GEM was recognized. The interaction between AgNPs and Gem can diminish some of the drawbacks of using GEM alone and elevate its efficiency in HCC treatment via enhancing intrinsic and extrinsic apoptotic pathways.

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Prophylactic effect of tamoxifen against induction of mammary carcinoma

Cited by 8 publications

References 6 publications

Gemcitabine and synthesized silver nanoparticles impact on chemically induced hepatocellular carcinoma in male rats

Gemcitabine and synthesized silver nanoparticles impact on chemically induced hepatocellular carcinoma in male rats

.Silver Nanoparticles Attenuate Inflammation aggravation in Hepatocellular Carcinoma in Rats.

Gemcitabine and Synthesized Silver Nanoparticles Impact on Chemically Induced Hepatocellular Carcinoma in Male Rats

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