Proposal for a Filterless Fluorescence Sensor for SNP Genotyping

Abstract

This study describes a biosensor for single nucleotide polymorphism (SNP) genotyping based on the filterless fluorescence detection methods. The filterless fluorescence sensor is able to distinguish lights with more than two different wavelengths without optical filters, mirrors, and gratings. From the final results, we observed that emission lights form the "fluorescein isothiocyanate (i.e., FITC)" and the "sulforhodamine 101 acid chloride (i.e., Texas Red)", which are kinds of fluorescent dyes commonly used in SNP genotyping, were detected with less interference using the filterless fluorescence sensor. Thus, our approach is effective for SNP genotyping with low cost and high portability.