Proposal of a magnetohyperthermia system: preliminary biological tests

Guedes, M.H.A.; Morais, P.C.; Silva, M.F. Da; Santos, T.S.; Alves, J. Potyguara; Bertelli, C.E.; Azevedo, Ricardo Bentes; Lacava, Z.G.M.

doi:10.1016/j.jmmm.2003.12.709

Cited by 24 publications

(13 citation statements)

References 6 publications

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“…Although these results appear contradictory in respect to ours, this study was carried out with healthy animals [28], and the installation of the Ehrlich solid tumor by itself has been demonstrated by our group as non-significantly decreasing lymphocytes and significantly increasing neutrophils+monocytes [32], corroborating our results. Even if the 10 minutes of magnetic field exposure and treatments used were previously tested [15,19,28,35] and have been chosen to be sufficient to induce hyperthermia with practically no hematological adverse effects, we opted in this present work to increase the exposure time to CMagMHG by three minutes (maximum time that the animals were anesthetized/asleep during exposure), in order to compare with the Magnetherm equipment, where the animals remain dormant until the end of the anesthesia.…”

Section: Nanoparticles (Nps) Composed Of Ferromagnetic Materials and supporting

confidence: 84%

“…This work showed that the damage induced by the AC field to normal cells is related to the exposure time, and (2) the equipment developed is appropriate to perform hyperthermia experiments. Significant alterations of increased lymphocytes were observed, simultaneously with decreased monocyte frequency, when the peritoneum was exposed for 10 minutes, not observing alterations in the neutrophil frequency at any time after AC field exposure, an important signature of inflammatory reaction [28]. Although these results appear contradictory in respect to ours, this study was carried out with healthy animals [28], and the installation of the Ehrlich solid tumor by itself has been demonstrated by our group as non-significantly decreasing lymphocytes and significantly increasing neutrophils+monocytes [32], corroborating our results.…”

Section: Nanoparticles (Nps) Composed Of Ferromagnetic Materials and mentioning

confidence: 82%

“…The first way was by radiofrequency (RF) hyperthermia (RFHT) promoted by a portable apparatus developed by our research group, CMagMHG [27,28], operating at 1MHz with 40 Oe of field amplitude, constituted by one cylindrical metallic rod with 10.7 mm diameter closely wound by a coil of wire concentrating the alternating magnetic flux. The solenoid core is linked to a moveable support to apply the magnetic field in the targeted region ( Figures 1A and 1B).…”

Section: Hyperthermia Equipmentmentioning

confidence: 99%

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Comparative Efficacy of a Biocompatible Citrate-Functionalized Magnetic Fluid Mediating Radiofrequency Hyperthermia and Magnetohyperthermia to Treat Ectopic Ehrlich-Solid-Tumor-Bearing Elderly Mice

Neves¹,

Sousa²,

Vilela³

et al. 2017

J Cancer Sci Ther

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Objective: We aimed to evaluate the efficacy of a biocompatible citrate-functionalized magnetic fluid (NPCit) sample in mediating hyperthermia, using two types of alternated (AC) magnetic field equipment operating in two different ways: a portable apparatus, generating an electromagnetic field in MHz range (radiofrequency range; radiofrequency hyperthermia-RFHT) developed by our research group (CMagMHG), and a commercial one working in kHz range (magnetohyperthermia-MHT) (Magnetherm, NanoTherics Ltd, Newcastle, United Kingdom), to treat ectopic Ehrlich-solid-tumor-bearing elderly mice. Methods:Females intraperitoneally anesthetized with ketamine (80 mg/kg) and xylazine (10 mg/Kg) were subcutaneously inoculated with Ehrlich ascitic tumor cell suspension (1.03 × 10 6 viable cells) in the upper head region for solid form implantation. After 48 hours, mice received one of four treatments: (a) filtered water and no tumor implantation (negative control); (b) tumor inoculation and no treatment (tumor control); (c) intratumoral injection of NPCit (40 µL) containing 18 × 10 18 particles/mL and 13 minutes' exposure to CMagMHG, operating at 1MHz, 40 Oe field amplitude; (d) intratumoral injection of NPCit and 13 minutes' exposure to Magnetherm, assembled with 17-turn coil, capacitive box B22, operating under 330 kHz, 4.9 A, 25 V and maximum field strength 170 Oe. Treatments occurred once daily for three consecutive days. Tumor histopathology and semi-quantitative analysis of necrosis area served to assess tumor aggressiveness and regression. Possible acute side effects were assessed by animals' body and spleen weight and hemogram.Results: NPcit showed adequate biocompatibility and was effective in mediating RFHT and MHT, which promoted significant increases in necrosis area using both equipment types. Conclusion:Our findings evidence the potential use of NPcit mediating hyperthermia for future use as an adjuvant in breast cancer therapy.

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Section: Nanoparticles (Nps) Composed Of Ferromagnetic Materials and supporting

confidence: 84%

Section: Nanoparticles (Nps) Composed Of Ferromagnetic Materials and mentioning

confidence: 82%

Section: Hyperthermia Equipmentmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Efficacy of a Biocompatible Citrate-Functionalized Magnetic Fluid Mediating Radiofrequency Hyperthermia and Magnetohyperthermia to Treat Ectopic Ehrlich-Solid-Tumor-Bearing Elderly Mice

Neves¹,

Sousa²,

Vilela³

et al. 2017

J Cancer Sci Ther

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“…14, [24][25][26] Twenty hours after magnetic field exposure, cell viability was evaluated by the MTT assay. Untreated cells exposed or not to the AMF were the negative controls, and absorbance measurements were used to calculate the viable cells percentage after the treatment.…”

Section: Dna Fragmentation Analysismentioning

confidence: 99%

Co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy

Estevanato

Silva²,

Falqueiro³

et al. 2012

IJN

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Antitumor activities have been described in selol, a hydrophobic mixture of molecules containing selenium in their structure, and also in maghemite magnetic nanoparticles (MNPs). Both selol and MNPs were co-encapsulated within poly(lactic-co-glycolic acid) (PLGA) nanocapsules for therapeutic purposes. The PLGA-nanocapsules loaded with MNPs and selol were labeled MSE-NC and characterized by transmission and scanning electron microscopy, electrophoretic mobility, photon correlation spectroscopy, presenting a monodisperse profile, and positive charge. The antitumor effect of MSE-NC was evaluated using normal (MCF-10A) and neoplastic (4T1 and MCF-7) breast cell lines. Nanocapsules containing only MNPs or selol were used as control. MTT assay showed that the cytotoxicity induced by MSE-NC was dose and time dependent. Normal cells were less affected than tumor cells. Cell death occurred mainly by apoptosis. Further exposure of MSE-NC treated neoplastic breast cells to an alternating magnetic field increased the antitumor effect of MSE-NC. It was concluded that selol-loaded magnetic PLGA-nanocapsules (MSE-NC) represent an effective magnetic material platform to promote magnetohyperthermia and thus a potential system for antitumor therapy.

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“…Besides Mössbauer spectroscopy [1][2][3][4][5][6][7][8][9][10] other techniques used to characterize the above-mentioned magnetic DDS include those related to the morphological/crystalline aspects [11][12][13][14][15][16][17], the magnetic and magnetooptical response to DC/AC magnetic fields [18][19][20][21][22][23][24][25], and visible/microwave excitation [26][27][28][29][30][31][32]. Applications of the l-DDS discussed in this paper include cell-labeling [33][34][35], photodynamic therapy (PDT) [36][37][38][39][40], and magnetohyperthermia (MHT) of cancer cells and tissues [41][42][43][44].…”

Section: Introductionmentioning

confidence: 99%

Using Mössbauer spectroscopy as key technique in the investigation of nanosized magnetic particles for drug delivery

Morais

2008

Hyperfine Interact

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This paper describes how cobalt ferrite nanoparticles, suspended as ionic or biocompatible magnetic fluids, can be used as a platform to built complex nanosized magnetic materials, more specifically magnetic drug delivery systems. In particular, the paper is addressed to the discussion of the use of the Mössbauer spectroscopy as an extremely useful technique in supporting the investigation of key aspects related to the properties of the hosted magnetic nanosized particle. Example of the use of the Mössbauer spectroscopy in accessing information regarding the nanoparticle modification due to the empirical process which provides long term chemical stability is included in the paper.

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Proposal of a magnetohyperthermia system: preliminary biological tests

Cited by 24 publications

References 6 publications

Comparative Efficacy of a Biocompatible Citrate-Functionalized Magnetic Fluid Mediating Radiofrequency Hyperthermia and Magnetohyperthermia to Treat Ectopic Ehrlich-Solid-Tumor-Bearing Elderly Mice

Comparative Efficacy of a Biocompatible Citrate-Functionalized Magnetic Fluid Mediating Radiofrequency Hyperthermia and Magnetohyperthermia to Treat Ectopic Ehrlich-Solid-Tumor-Bearing Elderly Mice

Co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy

Using Mössbauer spectroscopy as key technique in the investigation of nanosized magnetic particles for drug delivery

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