Thunderstorms can generate intense electrical activity, hail, and result in substantial economic and human losses. The development of very short-term forecasting tools (nowcasting) is essential to provide information to alert systems in order to mobilize most efficiently the population. However, the development of nowcasting tools depends on a better understanding of the physics and microphysics of clouds and lightning formation and evolution. In this context, the objectives of this study are: (a) to describe the environmental conditions that led to a genesis of a thunderstorm that produce hail on 7 January 2012, in the Metropolitan Area of São Paulo (MASP) during the CHUVA-Vale campaign, and (b) to evaluate the thunderstorm microphysical properties and vertical structure of electrical charge. Data from different sources were used: field campaign data, such as S-band radar, and 2- and 3-dimensional lightning networks, satellite data from the Geostationary Operational Environmental Satellite-13 (GOES-13), the Meteosat Second Generation (MSG), and reanalysis of the European Centre for Medium-Range Weather Forecasts Reanalysis v5 (ERA5). The thunderstorm developed in a region of low-pressure due to the presence of a near-surface inverted trough and moisture convergence, which favored convection. Convective Available Potential Energy (CAPE) of 1053.6 J kg−1 at the start of the thunderstorm indicated that strong convective energy was present. Microphysical variables such as Vertically Integrated Liquid water content (VIL) and Vertically Integrated Ice (VII) showed peaks of 140 and 130 kg m−2, respectively, before the hail reached the surface, followed by a decrease, indicating content removal from within the clouds to the ground surface. The thunderstorm charge structure evolved from a dipolar structure (with a negative center between 4 and 6 km and a positive center between 8 and 10 km) to a tripolar structure (negative center between 6 and 7.5 km) in the most intense phase. The first lightning peak (100 flashes in 5 min−1) before the hail showed that there had been a lightning jump. The maximum lightning occurred around 18:17 UTC, with approximately 350 flashes 5 min−1 with values higher than 4000 sources 500 m−1 in 5 min−1. Likewise, the vertical cross-sections indicated that the lightning occurred ahead of the thunderstorm’s displacement (maximum reflectivity), which could be useful in predicting these events.