Propugnating Effect of Bark of <i>Rhizophora mucronata</i> Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG<sub>2</sub> Cell Lines

Jairaman, Chitra; Yacoob, Syed Ali Mohamed; Venkatraman, Anuradha; Nagarajan, Yogananth; Gnanadesigan, Murugesan

doi:10.3831/kpi.2019.22.005

Cited by 1 publication

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“…Based on a previous report, using human hepatocarcinoma cell line HepG2, the plant parts of Rhizophora mucronata Lam. (R. mucronata) (also known as Mangrove), including leaves, roots, flowers, bark and fruits, were shown to have promising therapeutic values for elephantiasis, hepatitis, ulcers through its antibacterial and anti-inflammatory activities, and the ability to neutralize toxicants, including ethanol intoxication [22,23]. However, no studies have explored the in vivo biological protective impact of the bark extract of Rhizophora mucronata (BERM) against ethanol-induced liver injury and hepatotoxicity.…”

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confidence: 99%

Rhizophora mucronata Lam. (Mangrove) Bark Extract Reduces Ethanol-Induced Liver Cell Death and Oxidative Stress in Swiss Albino Mice: In Vivo and In Silico Studies

Jairaman¹,

Alehaideb

Yacoob³

et al. 2022

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The bark extract of Rhizophora mucronata (BERM) was recently reported for its prominent in vitro protective effects against liver cell line toxicity caused by various toxicants, including ethanol. Here, we aimed to verify the in vivo hepatoprotective effects of BERM against ethanol intoxication with the prediction of potential targets employing in silico studies. An oral administration of different concentrations (100, 200 and 400 mg/kg body weight) of BERM before high-dose ethanol via intraperitoneal injection was performed in mice. On day 7, liver sections were dissected for histopathological examination. The ethanol intoxication caused liver injury and large areas of necrosis. The pre-BERM administration decreased the ethanol-induced liver damage marker tumor necrosis factor-alpha (TNF-α) expression, reduced hepatotoxicity revealed by nuclear deoxyribonucleic acid (DNA) fragmentation and decreased oxidative stress indicated by malondialdehyde and glutathione contents. Our in silico studies have identified BERM-derived metabolites exhibiting the highest predicted antioxidant and free radical scavenger activities. Molecular docking studies showed that most of the metabolites were predicted to be enzyme inhibitors such as carbonic anhydrase inhibitors, which were reported to stimulate the antioxidant defense system. The metabolites predominantly presented acceptable pharmacokinetics and safety profiles, suggesting them as promising new antioxidant agents. Altogether, the BERM extract exerts antioxidative activities and shows promising hepatoprotective effects against ethanol intoxication. Identification of related bioactive compounds will be of interest for future use at physiological concentrations in ethanol-intoxicated individuals.

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Section: Introductionmentioning

confidence: 99%

Rhizophora mucronata Lam. (Mangrove) Bark Extract Reduces Ethanol-Induced Liver Cell Death and Oxidative Stress in Swiss Albino Mice: In Vivo and In Silico Studies

Jairaman¹,

Alehaideb

Yacoob³

et al. 2022

Metabolites

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Propugnating Effect of Bark of Rhizophora mucronata Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG₂ Cell Lines

Cited by 1 publication

References 12 publications

Rhizophora mucronata Lam. (Mangrove) Bark Extract Reduces Ethanol-Induced Liver Cell Death and Oxidative Stress in Swiss Albino Mice: In Vivo and In Silico Studies

Rhizophora mucronata Lam. (Mangrove) Bark Extract Reduces Ethanol-Induced Liver Cell Death and Oxidative Stress in Swiss Albino Mice: In Vivo and In Silico Studies

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Propugnating Effect of Bark of Rhizophora mucronata Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG2 Cell Lines

Cited by 1 publication

References 12 publications

Rhizophora mucronata Lam. (Mangrove) Bark Extract Reduces Ethanol-Induced Liver Cell Death and Oxidative Stress in Swiss Albino Mice: In Vivo and In Silico Studies

Rhizophora mucronata Lam. (Mangrove) Bark Extract Reduces Ethanol-Induced Liver Cell Death and Oxidative Stress in Swiss Albino Mice: In Vivo and In Silico Studies

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Propugnating Effect of Bark of Rhizophora mucronata Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG₂ Cell Lines