Proses Deparafinasi Sediaan Jaringan Ginjal Dengan Dan Tanpa Pemanasan Menggunakan Mineral Oil  Pada Pewarnaan Hematoksilin-Eosin

Ariyadi, Tulus

doi:10.54350/jkr.v11i2.104

Cited by 5 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hal tersebut mengindikasikan minyak kenanga dapat digunakan sebagai alternatif xylol atau xylene pada proses deparafinisasi dengan pengecatan hematoksilin-eosin. Hasil ini sejalan dengan beberapa penelitian sebelumnya, salah satunya oleh Damayanti et al (2021) yang melaporkan penggunaan mineral oil dapat dijadikan sebagai pengganti xylol dengan pemanasan 50°C yang menghasilkan 100% preparat dengan kualitas baik. Selanjutnya penelitian oleh Nuroini et al (2019) yang menggunakan minyak zaitun dengan pemanasan 60°C sebagai alternatif pengganti xylol, minyak zaitun menunjukkan kualitas preparat yang sama pada bagian inti, sitoplasma, dan glomerulus.…”

Section: Keterangan Inti Sel Sitoplasmaunclassified

“…Hasil ini sejalan dengan beberapa penelitian sebelumnya, salah satunya oleh Damayanti et al (2021) yang melaporkan penggunaan mineral oil dapat dijadikan sebagai pengganti xylol dengan pemanasan 50°C yang menghasilkan 100% preparat dengan kualitas baik. Selanjutnya penelitian oleh Nuroini et al (2019) yang menggunakan minyak zaitun dengan pemanasan 60°C sebagai alternatif pengganti xylol, minyak zaitun menunjukkan kualitas preparat yang sama pada bagian inti, sitoplasma, dan glomerulus. Minyak zaitun mengandung asam oleat yang bersifat sama dengan eosin sehingga sitoplasma mampu terwarnai dengan baik.…”

Section: Keterangan Inti Sel Sitoplasmaunclassified

See 1 more Smart Citation

The Effectiveness of Ylang Ylang Oil (Cananga Odorata) as a Deparaffinizing Agent in Hematoxylin-Eosin Staining

Putri,

Diyanah,

Iswara

2024

JLM

View full text Add to dashboard Cite

Histological staining usually requires deparaffinization before starting the staining process. Deparaffinization is a paraffin removal step that often uses a xylol solution. Deparaffinization with xylol has disadvantages, including being toxic, harmful to the human body, and hazardous to the environment. A safer alternative to xylol is required. Ylang-ylang oil, which contains β-caryophyllene, is non-polar and can be used as a substitute for xylol. This study aimed to compare the staining quality of guinea pig liver tissue slides with and without heating as a deparaffinization agent in hematoxylin-eosin (HE) staining. The research set up a quasi-experimental approach using samples of guinea pig liver tissue slides. The sample size was calculated using the Federer formula (n-1) (t-1) ≥ 15, resulting in a total sample of 27 slides divided into three treatment groups: xylol, ylang-ylang oil with and without heating. The Kruskal-Wallis test was used to examine the data. The quality staining of guinea pig liver slides deparaffinized with xylol was 100% good, and deparaffinization of ylang-ylang oil with heating was 100% good, whereas deparaffinization with ylang-ylang oil without heating was 11.1% less good, and the quality was good with an 88.9%. The normality test indicated that the data were not normally distributed (p0.05). The Kruskal-Wallis test was then used.

show abstract

Section: Keterangan Inti Sel Sitoplasmaunclassified

The Effectiveness of Ylang Ylang Oil (Cananga Odorata) as a Deparaffinizing Agent in Hematoxylin-Eosin Staining

Putri,

Diyanah,

Iswara

2024

JLM

View full text Add to dashboard Cite

show abstract

“…Furthermore, the slides were put into 80%, 90%, and 100% alcohol. Last, slides were given entellan to glue the slide material with the glass object (Ariyadi & Suryono, 2017).…”

Section: Preparation Of Cardiac Histology Slide With Hematoxylin Eosi...mentioning

confidence: 99%

Effect Soursop (Annona muricata L.) Leaf Aqueous Extract (SLAE) on Remodeling of Ventricle Heart Tissue in Obesity Rats Model

Haq

Rosidah²,

Damayanti

2022

nuscientech

View full text Add to dashboard Cite

A high-fat and high-fructose diet can lead to obesity which contributes to an increased risk of heart failure complications. Soursop leaves are known to have the potential of antioxidant, but research about the effects of soursop leaf on complications of heart failure has never been studied. This study evaluated whether soursop leaf aqueous extract can inhibit the total of cardiomyocyte necrosis, cardiomyocyte diameter, and density of cardiac collagen in obese rats. The heart organs of obese rats that have been paraffin, which randomly divided into normal group, obesity group, and soursop leaf aqueous extract (SLAE) group with dose I (100 mg/kgbw), II (200 mg/kgbw), and III (400 mg/kgbw) (n=6 rats). The rats were induced by high-fat high-fructose and SLI diets for 10 weeks. The number of necrosis and diameter cardiomyocytes were measured by using Hematoxylin Eosin staining, while the density of cardiac collagen was measured by Masson's Trichrome staining and then observed with a trinocular and dot side microscope at 200x and 400x magnification. Statistical analysis using One Way ANOVA and continued by LSD test. We found that group SLAE with doses I, II, and III significantly reduced the number of cardiomyocyte necrosis and cardiac collagen density compared to the obesity group. The SLI group with doses II and III significantly reduced the diameter of cardiomyocytes compared to the obesity group, while the SLAE group with the dose I was unable to reduce the diameter of cardiomyocytes. In conclusion, Soursop leaf aqueous extract can inhibit the increase of cardiac collagen density, number necrosis and diameter cardiomyocyte.

show abstract

“…6 elevated glucose levels can cause other metabolic disorders and produce free radicals. 7 Hyperglycemic conditions can lead to oxidative stress because excess reactive oxygen species (ROS) interfere with the work of antioxidants, and high glucose conditions can worsen the damaging effects on cells and organs.…”

Section: Introductionmentioning

confidence: 99%

Potential Bark Cinnamomum burmanii in Regenerating Damaged Liver Cells of Mice (Mus musculus) Diabetes Mellitus Model

Ukratalo

Kaihena

Sirait

et al. 2023

Pharmaceutical and Clinical Journal of Nusantara

View full text Add to dashboard Cite

Diabetes mellitus is a chronic disease that can quickly increase the prevalence of complications in sufferers, such as liver damage. This study aims to examine the effect of methanol extract cinnamon bark (Cinnamomum burmanii Bl) in regenerating liver cell damage mice (Mus musculus) Diabetes Mellitus through the histological picture. 20 mice were divided into 5 groups which consisted of a negative control group, Positive control and a group of mice that were given methanol extract bark Cinnamomum burmannii dose of 125 mg/head/day, 250 mg/head/Day and 500 mg/head/day. In the positive control group and the group of mice that will be given extract streptozotocin injected dose of 0.2 ml and observed blood sugar levels if sugar levels have increased then given methanol extract bark Cinnamomum burmannii with a dose that has been determined and blood sugar levels were measured. On the last day, surgery is performed to remove the liver, after which histological preparations are made. The results showed that the administration of bark extract Cinnamomum burmanii can regenerate damaged liver cells of mice due to diabetes mellitus. This is due to the content of secondary metabolite compounds contained in the bark of Cinnamomum burmannii.

show abstract

Proses Deparafinasi Sediaan Jaringan Ginjal Dengan Dan Tanpa Pemanasan Menggunakan Mineral Oil Pada Pewarnaan Hematoksilin-Eosin

Cited by 5 publications

References 0 publications

The Effectiveness of Ylang Ylang Oil (Cananga Odorata) as a Deparaffinizing Agent in Hematoxylin-Eosin Staining

The Effectiveness of Ylang Ylang Oil (Cananga Odorata) as a Deparaffinizing Agent in Hematoxylin-Eosin Staining

Effect Soursop (Annona muricata L.) Leaf Aqueous Extract (SLAE) on Remodeling of Ventricle Heart Tissue in Obesity Rats Model

Potential Bark Cinnamomum burmanii in Regenerating Damaged Liver Cells of Mice (Mus musculus) Diabetes Mellitus Model

Contact Info

Product

Resources

About