Proses Fiksasi pada Pemeriksaan Histopatologik

Musyarifah, Zulda; Agus, Salmiah

doi:10.25077/jka.v7i3.900

Cited by 3 publications

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“…Larutan fiksatif masih terlihat jernih dan volumenya tidak banyak berkurang. Larutan NBF 10% merupakan larutan fiksatif terbaik untuk pemeriksaan histopatologi karena bersifat stabil, mempunyai komposisi ion hidrogen (pH) netral, serta memiliki kemampuan penetrasi 0,5-1 mm/ jam (Musyarifah & Agus, 2018). Hasil penilaian preparat pada penggunaan ulang NBF 10% 2 kali mengalami penurunan kualitas.…”

Section: Pembahasanunclassified

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The effect of reusing formaldehyde fixative solution on the quality of histopathological slides and the amount of waste produced

Hardi,

Wiryanti,

Durachim

et al. 2024

curr biomed

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Background: Neutral buffered formalin (NBF) 10% fixative solution is widely used in histopathological slides. The fixation process generates liquid waste of NBF 10% and solid waste of tissue remnants. Objective: The research aimed to assess the reuse of NBF 10% fixative solution on the quality of histopathological slides and calculate the amount of waste produced. Methods: Treatments included single-use of fixative solution (control), reuse for 1, 2, and 3 times. Ten sample slides were prepared for each treatment, consisting of intestinal tissue, uterine fibroids, prostate, uterus, ovarian cyst, portio vaginalis cervicis, thyroid, rectum, breast fibroadenoma, and gallbladder tissues. Tissues were fixed with NBF 10% and processed histologically with hematoxylin-eosin staining. Liquid waste of NBF 10% and solid waste of tissue remnants were quantified. Histopathological slide quality was measured under a microscope for nuclear and cytoplasmic clarity, staining intensity, and color uniformity. Results: Control slides exhibited good quality with clearly blue-stained nuclei, pink cytoplasm, no color accumulation, and uniform staining across fields of view. Reused NBF 10% slides experienced a decrease in quality compared to the control but were still usable for diagnosis. Slides reused 2 and 3 times showed poor quality, making diagnosis difficult. Fixation resulted in 299.0 liters of liquid waste of NBF 10% and 64.9 kilograms of solid tissue remnants. Conclusion: Reusing NBF 10% decreases histological slide quality, though reuse once still allows for diagnosis. Reusing 10% NBF for tissue fixation can reduce the liquid waste of fixative solution and solid tissue waste.

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Section: Pembahasanunclassified

“…Jika terjadi kesalahan maka tidak dapat diperbaiki pada tahapan selanjutnya. Pada saat ini, larutan neutral buffered formalin (NBF) 10% merupakan larutan fiksatif yang terbaik untuk pemeriksaan histopatologi (Khristian & Inderiati, 2017;Musyarifah & Agus, 2018).…”

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The effect of reusing formaldehyde fixative solution on the quality of histopathological slides and the amount of waste produced

Hardi,

Wiryanti,

Durachim

et al. 2024

curr biomed

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“…Fiksasi merupakan langkah dasar pada studi patologi dan sangat berguna dalam mencegah autolysis, degradasi jaringan maupun komponen jaringan sehingga dapat diamati secara anatomis maupun histologi (Musyarifah & Agus, 2018). Fiksasi yang baik ialah yang mampu mempertahankan sel serta komponen jaringan pada kondisi "life-like state" yang berarti kondisi sediaan yang diperiksa akan memberikan hasil yang sama seperti kondisi pada saat jaringan masih hidup (Musyarifah & Agus, 2018).…”

Section: Pendahuluanunclassified

“…Kelebihan larutan Carnoy adalah memiliki daya penetrasi yang cepat membuat proses fiksasi lebih cepat selesai, menurut Musyarifah dan Agus (2018) larutan Carnoy memiliki waktu fiksasi sekitar 1-4 jam . Kelebihan yang lain dapat melisiskan eritrosit serta melarutkan lipid, mempunyai kemampuan mempertahankan inti sel dan menahan glikogen (Musyarifah & Agus, 2018). Fiksatif Carnoy cepat beraksi dan dapat digunakan untuk spesimen mendesak untuk pemrosesan parafin dalam lima jam (Ahmed & Mohammed, 2011).…”

Section: Pendahuluanunclassified

“…Pada penelitian kali ini mengambil jaringan hepar pada hewan coba mencit (Mus musculus) karena menurut Fajrina, Ariyadi, & Nuroini (2018) hepar mempunyai struktur yang lunak, serta menurut Jusuf (2009) terdapat komponen yang dapat mengganggu proses fiksasi antara lain darah, lemak, serta kandungan air yang tinggi. Tidak hanya itu, banyak faktor yang mempengaruhi proses fiksasi seperti konsentrasi ion hidrogen atau pH netral, suhu penetrasi fiksasi, penetration rate dan ketebalan pemotongan sekitar 3-4 mm, konsentrasi larutan, volume fiksasi dengan perbandingan 20:1, dan waktu fiksas (Musyarifah & Agus, 2018).…”

Section: Pendahuluanunclassified

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Histology of Mice (Mus Musculus) Liver Tissue Fixed with Carnoy's Solution With Variation of 4 Hours, 8 Hours and 12 Hours

Afrida

Priyatno

2021

JLM

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Histopathological examination is a routine examination process for each tissue in the anatomic pathology laboratory. The stages in making histology preparations are fixation stages. The type of solution that can be used as a fixative solution other than 10% NBF is Carnoy's solution. Carnoy's solution is a fixative solution with a relatively fast fixation process of about 1-4 hours. The advantages of Carnoy's solution can lyse erythrocytes and dissolve lipids, have the ability to maintain cell nuclei, and retain glycogen. The purpose of this study was to determine the histology of the liver tissue preparations of mice (Mus Musculus) which were fixed using Carnoy's solution with time variations of 4 hours, 8 hours, and 12 hours. This is an observational research with descriptive research design criteria. The results of the histology of the liver tissue preparations of mice (Mus Musculus) which were fixed using the 10% NBF solution group obtained 100% good preparations. In the Carnoy solution group with a time of 4 hours, it gave 2.2% results of poor preparations and 97.78% of good preparations. While the treatment group with Carnoy's solution with a time of 8 hours obtained as many as 11.11% of preparations that were not good and 88.89% of good preparations. and in the Carnoy solution group with a time of 12 hours, there were 44.44% of the poor preparations and 55.56% of the good preparations.

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Histopathological Profile of Therapeutic Doses of Methanolic Extract Combination of Tea and Mango Mistletoes in Cardiac of Preventive Hypertensive Rats (DOCA-salt) Model

Hasanah

Sjakoer²,

Mubarakati³

2022

IJMLST

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Tea and mango mistletoes have to lower blood pressure by improving oxidative stress and endothelial dysfunction. The flavonoid compounds found in these two mistletoes act as antihypertensives. Hypertension is a risk factors that influences the incidence of cardiac disease. This study examines the histopathological of the cardiac of hypertensive rats (preventive model) after treatment of methanolic extract combination of tea and mango mistletoes. This study used a completely randomized experimental design with 25 Wistar rats in total for five treatments, namely negative control (-), positive control (+), treatment 1 (dose of 50 mg/kg BW), treatment 2 (doses of 100 mg/kg BW), and treatment 3 (doses of 200 mg/kg BW). In this study, Deoxycorticosterone acetate (DOCA) were used to induce hyperttension in experimental animals. Data were analysed with one-way ANOVA test using JAMOVI (version 1.1.9.0) and posteriori. There was a significant difference between all groups (p<0.05). Administration of a methanolic extract combination of tea and mango mistletoes as the preventive model can significantly reduce hypertrophy of cardiac muscle cells. This effect occured with a dose of 50 mg/kg BW, the optimal dose to reduce hypertrophy of cardiac muscle cells in Wistar rats male.

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Proses Fiksasi pada Pemeriksaan Histopatologik

Cited by 3 publications

References 0 publications

The effect of reusing formaldehyde fixative solution on the quality of histopathological slides and the amount of waste produced

The effect of reusing formaldehyde fixative solution on the quality of histopathological slides and the amount of waste produced

Histology of Mice (Mus Musculus) Liver Tissue Fixed with Carnoy's Solution With Variation of 4 Hours, 8 Hours and 12 Hours

Histopathological Profile of Therapeutic Doses of Methanolic Extract Combination of Tea and Mango Mistletoes in Cardiac of Preventive Hypertensive Rats (DOCA-salt) Model

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