PROSPECÇÃO DE TÉCNICAS MOLECULARES (qPCR e FISH) A SEREM UTILIZADAS EM AMOSTRAS AMBIENTAIS PARA PESQUISA NA ÁREA DE BIOTECNOLOGIA

Lima, Luciana Reis; Almeida, Paulo Fernando de; Matos, Josilene Borges Torres Lima

doi:10.9771/s.cprosp.2016.009.010

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, Sybr Green ® binds to all double-stranded DNA (including dimers) and water samples contain organic and inhibitors substances that can be co-concentrated during sample processing, affecting qPCR, thus causing nonspecific reactions, and this has been identified as one of the problems in the use of Sybr Green ® [34]. TaqMan ® has the advantage of being more sensitive when compared to Sybr Green ® in virus detection [39][40][41]. For the detection of environmental viruses in different matrices, the TaqMan ® probe is widely preferred [39][40][41].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence of Enterovirus in Water Consumed in Rural Areas in a State in the Midwest Region of Brazil

et al. 2023

View full text Add to dashboard Cite

Individuals in rural communities often obtain water from surface and groundwater sources, where the microbial quality is often unknown. Enteric viruses are among the main pathogenic microorganisms responsible for waterborne disease outbreaks. Thus, the objective of this work was to search for enterovirus in water samples from 25 rural communities. For this, 160 water samples were collected. Detection and quantification of the enterovirus (EV) were performed through molecular tests using the two main amplification reagents for qPCR. The prevalence of EV was identified in 4.4% (7/160) of the samples when Sybr Green® was used, all in groundwater sources. Additionally, EV was found in 9.7% of shallow tubular wells, 3.8% of deep tubular wells, 4.3% of shallow dug wells and 5.9% of spring water. When using TaqMan®, there was no amplification of the EV cDNA. Conclusions: Sybr Green®, being a more accessible reagent, has a greater predilection for molecular tests, but the study showed that Taqman® could suffer less interference from environmental samples, resulting in more reliable values of viral quantification. In this context, the detection of EV in groundwater can help in monitoring the virus in this source, in addition to helping managers of these communities in decision making.

show abstract

Section: Introductionmentioning

confidence: 99%

“…TaqMan ® has the advantage of being more sensitive when compared to Sybr Green ® in virus detection [39][40][41]. For the detection of environmental viruses in different matrices, the TaqMan ® probe is widely preferred [39][40][41].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Enterovirus in Water Consumed in Rural Areas in a State in the Midwest Region of Brazil

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Diversas técnicas já foram consideradas para quantificar espécies BRS em amostras coletadas da indústria petrolífera, tais como ensaios baseados em reações imunoenzimáticas; pelo consumo de ATP; por marcação com radioisótopos, e com uso de sequenciamento para identificação de espécies (LIMA, 2016;KOTU;MANNAN;JAYARAMAN, 2019).…”

Section: Cultivo E Detecção De Brs Em Microescalaunclassified

Desenvolvimento e implantação de metodologia para avaliação da atividade antimicrobiana de substâncias frente a microrganismos envolvidos em processos de biocorrosão

Simon

View full text Add to dashboard Cite

show abstract

PROSPECÇÃO DE TÉCNICAS MOLECULARES (qPCR e FISH) A SEREM UTILIZADAS EM AMOSTRAS AMBIENTAIS PARA PESQUISA NA ÁREA DE BIOTECNOLOGIA

Cited by 2 publications

References 0 publications

Prevalence of Enterovirus in Water Consumed in Rural Areas in a State in the Midwest Region of Brazil

Prevalence of Enterovirus in Water Consumed in Rural Areas in a State in the Midwest Region of Brazil

Desenvolvimento e implantação de metodologia para avaliação da atividade antimicrobiana de substâncias frente a microrganismos envolvidos em processos de biocorrosão

Contact Info

Product

Resources

About