Prospect of Stem Cell Conditioned Medium in Regenerative Medicine

Pawitan, Jeanne Adiwinata

doi:10.1155/2014/965849

Cited by 362 publications

(370 citation statements)

References 49 publications

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“…Produk atau faktor tersekresi tersebut dapat digunakan untuk memperbaiki jaringan atau organ yang mengalami kerusakan. Menurut Pawitan (2014), ekstrak media penumbuh sel punca mesenkimal merupakan serangkaian faktor atau molekul yang disekresikan oleh sel punca mesenkimal yang terkandung dalam media yang dikondisikan, tanpa sel punca itu sendiri. Berdasarkan kajian di atas, maka penelitian ini bertujuan untuk mengetahui efektivitas ekstrak media penumbuh sel punca mesenkimal terhadap aktivitas radikal bebas dari MDA di dalam plasma dan jaringan ginjal tikus yang diinduksi agen diabetogenik yaitu streptozotosin-nikotinamid (STZ-NA).…”

Section: Pendahuluanunclassified

“…Merurut Pawitan (2014), EMPSPM mengandung berbagai macam molekul tersekretori diantaranya Gambar 5 Profil Kadar Malondialdehid pada Ginjal Tikus Diabetes Mellitus Tipe 2 pada hari ke 43. Grafik menunjukkan bahwa kadar MDA dalam organ ginjal pada hari ke 43 pascaterapi EMPSPM dengan dosis 0,05; 0,1; dan 0,2 memiliki nilai yang lebih kecil dan berbeda signifikan dengan kontrol DMT2 (P<0,05).…”

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Kadar Malondialdehid Tikus Diabetes Melitus Tipe 2 dengan Terapi Ekstrak Media Penumbuh Sel Punca Mesenkimal

Fitriana¹,

Wijayanti²,

Sari³

et al. 2017

Acta Vet Indones

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ABSTRAKBerbagai penelitian menunjukkan bahwa ekstrak media penumbuh sel punca mesenkimal (EMP-SPM), tanpa sel punca itu sendiri, telah ditemukan terdapat berbagai faktor tropik hasil sekresi sel punca mesenkimal di dalam media kultur yang dapat meregenerasi jaringan yang rusak. Penelitian ini bertujuan untuk mengetahui gambaran kadar malondialdehid (MDA) dalam plasma dan ginjal tikus diabetes melitus tipe 2 (DMT2) dengan terapi EMPSPM. Dua puluh lima ekor tikus wistar digunakan dalam penelitian ini dibagi menjadi 5 kelompok yaitu kelompok DMT2 + 0,05 ml/kg BB EMPSPM (0,05); kelompok DMT2 + 0,1 ml/kg BB EMPSPM (0,1); kelompok DMT2 + 0,2 ml/kg BB EMPSPM (0,2), kelompok kontrol DMT2 (DMT2), dan kelompok sehat (KS). Induksi DMT2 dengan menggunakan streptozotosinnikotinamid (STZ-NA). Terapi mulai dilakukan pada hari ke 7 setelah kondisi DM tercapai, diberikan 4 kali dengan selang waktu 7 hari secara intraperitoneal. Data kadar glukosa darah dan MDA dianalisi secara statistik. Hasil penelitian menunjukkan bahwa induksi DMT2 dengan STZ-NA dapat menaikkan kadar glukosa dan MDA dalam darah (P<0,05). Terapi 0,05; 0,1; dan 0,2 ml/kg BB EMPSPM menunjukkan dapat menurunkan kadar glukosa darah, kadar MDA plasma dan ginjal (P<0,05). Kadar 0,2 ml/kg BB EMPSPM menunjukkan aktifitas lebih baik dalam menurunkan kadar glukosa darah dan MDA. Berdasarkan penelitian ini, EMPSPM dapat menurunkan kadar glukosa darah serta kadar MDA dalam darah dan ginjal tikus DMT2.Kata kunci: EMPSPM, streptozotosin, nikotinamid, diabetes mellitus tipe 2, malondialdehid ABSTRACTVarious studies show that Mesenchymal Stem Cell-Conditioned Media (MSC-CM)), without the stem cells themselves, has been found to contain various trophic factor secretion results in a mesenchymal stem cell culture medium that can regenerate damaged tissue. This study aims to describe the levels of malondialdehyde (MDA) in plasma and kidneys of type 2 diabetes mellitus rats (T2DM) with MSC-CM therapy. Twenty five male Wistar rats used in this study were divided into 5 groups: T2DM + 0.05 ml/kg BW MSC-CM (0.05); T2DM + 0.1 ml/kg BW MSC-CM (0.1); T2DM + 0.2 ml/kg BB MSC-CM (0.2); the control group with T2DM (DMT2), and healthy group (KS). Induction of T2DM by using streptozotocin-nicotinamide (STZ-NA). Treatment started on day 7 after the DM condition is reached, given 4 times with an interval of 7 days, intraperitoneally. The datas blood glucose and MDA levels will be analyzed statistically. The results showed that the induction of T2DM with STZ-NA can increase glucose and MDA levels in the blood (P <0.05). Therapy 0.05; 0.1; and 0.2 ml/kg BW MSC-CM shown to reduce blood glucose levels, plasma MDA levels and kidney (P <0.05). Levels of 0.2 ml/kg MSC-CM showed better activity in lowering blood glucose and MDA levels. Based on this research, MSC-CM can lower blood glucose levels and MDA levels in the blood and kidneys of T2DM rats.

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Section: Pendahuluanunclassified

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Kadar Malondialdehid Tikus Diabetes Melitus Tipe 2 dengan Terapi Ekstrak Media Penumbuh Sel Punca Mesenkimal

Fitriana¹,

Wijayanti²,

Sari³

et al. 2017

Acta Vet Indones

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“…The use of secretome containing CM has several advantages when compared to the use of stem cells. Briefly, CM can be manufactured, freezedried, packaged, and transported with greater ease [1]. Moreover, as it is devoid of cells there is a lower risk of host rejection.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemistry Evaluation of TGF-β1, SOX-9, Type II Collagen and Aggrecan in Cartilage Lesions Treated with Conditioned Medium of Umbilical Cord Mesencyhmal Stem Cells in Wistar Mice (Rattus novergicus)

Soetjahjo

Hidayat

Sujuti

et al. 2018

J.Trop.Life.Science

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Currently, umbilical cord mesenchymal stem cells have the potential to be used as treatment options for any cartilage lesion. This research aimed to evaluate the effects of conditioned medium from umbilical cord mesenchymal stem cells (UC-MSC) on damaged cartilage through the expression of proteins TGF-β1, SOX-9, type II collagen and aggrecan, which are known to be related to chondrogenesis. UC-MSC were isolated from 19-days-pregnant Wistar mice and were cultured using the standard procedure to obtain 80% confluence. Subsequently, the culture was confirmed through a microscopic examination that was driven to be an embryoid body to obtain a pre-condition medium. This research utilized 3-month-old male Wistar mice and was categorized into 6 groups (3 control and 3 treatment groups). Each animal had surgery performed to create a femur condyle cartilage defect. The treatment groups were administered a dose of stem cells at 1 mL/kg. Next, immunohistochemical (IHC) staining was performed to examine the expression of TGF-β1, SOX-9, type II collagen and aggrecan in the 2 nd , 3 rd , and 4 th month of evaluation. The results were analyzed statistically using ANOVA test. For each of the treatment groups, there was increased expression (p < 0.05) in all proteins TGF-β1, SOX-9, type II collagen and aggrecan when compared with control groups at the 2 nd , 3 rd , and 4 th month of evaluation. Pre-conditioned medium from UC-MSC potentially increases the expression of TGF-β1, SOX-9, type II collagen and aggrecan in the damaged cartilage of Wistar mice.

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“…Some studies investigated the effect of MSC conditioned medium (MSC-CM) on various diseases/conditions such as wound healing and cardiac and ischemic diseases (5). The therapeutic effects of MSC-CM are due to the panel of growth factors and cytokines presented in it.…”

mentioning

confidence: 99%

Prospect of Mesenchymal Stem Cells Conditioned Medium in Tissue Regeneration

Sanchooli

2017

Gene Cell Tissue

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Prospect of Stem Cell Conditioned Medium in Regenerative Medicine

Cited by 362 publications

References 49 publications

Kadar Malondialdehid Tikus Diabetes Melitus Tipe 2 dengan Terapi Ekstrak Media Penumbuh Sel Punca Mesenkimal

Kadar Malondialdehid Tikus Diabetes Melitus Tipe 2 dengan Terapi Ekstrak Media Penumbuh Sel Punca Mesenkimal

Immunohistochemistry Evaluation of TGF-β1, SOX-9, Type II Collagen and Aggrecan in Cartilage Lesions Treated with Conditioned Medium of Umbilical Cord Mesencyhmal Stem Cells in Wistar Mice (Rattus novergicus)

Prospect of Mesenchymal Stem Cells Conditioned Medium in Tissue Regeneration

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