Prospecting fungal parasites of the potato cyst nematode <i>Globodera pallida</i> using a rapid screening technique

Kooliyottil, Rinu; Dandurand, Louise-Marie; Knudsen, G. R.

doi:10.1002/jobm.201600683

Cited by 13 publications

(8 citation statements)

References 34 publications

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“…B. thuringiensis produces proteinaceous protoxin crystals (called crystal protein or Cry protein) that cause lysis of the intestine and the nematode's death (Griffitts et al, 2005;Vachon et al, 2012). P. cucumerina was also isolated from the egg masses of M. incognita (Yu and Coosemans, 1998) and eggs of G. pallida (Kooliyottil et al, 2017), and has been found to infect eggs of G. rostochiensis (Atkins et al, 2003). The microbial community analysis using bacterial 16S rRNA gene and ITS amplicon sequencing from G. pallida females showed a dominance of diverse microbiota such as Burkholderia, Bosea, Rhizobium, Devosia, Ralstonia, and Streptomyces, and the fungal genera Davidiella, Hirsutella, Malassezia, Microdochium, Monographella, and Penicillium in potato monoculture soil (Eberlein et al, 2016).…”

Section: Microbes Identified In a Direct Association To Ppnmentioning

confidence: 99%

Plants and Associated Soil Microbiota Cooperatively Suppress Plant-Parasitic Nematodes

2020

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Disease suppressive soils with specific suppression of soil-borne pathogens and parasites have been long studied and are most often of microbiological origin. As for the plant-parasitic nematodes (PPN), which represent a huge threat to agricultural crops and which successfully defy many conventional control methods, soil progression from conducive to suppressive state is accompanied by the enrichment of specific antagonistic microbial consortia. However, a few microbial groups have come to the fore in diminishing PPN in disease suppressive soils using culture-dependent methods. Studies with cultured strains resulted in understanding the mechanisms by which nematodes are antagonized by microorganisms. Recent culture-independent studies on the microbiome associated with soil, plant roots, and PPN contributed to a better understanding of the functional potential of disease suppressive microbial cohort. Plant root exudation is an important pathway determining host-microbe communication and plays a key role in selection and enrichment of a specific set of microbial antagonists in the rhizosphere as first line of defense against crop pathogens or parasites. Root exudates comprising primary metabolites such as amino acids, sugars, organic acids, and secondary metabolites can also cause modifications in the nematode surface and subsequently affect microbial attachment. A positive interaction between hosts and their beneficial root microbiota is correlated with a low nematode performance on the host. In this review, we first summarized the historical records of nematodesuppressive soils and then focused on more recent studies in this aspect, emphasizing the advances in studying nematode-microbe interactions over time. We highlighted nematode biocontrol mechanisms, especially parasitism, induced systemic resistance, and volatile organic compounds using microbial consortia, or bacterial strains of the genera Pasteuria, Bacillus, Pseudomonas, Rhizobium, Streptomyces, Arthrobacter, and Variovorax, or fungal isolates of Pochonia, Dactylella, Nematophthora, Purpureocillium, Trichoderma, Hirsutella, Arthrobotrys, and Mortierella. We discussed the importance of root exudates in plant communication with PPN and soil microorganisms, emphasizing their role in microbial attachment to the nematode surface and subsequent events of

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Section: Microbes Identified In a Direct Association To Ppnmentioning

confidence: 99%

Plants and Associated Soil Microbiota Cooperatively Suppress Plant-Parasitic Nematodes

2020

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“…Furthermore, P. cucumerina AR1 was described as a biological control agent against potato cyst nematodes (Atkins et al, 2003; Jacobs et al, 2003; Dandurand and Knudsen, 2016; Kooliyottil et al, 2017). It was used as a bioherbicide in agricultural crops and pastures (Bailey K. et al, 2017; Bailey K.L.…”

Section: Discussionmentioning

confidence: 99%

Nicosulfuron Degradation by an Ascomycete Fungus Isolated From Submerged Alnus Leaf Litter

et al. 2018

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Nicosulfuron is a selective herbicide belonging to the sulfonylurea family, commonly applied on maize crops. Its worldwide use results in widespread presence as a contaminant in surface streams and ground-waters. In this study, we isolated, for the first time, the Plectosphaerella cucumerina AR1 nicosulfuron-degrading fungal strain, a new record from Alnus leaf litter submerged in freshwater. The degradation of nicosulfuron by P. cucumerina AR1 was achieved by a co-metabolism process and followed a first-order model dissipation. Biodegradation kinetics analysis indicated that, in planktonic lifestyle, nicosulfuron degradation by this strain was glucose concentration dependent, with a maximum specific degradation rate of 1 g/L in glucose. When grown on natural substrata (leaf or wood) as the sole carbon sources, the Plectosphaerella cucumerina AR1 developed as a well-established biofilm in 10 days. After addition of nicosulfuron in the medium, the biofilms became thicker, with rising mycelium, after 10 days for leaves and 21 days for wood. Similar biofilm development was observed in the absence of herbicide. These fungal biofilms still conserve the nicosulfuron degradation capacity, using the same pathway as that observed with planktonic lifestyle as evidenced by LC-MS analyses. This pathway involved first the hydrolysis of the nicosulfuron sulfonylurea bridge, leading to the production of two major metabolites: 2-amino-4,6-dimethoxypyrimidine (ADMP) and 2-(aminosulfonyl)-N,N-dimethyl-3-pyridinecarboxamide (ASDM). One minor metabolite, identified as 2-(1-(4,6-dimethoxy-pyrimidin-2-yl)-ureido)-N,N-dimethyl-nicotinamide (N3), derived from the cleavage of the C-S bond of the sulfonylurea bridge and contraction by elimination of sulfur dioxide. A last metabolite (N4), detected in trace amount, was assigned to 2-(4,6-dimethoxy-pyrimidin-2-yl)-N,N-dimethyl-nicotinamide (N4), resulting from the hydrolysis of the N3 urea function. Although fungal growth was unaffected by nicosulfuron, its laccase activity was significantly impaired regardless of lifestyle. Leaf and wood surfaces being good substrata for biofilm development in rivers, P. cucumerina AR1 strain could thus have potential as an efficient candidate for the development of methods aiming to reduce contamination by nicosulfuron in aquatic environments.

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“…globosum is reported to be a fungal parasite which isolated from egg of Globodera pallida (cyst nematode) in USA. It showed the greatest reduction of the infection by G. pallida in potato of 76 % [29]. The endophytic Ch.…”

Section: Agricultural Inputsmentioning

confidence: 98%

Agricultural Inputs for Organic Agriculture

Soytong¹,

Song²,

Tongon³

2021

Proceedings of the International Seminar on Promoting Local Resources for Sustainable Agriculture and Development (ISPLRSAD 202

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Sustainable development concerns on saving energy and the environmental friendly reservation and to serve healthy and safety food. The history of agricultural production using chemical fertilizers, pesticides and other synthetic chemicals for crop and animal production is to increase yield become harzardous to human being and living things in our environments. We have proposed the modern organic agriculture model to serve the sustainable development goal (SDGs) that possible be a new combination practical technology for organic agriculture. The research investigation is still needed to find more bioproducts and natural producrts to contribute knowledge for non-agrochemical production (NAP) transition period to organic agricultural production. The demonstration organic model is proposed which consisted of organic crop production, organic animal production, understanding bioproducts and natural products as agricultural inputs, seed production, food preservation, animal feed, organically certification by trusted agencies, organic markets and practical training. The certified agricultural inputs for organic agriculture is needed for acceptable used eg. biological fertilizers (compost) and biofungicides, bioinsecticides, bioherbicides, and biological nutrition. The other combination techniques are proposed as cultural practice, crop rotation, inter-cropping, organic amendment, biochar technology. The net house, greenhouse in closed or semi-closed system to control temperature and relative humidity are also concerned. The herbs or medicinal plants, and natural wood vineagar to eliminate plant pathogens and insects are introduced. The light and yellow traps, natural pheromones, natural sulfur, white oil, repellent plants, neem-oil etc. are recommended. We have been conducted to investigate the bioproducts sine 1989 and those bioproducts are gradually contributed to farmers. Our research successfully findings are contributed as bio-remediation and bio-decomposer for revitalizing the soil to get rid of the heavy metals residue in the cultivated soil and make compost, the different formulations of organic-inorganic biological fertilizers with high nitrogen, phosphorous, potassium, calcium and magnesium etc. and biological nutrients for plant growth to be used instead of chemical fertilizers. Chaetomium myco-fungicide for broad spectrum plant disease control and the biodegradation natural product fine particles acts as natural elicitor for plant immunity. We have promoted to do organic agricultural production and certification until delivery to the markets.

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Prospecting fungal parasites of the potato cyst nematode Globodera pallida using a rapid screening technique

Cited by 13 publications

References 34 publications

Plants and Associated Soil Microbiota Cooperatively Suppress Plant-Parasitic Nematodes

Plants and Associated Soil Microbiota Cooperatively Suppress Plant-Parasitic Nematodes

Nicosulfuron Degradation by an Ascomycete Fungus Isolated From Submerged Alnus Leaf Litter

Agricultural Inputs for Organic Agriculture

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