Prospective Cohort Trial of<i>Euphrasia</i>Single-Dose Eye Drops in Conjunctivitis

Stoss, Matthias; Michels, Christoph; Peter, Ellen; Beutke, Ramona; Gorter, Robert W.

doi:10.1089/acm.2000.6.499

Cited by 36 publications

(22 citation statements)

References 14 publications

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“…Columns and bars show the mean±standard deviation (n=3). *p≤0.01 -culture of corneal cells treated with plant extracts compared to the non-treated culture control substances of the Euphrasia herb include iridoglicozoids (aucubin), tannins, phenolic acids, etheric oils, resin substances and flavonoids (2,5,18,19).…”

Section: Figmentioning

confidence: 99%

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Assessment of Eyebright (Euphrasia Officinalis L.) Extract Activity in Relation to Human Corneal Cells Using In Vitro Tests

Paduch¹,

Woźniak²,

Niedziela³

et al. 2014

Balkan Med J

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Herba Euphrasiae (Scrophulariaceae), commonly known as eyebright grass, is a composition of very similar plant species, broadly defined as Euphrasiae officinalis L., which are widely used in folk medicine and homeopathy (1, 2). E. officinalis L. is an annual, herbaceous, semi-parasitic plant which exhibits numerous biological activities, including anti-inflammatory, antioxidant, antimicrobial, anticancer, antifungal, antiviral, hypotensive, hapatoprotector, anti-epileptic, and anticatarrhal activities (3-8). In traditional medicine, E. officinalis L. has also been effectively used for the treatment of eye disorders like conjunctivitis, hordeolum, etc. (1, 5, 9). Eye drops made from E. officinalis L. are considered to be useful and safe in the treatment of ocular allergy symptoms (10). Folk medicine commonly uses eyebright aqueous extracts to treat eye disorders. Additionally, methanolic extracts of Euphrasia genus and its methanolic fractions have been evaluated for their antioxidant and anti-inflammatory activities (3). The biological activity of aqueous tinctures and alcohol extracts are based on the accumulation of highly active compounds like iridoids, flavonoids, phenolic acids or etheric oils (1, 2, 5). We may suppose that E. officinalis L. preparations for the treatment of eye disorders are generally based on their antioxidant, anti-inflammatory and regenerative properties. Moreover, as full extracts are used on the principle of the historical tradition, selective extracts are employed mainly for reason of their specific activity (11). Accordingly, in this study, we analyzed the cytotoxic, antioxidant and immunomodulatory effects of ethanol, ethyl acetate and heptane extracts of E. officinalis L. on human corneal epithelial (10.014 pRSV-T) cells.The aim of this study was to answer the question concerning the variety of biological effects of E. officinalis L. extracts obtained using different solvents by in vitro tests on human corneal cells, and their potential medicinal properties/effects when used in the treatment of eye disorders. MATERIAL AND METHODS Plant materialEuphrasia herbs were harvested from the area of Górki Czechowskie (Lublin, Poland) on a sunny day in September 2009. Background:Euphrasia officinalis L. is an herb traditionally used in folk medicine, mainly in the treatment of eye disorders. Aims: The present study analyzed the activity of three extracts of E. officinalis L. (ethanol, ethyl acetate and heptane) on cultured human corneal epithelial cells (10.014 pRSV-T). Study Design: In vitro study. Methods: Toxicity, free radical scavenging activity and the immunomodulatory effects of the extracts were tested using the thiazolyl blue tetrazolium bromide (MTT) or Neutral Red, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ELISA tests, respectively. Moreover, nitric oxide levels and cytoskeleton architecture were analyzed after corneal cell incubation with the plant extracts. Results: We show that the biological effect depended on both the concentration and the extraction solvent used. Heptane ext...

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Section: Figmentioning

confidence: 99%

“…Therefore, they may be potentially used in therapeutic supplementation, e.g. in ocular inflammation (2,5). It has been shown that flavonoids and polyphenols possess an inflammation reducing effect.…”

Section: Figmentioning

confidence: 99%

Assessment of Eyebright (Euphrasia Officinalis L.) Extract Activity in Relation to Human Corneal Cells Using In Vitro Tests

Paduch¹,

Woźniak²,

Niedziela³

et al. 2014

Balkan Med J

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“…A dosage of one drop three times a day seems to be the general prescribed dosage. It was published in the Journal of Alternative and Complementary Medicine in 2000 [5].…”

Section: Homeopathy and Viral Conjunctivitismentioning

confidence: 99%

Homeopathy and Epidemic Viral Conjunctivitis

Verma¹,

Verma²,

Atray³

et al. 2013

Homoeopathic Links

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“…• Eyebright (Euphrasia officinalis) eye drops have been found to be effective in a trial for various conjunctival conditions (Stoss et al 2000). makes a good wash for both eyes, using clean cotton wool for each eye.…”

Section: Herbal Treatment Of Childrenmentioning

confidence: 99%