Prospective Evaluation of the Chemiluminescence Test for the Detection of Granulocyte Antibodies: Comparison with the Granulocyte Immunofluorescence Test

Lucas, Geoff

doi:10.1159/000462494

Cited by 10 publications

(10 citation statements)

References 22 publications

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“…6 If specific neutrophil antigens are targeted, demonstration of immunoglobulin on neutrophils or their precursors can be used diagnostically, with a sensitivity ranging from 78% to 98%. 3,4,7 Targeting of specific antigen results in opsonization of neutrophils and removal by macrophages. The actual site of removal is presumably extravascular, within spleen or marrow or both.…”

Section: Discussionmentioning

confidence: 99%

Immune‐Mediated Neutropenia in 2 Dogs

McManus¹,

Litwin²,

Barber³

1999

Veterinary Internal Medicne

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Immune‐mediated neutropenia, also termed autoimmune neutropenia (AIN), is an uncommon event, rarely documented within veterinary literature, although the clinical profile and probable pathogenesis have been outlined in detail within medical literature. In this paper, we review 2 different manifestations of this disorder. The 1st case primarily involved peripheral destruction of mature neutrophils, with little impact on marrow precursors, whereas the 2nd case resulted in suppression of neutrophilic granulopoiesis within marrow. In both cases, absolute neutrophil counts dropped below 200/mL.

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Section: Discussionmentioning

confidence: 99%

Immune‐Mediated Neutropenia in 2 Dogs

McManus¹,

Litwin²,

Barber³

1999

Veterinary Internal Medicne

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“…As an alternative/addition to GAT, the GIFT/LIFT method may be complemented by the functional granulocyte chemiluminescence test (Hadley & Holburn, 1984; Lucas, 1994) where unfixed, heat‐treated granulocytes/lymphocytes (from the same donor) are incubated with patient sera. The incubated, unfixed cells are then added to a pool of random donor monocytes which phagocytose any granulocytes with bound antibody.…”

Section: Current Approaches To Hna Antibody Detectionmentioning

confidence: 99%

Human neutrophil antigens: Nature, clinical significance and detection

Browne

Dearman

Poles

2020

Int J Immunogenetics

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Within granulocyte immunology literature, the terms 'granulocyte' and 'neutrophil' are often used interchangeably. As neutrophils constitute >99% of the granulocyte population in homeostasis, the glycoproteins expressed by granulocytes are referred to collectively as the human neutrophil antigens (HNAs). This review will discuss the general characteristics of granulocytes and their contribution towards the most frequently associated clinical disorders/complications. An overview of the described HNA is provided with the most recent nomenclature as defined by the International Society of Blood Transfusion (ISBT). Current approaches to HNA characterization and antibody detection will also be outlined before finally discussing limitations and possible improvements. 1.1 | What are granulocytes? Granulocytes are granulocytic, polymorphonuclear white blood cells (WBC) produced in the bone marrow which are subdivided into neutrophils, basophils and eosinophils by the different staining properties of their granules. Neutrophils are the most abundant WBC (1.8-7.5 × 10 9 /L) in a healthy individual, whereas basophils and eosinophils are considerably less numerous (0.

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“…PMNs were separated from citrate-anticoagulated peripheral blood samples collected from several healthy individuals by the conventional method involving Ficoll density gradient centrifugation, harvesting of the polymorphonuclear cell layer and red cell lysis in NH 4 Cl solution (Lucas, 1994). Subsequently, the cells were purified by negative selection in a mixture of magnet-conjugated anti-CD3, anti-CD4, anti-CD8, anti-CD14, anti-CD19 and anti-CD56 Abs (Dynabeads, Dynal AS, Oslo, Norway), followed by harvesting of magnet-nonadherent cells as described previously (Ochensberger et al, 1999).…”

Section: Polymorphonuclear Neutrophil Preparationmentioning

confidence: 99%

Possible mechanisms underlying development of transfusion‐related acute lung injury: roles of anti‐major histocompatibility complex class II DR antibody

Nishimura

Mitsunaga

Ishikawa

et al. 2003

Transfusion Medicine

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Anti-major histocompatibility complex (anti-MHC) antibodies (Abs) and antipolymorphonuclear neutrophil (anti-PMN) Abs are generally considered as the main causes of the development of transfusion-related acute lung injury (TRALI), which is one of the most severe and sometimes lethal side effects of transfusion. These Abs are postulated to activate recipient's leucocytes, resulting in the release of soluble factors such as reactive oxygen species and detrimental cytokines and chemokines. The harmful effects on the lung tissues and resident leucocytes of these malignant factors are suspected to be profoundly involved in TRALI reactions. Several reports have indicated the principle effect of biologically active lipids on the pathogenesis of TRALI. However, the precise mechanisms of TRALI development remain unclear. To resolve this issue, we have been investigating cytokines that induce continuous inflammation of the lungs, specifically focusing on the cytokines derived from activated PMNs. We observed that the granulocyte-macrophage colony-stimulating factor (GM-CSF) markedly enhances the expression of MHC class II DR in PMNs. Moreover, MHC class II DR-expressing PMNs were also proved to express a high-affinity receptor for immunoglobulin E (IgE) (FcepsilonRI) and to produce tumour necrosis factor-alpha, interferon-gamma and interleukin-18 following a challenge with an anti-MHC class II DR monoclonal Ab (MoAb) or anti-DR antiserum. It is strongly suggested that amongst various inflammatory mediators, at least these three cytokines may contribute to the duration of inflammatory reactions in the lungs. Furthermore, FcepsilonRI expression, in GM-CSF-treated PMNs, suggests the involvement of PMNs in IgE-mediated immune reactions.

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Prospective Evaluation of the Chemiluminescence Test for the Detection of Granulocyte Antibodies: Comparison with the Granulocyte Immunofluorescence Test

Cited by 10 publications

References 22 publications

Immune‐Mediated Neutropenia in 2 Dogs

Immune‐Mediated Neutropenia in 2 Dogs

Human neutrophil antigens: Nature, clinical significance and detection

Possible mechanisms underlying development of transfusion‐related acute lung injury: roles of anti‐major histocompatibility complex class II DR antibody

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