“…Efforts to improve this process include various intraoperative techniques such as imprint cytology, 72 gross examination, 73 and ultrasound imaging. 74 However no extant method balances the accuracy, speed and ease of use required to achieve an ideal method of intraoperative analysis, and all present methods still result in 20-55% of removals requiring a secondary surgery. [75][76][77] The ability of SHG F/B to readily distinguish tumor from healthy tissue 61,63 coupled with the fact that it is a quantitative, intrinsic signal, suggests that it may provide useful information to assist with margin assessment.…”