Prospects for Plantation Growing Fast-Growing Triploid Clones of Aspen in the Southern Taiga Forest Area of the European Part of Russia

Bagaev, E. S.; Багаев, С С; Макаров, С.С.; Chudetsky, Anton

doi:10.21684/2411-7927-2018-4-3-81-93

Cited by 1 publication

(2 citation statements)

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“…Herewith, the maximum total length of triploid aspen roots (5.1 cm) was observed in the option with WPM and the IBA concentration of 1.0 mg/L (table 7). The results of previous experiments on replanting of adapted plants of triploid aspen with closed root system obtained by the in vitro method (without addition of growth-stimulating preparations) to the open ground [4] suggest the prospects of using the clonal micropropropagation method with the addition of modern growthstimulating preparations for accelerated production of planting material of triploid aspen forms for further plantation laying out.…”

Section: Research Results and Discussionmentioning

confidence: 98%

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Features of Triploid Aspen Clonal Micropropagation Using Modern Growth-Stimulating Preparations

Макаров¹,

Bagaev²,

Chudetsky³

et al. 2023

Lesnoy Zhurnal (Forestry Journal)

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The creation of fast-growing triploid aspen plantations in the area of wood processing enterprises has high importance in terms of increasing demand for deciduous wood associated with the development of board production and the prospects for the introduction of innovative technologies for deep processing of wood. The article presents the results of studies of triploid aspen clones of Kostroma origin at the stages of “introduction to in vitro culture”, “proper micropropagation”, and “rooting of microshoots” with the use of growth-regulating substances. At the “introduction culture to in vitro” stage, the most effective sterilizing agents were silver nitrate with the concentration of 0.2 %, Lysoformin 3000, 5 % applied for 15 min and sulema, 0.2 % used for 10 min. At the “proper micropropagation” stage, the total length of triploid aspen shoots did not differ significantly depending on the composition of the nutrient media studied but was slightly exceeded the options with Woody Plant Medium. An increase by 1.2–2.6 times was observed in the total length of triploid aspen microshoots in vitro, when the concentration of 6-benzylaminopurine in the nutrient medium was increased from 0.5 to 1.0 mg/L. The maximum value of the total length (5.6 cm) of tryploid aspen microshoots in vitro was observed on Woody Plant Medium at the 6-benzylaminopurine concentration of 1.0 mg/L and the presence of Epin-Extra preparation of 0.5 mg/L. At the “rooting of microshoots” stage the number, average, and total length of triploid aspen roots in vitro had no statistically significant differences depending on the nutrient medium composition. Increasing the concentrations of indole-3-butyric acid and indole-3-acetic acid from 1.0 to 2.0 mg/L in the nutrient medium increased the total length of regenerated plant roots of triploid aspen in vitro by 2.3–2.4 times. The maximum total length (5.1 cm) of triploid aspen roots in vitro was observed on Woody Plant Medium with indole-3-butyric acid at a concentration of 1.0 mg/L. Clonal micropropagation appears to be a perspective for accelerated production of elite planting material of triploid aspen for the purposes of laying out timber plantations. For citation: Makarov S.S., Bagaev E.S., Chudetsky A.I., Kuznetsova I.B., Lebedeva O.P., Antonov A.M. Features of Triploid Aspen Clonal Micropropagation Using Modern Growth-Stimulating Preparations. Lesnoy Zhurnal = Russian Forestry Journal, 2023, no. 2, pp. 183–194. (In Russ.). https://doi.org/10.37482/0536-1036-2023-2-183-194

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Section: Research Results and Discussionmentioning

confidence: 98%

“…It will eliminate the lack of small-sized wood raw materials for the development of pulp, board and biofuel industries [12]. Plantation forest growing enables to accelerate by 1.5-3 times the production of target wood compared to the traditional method [4]. The practice of the developed countries (Germany, Finland, Canada, Italy, etc.)…”

Section: Introductionmentioning

confidence: 99%