Prospects of Using Zein Markers in Breeding Waxy Maize Lines and Varieties

The effective use of maize (Zea mays L.) in agriculture depends entirely on the knowledge of its gene pool, which, in turn, needs to undergo registration and certification as new data become available. Maize is one of the model objects for applying the marker methodology. This article summarizes the results of a long-term study of the maize gene pool at N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) in two main areas: identification of the world genetic diversity of maize (including the most important wild relatives for breeding use), and search for reliable protein (zein) markers of traits valuable for breeding in order to use them in improvement, seed production, and seed control of this crop. Specific examples show the effectiveness of using zein markers in the development of new inbred lines and improvement of the existing ones. On the examples of such agronomic traits as early maturity, explosiveness, and waxiness of maize grain, the possibility of controlling the “inclusion” of these traits in the breeding material with the help of zein markers has been demonstrated. Evidence of the effectiveness of applying zein markers in the selection of parental pairs to obtain highly heterotic hybrids is presented. The nomenclature of zein adopted at VIR after its separation by polyacrylamide gel electrophoresis (PAGE) in an acidic buffer was compared with the nomenclature of zein after polyacrylamide gel electrophoresis with sodium dodecyl sulfate (SDS-PAGE) adopted among the foreign scientific community. The studies were performed on a large volume of accessions from the VIR collection as well as on breeding material provided by the country’s leading breeders.

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Correlation between quantitative traits and grain productivity of the waxy maize hybrids

Krivosheev

Ignatiev

Lupinoga

et al. 2023

Zernovoe hozâjstvo Rossii

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A subspecies of waxy maize (Zea mays L. ceratina) is characterized by the fact that its grain contains 100 % amylopectin starch, a valuable raw material for confectionery production, the manufacture of polymers, and a blood substitute. The absence of domestic waxy maize hybrids in the State List of Breeding Achievements necessitates the study in this area of breeding. The purpose of the current study was to identify dependencies that make it possible to increase the efficiency of the breeding process when developing waxy maize hybrids. The study was carried out at the FSBSI “ARC “Donskoy” in 2020–2022. The objects of the study were 36 waxy maize hybrids developed by the method of interline hybridization. The studied traits differed in the degree of variation. High coefficients of variation were identified in the traits ‘grain productivity’ (V = 30.1 %) and ‘one cob weight’ (V = 21.5 %). The traits ‘number of rows’ (V = 8.4 %) and ‘grain yield during threshing’ (V = 8.2 %) turned out to be slightly varying. An increase in the values of any studied trait resulted in an increase in grain yield. However, the signs of productivity were not equivalent according to their effect on the formation of the grain productivity of the waxy maize hybrids. There have been identified the most influential traits of productivity, such as ‘number of cobs per 1 plant’, ‘one cob weight’ and ‘number of seeds per cob’. The correlation between them and grain productivity is positive, close (r = 0.73±0.12 – 0.82±0.10). The identified traits have been positively associated with the rest (r = 0.30±0.16 – 0.74±0.12), the selection of samples with high values does not reduce the values of other productivity traits. The identified dependencies must be taken into account when selecting the initial material and when developing waxy maize hybrids.

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Prospects of Using Zein Markers in Breeding Waxy Maize Lines and Varieties

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Prof. Alexey V. Konarev (celebrating the 75th birthday)

Prof. Alexey V. Konarev (celebrating the 75th birthday)

Zein patterns as effective markers of valuable agronomic traits in maize

Correlation between quantitative traits and grain productivity of the waxy maize hybrids

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