Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines

Ramos‐Vega, Abel; Rosales‐Mendoza, Sergio; Bañuelos-Hernández, Bernardo; Angulo, Carlos

doi:10.3389/fmicb.2018.02506

Cited by 33 publications

(11 citation statements)

References 179 publications

(202 reference statements)

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“…Currently, yields in chloroplast expression are typically in the 0.02–5% TSP range with notable exceptions (42% of TSP of VP28) ( Surzycki et al, 2009 ). Few other algal species have shown yields up to 3 mg/l of bovine milk amyloid A protein by chloroplast expression and 15 mg/l of recombinant protein secretion in culture media by nuclear expression ( Lauersen et al, 2013 ; Gimpel et al, 2015 ; Ramos-Vega et al, 2018 ). Recombinant hemagglutinin proteins from the influenza virus have been expressed in the microalga Schizochytrium sp.…”

Section: Discussionmentioning

confidence: 99%

Efficient Transient Expression of Recombinant Proteins Using DNA Viral Vectors in Freshwater Microalgal Species

et al. 2021

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The increase in the world population, the advent of new infections and health issues, and the scarcity of natural biological products have spotlighted the importance of recombinant protein technology and its large-scale production in a cost-effective manner. Microalgae have become a significant promising platform with the potential to meet the increasing demand for recombinant proteins and other biologicals. Microalgae are safe organisms that can grow rapidly and are easily cultivated with basic nutrient requirements. Although continuous efforts have led to considerable progress in the algae genetic engineering field, there are still many hurdles to overcome before these microorganisms emerge as a mature expression system. Hence, there is a need to develop efficient expression approaches to exploit microalgae for the production of recombinant proteins at convenient yields. This study aimed to test the ability of the DNA geminiviral vector with Rep-mediated replication to transiently express recombinant proteins in the freshwater microalgal species Chlamydomonas reinhardtii and Chlorella vulgaris using Agrobacterium-mediated transformation. The SARS-CoV-2 receptor binding domain (RBD) and basic fibroblast growth factor (bFGF) are representative antigen proteins and growth factor proteins, respectively, that were subcloned in a geminiviral vector and were used for nuclear transformation to transiently express these proteins in C. reinhardtii and C. vulgaris. The results showed that the geminiviral vector allowed the expression of both recombinant proteins in both algal species, with yields at 48 h posttransformation of up to 1.14 μg/g RBD and 1.61 ng/g FGF in C. vulgaris and 1.61 μg/g RBD and 1.025 ng/g FGF in C. reinhardtii. Thus, this study provides a proof of concept for the use of DNA viral vectors for the simple, rapid, and efficient production of recombinant proteins that repress the difficulties faced in the genetic transformation of these unicellular green microalgae. This concept opens an avenue to explore and optimize green microalgae as an ideal economically valuable platform for the production of therapeutic and industrially relevant recombinant proteins in shorter time periods with significant yields.

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Section: Discussionmentioning

confidence: 99%

Efficient Transient Expression of Recombinant Proteins Using DNA Viral Vectors in Freshwater Microalgal Species

et al. 2021

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“…It should be mentioned here, that despite the high accumulation of PUFA in Schizochytrium spp. biomass, these heterotrophic species lack of chloroplasts [ 57 ]. Instead, the de novo FA synthesis has been evolutionarily shifted onto an ectoplasmic network [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Forage-to-Concentrate Ratio on Schizochytrium spp.-Supplemented Goats: Modifying Rumen Microbiota

Mavrommatis

Skliros

Sotirakoglou

et al. 2021

Animals

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The inclusion of feed additives and the implementation of various nutritional strategies are studied to modify the rumen microbiome and consequently its function. Nevertheless, rumen enzymatic activity and its intermediate products are not always matched with the microbiome structure. To further elucidate such differences a two-phase trial using twenty-two dairy goats was carried out. During the first phase, both groups (20HF n = 11; high forage and 20HG n = 11; high grain) were supplemented with 20 g Schizochytrium spp./goat/day. The 20HF group consumed a diet with a forage:concentrate (F:C) ratio of 60:40 and the 20HG-diet consisted of a F:C = 40:60. In the second phase, the supplementation level of Schizochytrium spp. was increased to 40 g/day/goat while the F:C ratio between the two groups were remained identical (40HF n = 11; high forage and 40HG n = 11; high grain). By utilizing a next-generation sequencing technology, we monitored that the high microalgae inclusion level and foremost in combination with a high grains diet increased the unmapped bacteria within the rumen. Bacteroidetes and Prevotella brevis were increased in the 40HG -fed goats as observed by using a qPCR platform. Additionally, methanogens and Methanomassiliicoccales were increased in high microalgae-fed goats, while Methanobrevibacter and Methanobacteriales were decreased. Fibrolytic bacteria were decreased in high microalgae-fed goats, while cellulolytic activity was increased. Ammonia was decreased in high grains-fed goats, while docosapentaenoic and docosahexaenoic acids showed a lower degradation rate in the rumen of high forage-fed goats. The alteration of the F:C ratio in goats supplemented with Schizochytrium spp. levels modified both ruminal microbiota and enzymatic activity. However, there was no significant consistency in the relations between them.

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“…In principle, these limitations could be overcome by bioengineering the parental bacterium to improve their OMV drugdelivery capability [60]. Alternatively, non-pathogenic [73][74][75] Modified from Corthesy et al [54]. VACCINES FOR EMERGING PATHOGENS: FROM RESEARCH TO THE CLINIC.…”

Section: Improving Mucosal Vaccinesmentioning

confidence: 99%

Mucosal vaccines and technology

Miquel-Clopés

Bentley

Stewart

et al. 2019

Clinical and Experimental Immunology

120

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Summary There is an urgent and unmet need to develop effective vaccines to reduce the global burden of infectious disease in both animals and humans, and in particular for the majority of pathogens that infect via mucosal sites. Here we summarise the impediments to developing mucosal vaccines and review the new and emerging technologies aimed at overcoming the lack of effective vaccine delivery systems that is the major obstacle to developing new mucosal vaccines.

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Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines

Cited by 33 publications

References 179 publications

Efficient Transient Expression of Recombinant Proteins Using DNA Viral Vectors in Freshwater Microalgal Species

Efficient Transient Expression of Recombinant Proteins Using DNA Viral Vectors in Freshwater Microalgal Species

The Effect of Forage-to-Concentrate Ratio on Schizochytrium spp.-Supplemented Goats: Modifying Rumen Microbiota

Mucosal vaccines and technology

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