Prostaglandin F2α stimulates angiogenesis at the embryo-maternal interface during early pregnancy in the pig

Kaczyński, Piotr; Goryszewska, Ewelina; Baryla, Monika; Wacławik, Agnieszka

doi:10.1016/j.theriogenology.2019.09.046

Cited by 25 publications

(19 citation statements)

References 40 publications

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“…In the present study, we demonstrated that the synergistic action of PGE2 with E2 in vivo increased the gene expression of SLCO2A1, which serves as a prostaglandin transporter. Our recent reports indicated a vital role for prostaglandins (PGE2 and PGF2α) in the establishment and development of pregnancy [1,22,[41][42][43][44]. The stimulating effect of E2 acting simultaneously with PGE2 on the expression of SLCO2A1 is the more interesting result, especially when we take into consideration our description of the role of E2 and PGE2 in the mechanism of the PGE2-positive feedback loop in the porcine endometrium [16].…”

Section: The Local Direct and Indirect Effects Of E2 And E2 Acting Together With Pge2 On The Endometrial Expression Of Genes Involved In mentioning

confidence: 94%

Synergistic action of estradiol and PGE2 on endometrial transcriptome in vivo resembles pregnancy effects better than estradiol alone†

Kaczyński

Bauersachs

Goryszewska

et al. 2020

Biology of Reproduction

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Successful pregnancy establishment in mammals depends on numerous interactions between embryos and the maternal organism. Estradiol-17β (E2) is the primary embryonic signal in the pig, and its importance has been questioned recently. However, E2 is not the only molecule of embryonic origin. In pigs, prostaglandin E2 (PGE2) is abundantly synthesized and secreted by conceptuses and endometrium. The present study aimed to determine the role of PGE2 and its simultaneous action with E2 in changes in porcine endometrial transcriptome during pregnancy establishment. The effects of PGE2 and PGE2 acting with E2 were studied using an in vivo model of intrauterine hormone infusions, and were compared to the effects of E2 alone and conceptuses’ presence on day 12 of pregnancy. The endometrial transcriptome was profiled using gene expression microarrays followed by statistical analyses. Downstream analyses were performed using bioinformatics tools. Differential expression of selected genes was verified by quantitative PCR. Microarray analysis revealed 2413 differentially expressed genes (DEGs) in the endometrium treated simultaneously with PGE2 and E2 (P < 0.01). No significant effect of PGE2 administered alone on endometrial transcriptome was detected. Gene ontology annotations enriched for DEGs were related to multiple processes such as: focal adhesion, vascularization, cell migration and proliferation, glucose metabolism, tissue remodeling, and activation of immune response. Simultaneous administration of E2 and PGE2 induced more changes within endometrial transcriptome characteristic to pregnancy than infusion of E2 alone. The present findings suggest that synergistic action of estradiol-17β and PGE2 resembles the effects of pregnancy on endometrial transcriptome better than E2 alone.

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Section: The Local Direct and Indirect Effects Of E2 And E2 Acting Together With Pge2 On The Endometrial Expression Of Genes Involved In mentioning

confidence: 94%

Synergistic action of estradiol and PGE2 on endometrial transcriptome in vivo resembles pregnancy effects better than estradiol alone†

Kaczyński

Bauersachs

Goryszewska

et al. 2020

Biology of Reproduction

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“…Three families of VEGF proteins and their corresponding receptors have been characterized and the main receptors involved in the first steps of signal transduction cascades comprise different tyrosine kinases receptors, such as VEGFR-1, VEGFR-2, and VEGFR-3 [ 42 ]. Across species, some VEGF family members and receptors are found in placentomes, uterus tissues, and oviduct, and in different species including humans, mice, rats, cattle, sheep, pigs, and rabbits [ 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ]. The in vivo administration of VEGF to goats and sheep stimulates follicular growth and increases the number of preovulatory follicles [ 51 ].…”

Section: Growth Factors and Early Developmentmentioning

confidence: 99%

“…FGF family members also play an important role in the early development of pig embryos. FGF-4 regulates TE formation and elongation [ 95 ], and the amount of FGF-2 increases in endometrial tissues between Days 15 and 20 of pregnancy, demonstrating its relevance in embryo elongation and implantation [ 45 ]. The mechanisms by which FGF family members regulate all these events, and which are the most relevant factors in each species, remain unknown.…”

Section: Growth Factors and Early Developmentmentioning

confidence: 99%

Pluripotency and Growth Factors in Early Embryonic Development of Mammals: A Comparative Approach

Llobat

2021

Veterinary Sciences

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The regulation of early events in mammalian embryonic development is a complex process. In the early stages, pluripotency, cellular differentiation, and growth should occur at specific times and these events are regulated by different genes that are expressed at specific times and locations. The genes related to pluripotency and cellular differentiation, and growth factors that determine successful embryonic development are different (or differentially expressed) among mammalian species. Some genes are fundamental for controlling pluripotency in some species but less fundamental in others, for example, Oct4 is particularly relevant in bovine early embryonic development, whereas Oct4 inhibition does not affect ovine early embryonic development. In addition, some mechanisms that regulate cellular differentiation do not seem to be clear or evolutionarily conserved. After cellular differentiation, growth factors are relevant in early development, and their effects also differ among species, for example, insulin-like growth factor improves the blastocyst development rate in some species but does not have the same effect in mice. Some growth factors influence genes related to pluripotency, and therefore, their role in early embryo development is not limited to cell growth but could also involve the earliest stages of development. In this review, we summarize the differences among mammalian species regarding the regulation of pluripotency, cellular differentiation, and growth factors in the early stages of embryonic development.

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“…Previously, PGF2α was reported as playing a luteolytic role through ROS-mediation mechanisms in luteal cells and corpus luteum of rats ( Tanaka et al., 2000 ; Riley and Behrman, 1991 ). The treatment of porcine trophoblast cells in vitro with luteolytic PGF2α promoted cell proliferation, adhesion and migration, and an increased expression of endometrial angiogenic factors ( Piotr et al., 2018 ; Kaczynski et al., 2020 ). This indicated a new role for PGF2α secreted by the conceptuses and endometrium in supporting pregnancy establishment during the implantation period.…”

Section: Activation Of Redox Pathways In Porcine Conceptus Trophectodermmentioning

confidence: 99%

Reactive oxygen and nitrogen species regulate porcine embryo development during pre-implantation period: A mini-review

Luo

Yao

2021

Animal Nutrition

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Significant porcine embryonic loss occurs during conceptus morphological elongation and attachment from d 10 to 20 of pregnancy, which directly decreases the reproductive efﬁciency of sows. A successful establishment of pregnancy mainly depends on the endometrium receptivity, embryo quality, and utero-placental microenvironment, which requires complex cross-talk between the conceptus and uterus. The understanding of the molecular mechanism regulating the uterine-conceptus communication during porcine conceptus elongation and attachment has developed in the past decades. Reactive oxygen and nitrogen species, which are intracellular reactive metabolites that regulate cell fate decisions and alter their biological functions, have recently reportedly been involved in porcine conceptus elongation and attachment. This mini-review will mainly focus on the recent researches about the role of reactive oxygen and nitrogen species in regulating porcine embryo development during the pre-implantation period.

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Prostaglandin F2α stimulates angiogenesis at the embryo-maternal interface during early pregnancy in the pig

Cited by 25 publications

References 40 publications

Synergistic action of estradiol and PGE2 on endometrial transcriptome in vivo resembles pregnancy effects better than estradiol alone†

Synergistic action of estradiol and PGE2 on endometrial transcriptome in vivo resembles pregnancy effects better than estradiol alone†

Pluripotency and Growth Factors in Early Embryonic Development of Mammals: A Comparative Approach

Reactive oxygen and nitrogen species regulate porcine embryo development during pre-implantation period: A mini-review

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