Prostaglandin release by cultured endometrial tissues after challenge with lipopolysaccharide and tumor necrosis factor α, in relation to the estrous cycle, treatment with medroxyprogesterone acetate, and pyometra

Jursza-Piotrowska, Ewelina; Socha, Piotr; Skarzynski, Dariusz J.; Siemieniuch, Marta

doi:10.1016/j.theriogenology.2015.11.034

Cited by 6 publications

(7 citation statements)

References 20 publications

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“…In cats, it is involved in the production of prostaglandins (PGs) during the estrous cycle [28,154]. The in vitro administration of TNFα in the endometrial tissue of estrus and diestrus cats increased the secretion of prostaglandin 2α (PGF 2α ) and prostaglandin E2 (PGE 2 ), respectively [154], which are similar effects to those found by Jursza et al [28] and Jursza-Piotrowska et al [155].…”

Section: Uterus and Placentasupporting

confidence: 73%

Molecular Factors Involved in the Reproductive Morphophysiology of Female Domestic Cat (Felis catus)

Santos,

Silva

2023

Animals

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The domestic cat (Felis catus) is considered an important model for the study of feline reproductive morphophysiology. However, although the morphological changes and clinical signs that occur during the estrous cycle and pregnancy are well known, little is known about the molecular mechanisms involved in the reproductive physiology of this animal species. Thus, this paper reviews the current knowledge about the modulation and expression profile of hormonal, immunological, redox, and growth mediators involved in the uterine, ovarian, and placental morphophysiology of domestic cats.

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Section: Uterus and Placentasupporting

confidence: 73%

Molecular Factors Involved in the Reproductive Morphophysiology of Female Domestic Cat (Felis catus)

Santos,

Silva

2023

Animals

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“…The interaction between toll-like receptors, found in the endometrium, and lipopolysaccharides found in the cell wall of bacteria, especially Escherichia coli, activate the inflammatory sequence. Then, this triggers the release of interleukin and tumor necrosis factor, important cytokines that also change the uterine blood flow (Jursza-Piotrowska et al, 2016). However, such information contrasts that reported in Singh et al (2018), in which the atrophic endometrium would be responsible for greater expression of inflammatory markers and hemodynamic changes.…”

Section: Resultsmentioning

confidence: 79%

Uterine artery hemodynamics in female dogs with open- and closed-cervix pyometra

Carvalho

Martins

Guimarães

et al. 2021

RSD

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Although pyometra is a common disease, the mechanisms that determine cervical opening remain unknown. Knowing that the vascular structures are crucial in pathophysiology, it was observed need for hemodynamic studies assessing uterine artery of female dogs with pyometra and its relation to the neck opening. Thirty-five female dogs were selected and separate into three groups: control group (CG) (n = 12), open-cervix pyometra group (OCG) (n = 11) and closed-cervix pyometra group (CCG) (n = 12), with the objective of evaluating and comparing the hemodynamic changes of the uterine artery [peak systolic velocity (PSV), end diastolic velocity (EDV), and resistance index (RI)] in female dogs with open- and closed-cervix pyometra and correlate them with measurements of uterine diameter (UD) and endometrial thickness (ET). The correlation analysis showed that, with the exception of PSV, the hemodynamics indices were associated with UD and ET, presenting a moderate and positive correlation between UD and EDV (r = 0.62; P<0.01), a moderate and negative correlation between UD and RI (r =-0.68; P<0.01) and also moderate and negative correlation between ET and RI (r = -0.62; P<0.01). These results suggest that alterations of uterine artery hemodynamics are similar in dog females with open- or closed-cervix pyometra, although the UD and the ET can influence in the uterine perfusion.

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“…Endometrial fragments were prepared as previously described [4, 6]. Using doses established in that study, cultured endometrial tissues were stimulated with 1 ng/ml human TNF-α (Sigma Aldrich, St. Louis, MO, USA), 50 ng/ml LPS purified from E. coli O55:B5 (Sigma Aldrich), and TNF-α with LPS (at the same concentrations).…”

Section: Methodsmentioning

confidence: 99%

“…Epithelial cells from cats at estrus (n = 5), and stromal cells from cats at diestrus (n = 5) were isolated from the endometrium according to a published protocol [6, 7]. After reaching 85% confluence, cells were stimulated at doses reported in a previous study [4] as follows: (i) 10 µl of sterile PBS served as a control, (ii) TNF-α (Sigma Aldrich) at 1 ng/ml, (iii) LPS purified from E. coli O55:B5 (Sigma Aldrich) at 50 ng/ml, and (iv) TNF-α together with LPS at the same concentrations.…”

Section: Methodsmentioning

confidence: 99%

“…In dogs, engagement of TLRs initiates a signaling cascade, resulting in altered expression of genes such as prostaglandin-endoperoxide synthase 2 ( PTGS2 ), PGE 2 -synthase (PGES), interleukin (IL)-1β , and tumor necrosis factor alpha ( TNF ) [5]. In cats, lipopolysaccharide (LPS), which is the main ligand for TLR4, is responsible for upregulation of PTGS2 , PGES , and prostaglandin F 2 α synthase ( PGFS ) mRNA in the cultured endometrial cells, and also for increasing prostaglandin secretion [6]. In an earlier study, we showed that treatment of cultured endometrial cells with LPS results in abundant TNF-α secretion; however, this effect was specific to epithelial cells (and not stromal cells) [7].…”

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confidence: 99%

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Comparison of the effect of lipopolysaccharide on tumor necrosis factor α (TNF-α) secretion and <i>TNF</i> and <i>TNFR1</i> mRNA levels in feline endometrium throughout the estrous cycle during pyometra and after medroxyprogesterone acetate treatment

Jursza-Piotrowska

Siemieniuch

2016

Journal of Reproduction and Development

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Endotoxins released by Gram-negative bacteria are potent stimulators of tumor necrosis factor α (TNF-α) production. The objectives of this study were to evaluate plasma levels of TNF-α, TNF-α secretion, and mRNA levels of TNF and TNF-α receptor type 1 (TNFR1) following exposure to lipopolysaccharide (LPS). For this, we used cultured endometrial cells or organ cultures, throughout the estrous cycle, after hormone treatment with medroxyprogesterone acetate (MPA), and during pyometra. Plasma TNF-α concentrations were increased in animals at estrus (P < 0.05) compared to other groups. In the LPS-challenged endometrium, secretion of TNF-α by tissues collected during estrus increased (P < 0.001) compared to that of other groups. LPS, alone or combined with TNF-α, upregulated TNF gene expression in the feline endometrium at diestrus (P < 0.001 for both treatments), in queens treated short-term with MPA (P < 0.01 and P < 0.05, respectively) and in queens treated long-term with MPA (P < 0.01 and P < 0.001, respectively). During pyometra, TNF and TNFR1 mRNA were increased only after tissues were challenged with TNF-α and LPS (P < 0.001 and P < 0.01, respectively). When cultured endometrial cells were challenged with LPS, the concentration of TNF-α increased only in epithelial cells after 4 h and 12 h (P < 0.05 and P < 0.01, respectively). Since LPS did not affect stromal cells, but TNF-α increased its own transcript after 2 h (P < 0.01), 4 h (P < 0.05) and 12 h (P < 0.001), we assume that stromal cells are not directly involved in pathogen recognition, as was the case for epithelial cells.

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Prostaglandin release by cultured endometrial tissues after challenge with lipopolysaccharide and tumor necrosis factor α, in relation to the estrous cycle, treatment with medroxyprogesterone acetate, and pyometra

Cited by 6 publications

References 20 publications

Molecular Factors Involved in the Reproductive Morphophysiology of Female Domestic Cat (Felis catus)

Molecular Factors Involved in the Reproductive Morphophysiology of Female Domestic Cat (Felis catus)

Uterine artery hemodynamics in female dogs with open- and closed-cervix pyometra

Comparison of the effect of lipopolysaccharide on tumor necrosis factor α (TNF-α) secretion and <i>TNF</i> and <i>TNFR1</i> mRNA levels in feline endometrium throughout the estrous cycle during pyometra and after medroxyprogesterone acetate treatment

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