Prosthecobacter fluviatilis sp. nov., which lacks the bacterial tubulin btubA and btubB genes

Abstract

Prosthecobacter fluviatilis sp. nov., which lacks the bacterial tubulin btubA and btubB genes Leptothrix cholodnii is a sheathed bacterium often found in metal-rich and oligotrophic aquatic environments. A bacterial strain that is able to degrade the NaOH-treated sheath of L. cholodnii was isolated. The isolate was a Gram-negative, aerobic and prosthecate bacterium. The optimum growth temperature and pH were 30 6C and pH 7.0, respectively. The DNA G+C content was 62.9 mol%. The major respiratory quinone was MK-6. A phylogenetic analysis based on the 16S rRNA gene indicated that the isolate is a member of the genus Prosthecobacter. The nearest relative was the type strain of Prosthecobacter vanneervenii, with a similarity of 97.1 %. However, the isolate does not possess the bacterial tubulin genes, btubA and btubB, unique to known species of the genus Prosthecobacter. It is proposed that the isolate represents a novel species, Prosthecobacter fluviatilis sp. nov. The type strain is HAQ-1

Microtubules in Bacteria: Ancient Tubulins Build a Five-Protofilament Homolog of the Eukaryotic Cytoskeleton

“…btubA and B genes are found in certain Prosthecobacter species including P. vanneervenii , P. dejongeii , and P. debontii , but not P. fluviatilis [11],[12],[24]. To begin, we verified that BtubA and BtubB proteins are in fact expressed in the species where the genes are present (Figures S1 and S2).…”

Pyrosequencing Analysis of Bacterial Diversity in 14 Wastewater Treatment Systems in China

“…Members of Zoogloea, such as Zoogloea ramigera, have long been considered the typical activated sludge bacteria responsible for the formation of activated sludge flocculation and to improve the purification process (11). Prosthecobacter are heterotrophic organisms which are often referred to as oligotrophic bacteria, meaning that they thrive in low-nutrient environments (25). This does not seem to apply to the Prosthecobacter organisms found here, as the influent biochemical oxygen demand (BOD) concentrations of the WWTPs were above 284.6 mg/liter.…”

Mercury and Other Heavy Metals Influence Bacterial Community Structure in Contaminated Tennessee Streams

“…Verrucomicrobia sequences have been recovered from numerous hydrocarbon-, mercury-, uranium-, and pesticide-contaminated environments, suggesting that they are resistant to a number of contaminants, possess metabolic plasticity, and may play a significant role in the decontamination process (14,57,66). Culturable representatives display diverse metabolic characteristics and range from aerobic heterotrophs involved in organic carbon transformations (41,67) to organisms thriving in metal-rich oligotrophic aquatic environments (78). While determining similar physiologies from phylogenetic data by using shorter reads is difficult, overall, 1,116 pyrosequences in this study clustered with known Verrucomicrobia species, using a cutoff of 0.15, with 750 sequences clustered with Opitutaceae sp.…”