Protaetia brevitarsis seulensis larvae ethanol extract inhibits RANKL-stimulated osteoclastogenesis and ameliorates bone loss in ovariectomized mice

Choi, Ra-Yeong; Kim, In-Woo; Ji, Moongi; Paik, Man‐Jeong; Ban, Eu-Jin; Lee, Joon Ha; Hwang, Jae Sam; Kweon, HaeYong; Seo, Minchul

doi:10.1016/j.biopha.2023.115112

Cited by 9 publications

(2 citation statements)

References 38 publications

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“…The most abundant fatty acid, oleic acid, accounts for more than 58% of the total fatty acids [ 9 , 11 ]. Although nutritional and functional chemical composition can be changed in response to feed materials and supplementary feeds [ 6 , 10 , 11 ], P. brevitarsis larvae also have a broad range of pharmacological activities, including anti-obesity and anti-diabetic effects [ 12 , 13 ], therapeutic effects on prostatic hyperplasia [ 14 ], the inhibition of osteoclastogenesis [ 15 ], neuroprotective effects against seizures and neurodegeneration [ 16 ], anti-bacterial and anti-inflammatory activities [ 17 , 18 ], and an anti-thrombotic function [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Hindgut Microbiota Variation in Protaetia brevitarsis Larvae across Diverse Farms

Min,

Cammayo-Fletcher

et al. 2024

Microorganisms

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Protaetia brevitarsis larvae are farm-raised for food, are used in traditional East Asian medicine, and convert organic waste into biofertilizers. Here, the comparative analysis of the gut microbiota of third-instar larvae obtained from five different farms was investigated using 16S rRNA microbial profiling. Species richness, evenness, and diversity results using α-diversity analysis (observed species, Chao1, Shannon, Simpson) were similar between farms, except for those between the TO and KO farms. β-diversity was significantly different in distribution and relative abundance between farms (PERMANOVA, pseudo-F = 13.20, p = 0.001). At the phylum level, Bacillota, Bacteroidota, Actinomycetota, and Pseudomonadota were the most dominant, accounting for 73–88% of the hindgut microbial community. At the genus level, Tuberibacillus, Proteiniphilum, Desulfovibrio, Luoshenia, and Thermoactinomyces were the most abundant. Although oak sawdust was the main feed component, there were large variations in distribution and relative abundance across farms at the phylum and genus levels. Venn diagram and linear discriminant analysis effect size analyses revealed large variations in the hindgut microbial communities of P. brevitarsis larvae between farms. These results suggest environmental factors were more important than feed ingredients or genetic predisposition for the establishment of the intestinal microbiota of P. brevitarsis larvae. These findings serve as reference data to understand the intestinal microbiota of P. brevitarsis larvae.

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Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Hindgut Microbiota Variation in Protaetia brevitarsis Larvae across Diverse Farms

Min,

Cammayo-Fletcher

et al. 2024

Microorganisms

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“…In addition, it suppressed reactive oxygen species generation and exhibited antioxidative effects [ 23 ]. A recent study reported that PB extracts regulated bone remodeling in mice with OVX-induced osteoporosis and decreased RANKL-stimulated osteoclastogenesis by inhibiting p38 MAPK activation and NF-κB phosphorylation [ 24 ]. Also, PB-derived proteins showed antioxidant properties and promoted osteogenic differentiation in human bone marrow-derived mesenchymal stem cells [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Protaetia brevitarsis Extract Attenuates RANKL-Induced Osteoclastogenesis by Inhibiting the JNK/NF-κB/PLCγ2 Signaling Pathway

Jang

Kim

et al. 2023

Nutrients

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Protaetia brevitarsis (PB)-derived bioactive substances have been used as food and medicine in many Asian countries because of their antioxidant, antidiabetic, anti-cancer, and hepatoprotective properties. However, the effect of PB extracts (PBE) on osteoclast differentiation is unclear. In this study, we investigated the effect of PBE on RANKL-induced osteoclastogenesis in mouse bone marrow-derived macrophages (BMMs). To investigate the cytotoxicity of PBE, the viability of BMMs was confirmed via MTT assay. Tartrate-resistant acid phosphatase (TRAP) staining and pit assays were performed to confirm the inhibitory effect of PBE on osteoclast differentiation and bone resorption. The expression levels of osteoclast differentiation-related genes and proteins were evaluated using quantitative real-time PCR and Western blotting. PBE attenuated osteoclastogenesis in BMMs in TRAP and pit assays without cytotoxicity. The expression levels of osteoclast marker genes and proteins induced by RANKL were decreased after PBE treatment. PBE suppressed osteoclastogenesis by inhibiting the RANKL-induced activated JNK/NF-κB/PLCγ2 signaling pathway and the expression of NFATc1 and c-Fos. Collectively, these results suggest that PBE could be a potential therapeutic strategy or functional product for osteoclast-related bone disease.

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Acidic polysaccharide from the edible insect Protaetia brevitarsis seulensis activates antiviral immunity to suppress norovirus infection

Olawuyi,

Heo,

Jeong

et al. 2025

Carbohydrate Polymers

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Protaetia brevitarsis seulensis larvae ethanol extract inhibits RANKL-stimulated osteoclastogenesis and ameliorates bone loss in ovariectomized mice

Cited by 9 publications

References 38 publications

Comparative Analysis of Hindgut Microbiota Variation in Protaetia brevitarsis Larvae across Diverse Farms

Comparative Analysis of Hindgut Microbiota Variation in Protaetia brevitarsis Larvae across Diverse Farms

Protaetia brevitarsis Extract Attenuates RANKL-Induced Osteoclastogenesis by Inhibiting the JNK/NF-κB/PLCγ2 Signaling Pathway

Acidic polysaccharide from the edible insect Protaetia brevitarsis seulensis activates antiviral immunity to suppress norovirus infection

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