Protaetiibacter larvae sp. nov. and Agromyces intestinalis sp. nov., isolated from the gut of larvae of Protaetia brevitarsis seulensis, reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb. nov. and emended description of the genus Pseudolysinimonas

Abstract

Two bacterial strains, FWR-8T and CFWR-9T, were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea. Both strains were strictly aerobic, Gram-stain-positive and non-motile. Strain FWR-8T possessed the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T and the phylogenetic tree revealed that strain FWR-8T formed a cluster with Ptb. intestinalis 2DFWR-13T. Pseudolysinimonas kribbensis MSL-13T and Lysinimonas yzui N7XX-4T shared a high 16S rRNA gene sequence similarity (97.8 %) and formed a cluster adjacent to the cluster that included Ptb. intestinalis 2DFWR-13T. The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces . The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively. Those from strain CFWR-9T were type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-11, MK-12 and MK-10, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively. Based on the phenotypic and genotypic data, strains FWR-8T and CFWR-9T each represent a novel species within the genera Protaetiibacter and Agromyces , respectively. For these species, the names Protaetiibacter larvae sp. nov. and Agromyces intestinalis sp. nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively. Our results also justify a reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb. nov. and an emended description of the genus Pseudolysinimonas isprovided.

Agromyces larvae sp. nov., a novel actinobacterium isolated from gut of larvae of Protaetia brevitarsis seulensis

“…The genome of strain CFWR-12 T has been found to harbour genes associated with virulence, resistance to antibiotics and multidrug resistance. Its nearest neighbour type strain and its closely related species were also been reported to contain genes coding for metal and antibiotic resistance as well as for beta-lactamase and multidrug resistance efflux pumps [15]. This is highly significant at this point as the source of strain CFWR-12 T in the current study and A.…”

“…Strain CFWR-12 T showed the highest sequence similarity to Agromyces intestinalis KACC 19306 T (99.0 %; NCBI Accession MT367292) and Agromyces protaetiae FW100M-8 T (97.9 %; NCBI Accession MK490987) both of which were previously isolated from the larval gut of PBS [4, 15]. Phylogenetic analysis showed that strain CFWR-12 T formed a robust clade with Agromyces intestinalis KACC 19306 T ().…”

“…One of the species, namely Agromyces mediolanus , has recently been reported to be infectious to humans [14]. In recent times, a few Agromyces species have been isolated from insect guts [4, 6, 15]. Insects, in general, have been historically documented to be consumed by humans and are currently considered as an efficient, economic and environmentally friendly source of nutrients [16].…”

Salinibacterium soli sp. nov., isolated from lakeside soil

“…The Gram-stain type was determined using the method described by Beveridge et al [20]. Growth at various temperatures was determined at 4-45 °C (4,10,15,20,25,28,30,35,37,40,42 and 45 °C) in R2A broth. Tolerance of NaCl was tested by growing the isolates in R2A broth supplemented with 0-5.0 % (w/v) NaCl at intervals of 0.5 %.…”

Agromyces cavernae sp. nov., a novel member of the genus Agromyces isolated from a karstic cave in Shaoguan

“…Species in the genus Agromyces exist in many habitats through the pedosphere, including forest soils, farm soils, plateau soils, stones and sea sediments [1,2,[6][7][8][9][10][11][12][13][14][15][16]. Additionally, some of the species of the genus Agromyces are found in symbiosis with other organisms, such as rhizospheres [17][18][19][20], gut of larva [21,22] or in the above-ground part of plants [23]. There are also reports on some species of the genus Agromyces taking part in plant xylems natural degradation [24] or sea food fermentation [25].…”