Protamine–Lipid–dsRNA Nanoparticles Improve RNAi Efficiency in the Fall Armyworm, <i>Spodoptera frugiperda</i>

Dhandapani, Ramesh Kumar; Gurusamy, Dhandapani; Palli, Subba Reddy

doi:10.1021/acs.jafc.2c00901

Cited by 29 publications

(16 citation statements)

References 64 publications

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“…In the case of the fall armyworm (Spodoptera frugiperda; Lepidoptera), the basic transcription levels of these core elements of RNAi machinery are similar to those observed in western corn rootworm (Diabrotica virgifera; Coleoptera) and southern green stink bug (Nezara viridula; Hemiptera) [39]. All three taxa are considered highly efficient species on which to employ RNAi strategies [40][41][42], from phylogenetic orders whose response to dsRNAi varies.…”

Section: Dsrna-mediated Silencing Core Machinerymentioning

confidence: 72%

Current Scenario of Exogenously Induced RNAi for Lepidopteran Agricultural Pest Control: From dsRNA Design to Topical Application

Lucena-Leandro

Abreu²,

Vidal³

et al. 2022

IJMS

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Invasive insects cost the global economy around USD 70 billion per year. Moreover, increasing agricultural insect pests raise concerns about global food security constraining and infestation rising after climate changes. Current agricultural pest management largely relies on plant breeding—with or without transgenes—and chemical pesticides. Both approaches face serious technological obsolescence in the field due to plant resistance breakdown or development of insecticide resistance. The need for new modes of action (MoA) for managing crop health is growing each year, driven by market demands to reduce economic losses and by consumer demand for phytosanitary measures. The disabling of pest genes through sequence-specific expression silencing is a promising tool in the development of environmentally-friendly and safe biopesticides. The specificity conferred by long dsRNA-base solutions helps minimize effects on off-target genes in the insect pest genome and the target gene in non-target organisms (NTOs). In this review, we summarize the status of gene silencing by RNA interference (RNAi) for agricultural control. More specifically, we focus on the engineering, development and application of gene silencing to control Lepidoptera through non-transforming dsRNA technologies. Despite some delivery and stability drawbacks of topical applications, we reviewed works showing convincing proof-of-concept results that point to innovative solutions. Considerations about the regulation of the ongoing research on dsRNA-based pesticides to produce commercialized products for exogenous application are discussed. Academic and industry initiatives have revealed a worthy effort to control Lepidoptera pests with this new mode of action, which provides more sustainable and reliable technologies for field management. New data on the genomics of this taxon may contribute to a future customized target gene portfolio. As a case study, we illustrate how dsRNA and associated methodologies could be applied to control an important lepidopteran coffee pest.

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Section: Dsrna-mediated Silencing Core Machinerymentioning

confidence: 72%

Current Scenario of Exogenously Induced RNAi for Lepidopteran Agricultural Pest Control: From dsRNA Design to Topical Application

Lucena-Leandro

Abreu²,

Vidal³

et al. 2022

IJMS

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“…Efforts have been made to increase the efficiency of RNAi, and a recent study indicated that chitosan nanoparticles and the Cellfectin II ( CFII ) transfection reagent helped dsRNA escape from endosomes and increased the RNAi response in S. frugiperda ( Ana et al, 2020 ; Gurusamy et al, 2020b ). The protamine-lipid-dsRNA nanoparticles, according to a more recent study by Dhandapani et al (2022) , increase RNAi efficiency in the target insect species S. frugiperda by shielding dsRNA from nuclease degradation and thereby significantly increasing the stability of dsRNA in the organism. Similarly, according to McGraw et al (2022) , clathrin-mediated endocytosis and macropinocytosis account for the majority of cellular uptake, and once inside the midgut, transcytosis is involved in moving branched amphipathic peptide nanocapsules (BAPCs) dsRNA from the lumen to the hemolymph of S. frugiperda .…”

Section: Methodsmentioning

confidence: 99%

RNA interference (RNAi) applications to the management of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae): Its current trends and future prospects

Kebede

Fite

2022

Front. Mol. Biosci.

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The fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) is among the invasive insect pests that damages maize and sorghum, the high-priority crops in newly colonized agro-ecologies, including African contexts. Owing to the increasing infestation of the pest and the limitations of current conventional methods for its management, there is a call for discovering advanced pest management approaches. RNA interference (RNAi) is an emerging molecular tool showing flexible potential for the management of S. frugiperda. We conducted a search of the recent application of RNAi literature using Google Scholar and Mendeley to find advanced papers on S. frugiperda management using RNAi molecular tools that led to growth inhibition, developmental aberrations, reduced fecundity, and mortality, mainly by disruption of normal biological processes of the pest. Although efforts have been made to accelerate the utility of RNAi, many factors limit the efficiency of RNAi to achieve successful control over S. frugiperda. Owing to RNAi’s potential bioactivity and economic and ecological acceptability, continued research efforts should focus on improving its broad applicability, including field conditions. Screening and identification of key target genes should be a priority task to achieve effective and sustainable management of this insect via RNAi. In addition, a clear understanding of the present status of RNAi utilization in S. frugiperda management is of paramount importance to improve its efficiency. Therefore, in this review, we highlight the biology of S. frugiperda and the RNAi mechanism as a foundation for the molecular management of the pest. Then, we discuss the current knowledge of the RNAi approach in S. frugiperda management and the factors affecting the efficiency of RNAi application. Finally, the prospects for RNAi-based insect pest management are highlighted for future research to achieve effective management of S. frugiperda.

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“…In the case of fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), the basic transcription rate of the core elements of RNAi machinery presents similar levels to those registered to western corn rootworm (Diabrotica virgifera virgifera) Coleoptera and southern green stink bug (Nezara viridula) Hemiptera [40]). All of them are high RNAi efficient species [41][42][43] although represent phylogenetic orders whose response to dsRNAi varies. The impact of dsRNA exposition via injection or feeding in modulation of core genes, remains unclear.…”

Section: Dsrna-mediated Silencing Machinerymentioning

confidence: 99%

Current Scenario of Exogenously Induced RNAi for Lepidopteran Agricultural Pest Control: From dsRNA Design to Topical Application

Lucena-Leandro¹,

Abreu²,

Vidal³

et al. 2022

Preprint

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Global crop yields are estimated to be reduced by 30–40% per year on account of plant pests and pathogens. Agricultural insect pests raise concerns about constraining global food security and climate changes contributing to the rise of infestation. The current management relies on plant breeding, associated or not with transgenes and chemical pesticides. Both approaches face serious technology obsolescence on the field due to resistance breakdown or development of insecticide resistance. The need for new Modes of Action (MoA) approaches in managing crop health grows each year, driven by market demands to reduce economic losses and phytosanitary requirements to meet the consumer perception. Disabling pest genes by sequence-specific expression silencing is considered a promising tool in the development of environment and health respectful biopesticides. The specificity conferred by long dsRNA-base solutions give support to minimizing effects on off-targeted genes in the insect pest genome and the target gene in non-target organisms (NTOs). In this review, we summarize the current status of gene silencing by RNA interference (RNAi) for agricultural control. More specifically, we focus on the engineering, development and application of gene silencing to control Lepidoptera by the employment of non-transforming dsRNA technologies. Despite some delivery and stability drawbacks of topical applications, we reviewed works showing convincing proof-of-concept results that point to imminent innovative solutions. Considerations about the regulamentation of the ongoing research on dsRNA-based pesticides to produce commercialized products for exogenous application are discussed. Academic and industry initiatives reveal a worthy effort to accomplish controlling Lepidoptera pests with this new mode of action to provide more sustainable and reliable technologies to field management. New data on genomics of this taxon encourage the increment of a customized target genes portfolio. As a case of study, we illustrate how dsRNA and associated methodologies could be applied to control an important Lepidopteran coffee pest.

show abstract

Protamine–Lipid–dsRNA Nanoparticles Improve RNAi Efficiency in the Fall Armyworm, Spodoptera frugiperda

Cited by 29 publications

References 64 publications

Current Scenario of Exogenously Induced RNAi for Lepidopteran Agricultural Pest Control: From dsRNA Design to Topical Application

Current Scenario of Exogenously Induced RNAi for Lepidopteran Agricultural Pest Control: From dsRNA Design to Topical Application

RNA interference (RNAi) applications to the management of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae): Its current trends and future prospects

Current Scenario of Exogenously Induced RNAi for Lepidopteran Agricultural Pest Control: From dsRNA Design to Topical Application

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