Protease detection in the biosensor era: A review

Rai, Pratika; Hoba, Sabrina N.; Buchmann, Celine; Subirana-Slotos, Robert J.; Kersten, Christian; Schirmeister, Tanja; Endres, Kristina; Bufe, Bernd; Tarasov, Alexey

doi:10.1016/j.bios.2023.115788

Cited by 2 publications

(2 citation statements)

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“…34 First, the complexity of biological samples may cause interference during the detection process or nonspecific interactions with the protease. 35 Second, considering that proteases are frequently found in samples at low concentrations and exhibit transient activity, the assay must be highly sensitive and responsive. 36 Additionally, the folded structure of proteins can limit access to the active site of the protease, requiring the introduction of signal amplification mechanisms to increase assay sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…These include the high complexity of the samples, the low concentration of the proteases, their transient active state, and the accessibility of the active site . First, the complexity of biological samples may cause interference during the detection process or nonspecific interactions with the protease . Second, considering that proteases are frequently found in samples at low concentrations and exhibit transient activity, the assay must be highly sensitive and responsive .…”

Section: Introductionmentioning

confidence: 99%

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Gold Nanocluster-Based Nanozyme for High-Throughput Colorimetric Detection of SARS-CoV-2

Ma,

Yin,

Wang

et al. 2024

ACS Appl. Nano Mater.

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There is an urgent need for efficient and convenient methods to detect SARS-CoV-2 as the global pandemic continues. Currently, detection of COVID-19 relies primarily on reverse transcription PCR (RT-PCR), a laboratory-based method that is time-consuming. To tackle this challenge, the study proposes an immediate high-throughput detection platform for SARS-CoV-2. The platform uses a gold nanoenzymatic colorimetric assay that focuses on the specific detection of 3CLpro in SARS-CoV-2. The platform has been extensively characterized for stability, sensitivity, detection range, and response time. The colorimetric detection method not only provides easy-to-read results but also allows color readings to be taken using a smartphone, with accuracy comparable to that of large instruments such as a microplate reader. The innovation of this technology lies in the ability to achieve instant high-throughput detection, which makes rapid detection of SARS-CoV-2 feasible. The proposed detection platform has a wide range of potential applications, particularly in the field of virus detection, providing an efficient and reliable solution for managing current pandemics and future disease prevention and control.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%