Protease from Mucor subtilissimus UCP 1262: Evaluation of several specific protease activities and purification of a fibrinolytic enzyme

Nascimento, Thiago Pajeú; Conniff, Amanda Sales; Moura, J.A.S.; Batista, Juanize Matias da Silva; Costa, Romero Marcos Pedrosa Brandão; Porto, Camila Souza; Campos-Takaki, Galba Maria de; Porto, Tatiana Souza

doi:10.1590/0001-3765202020200882

Cited by 11 publications

(2 citation statements)

References 41 publications

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“…It is well known that proteolytic enzymes of Aspergillus have a high potential for use as therapeutic agents as components of thrombolytic drugs and in the diagnosis of diseases of the hemostasis system as protein activators of proenzymes [ 33 , 34 , 35 ]. The obtained data allow us to judge the possibility of proteases of studied strains used as direct thrombolytics capable of cleaving blood clots in a few hours.…”

Section: Resultsmentioning

confidence: 99%

Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins

Osmolovskiy

Schmidt

Orekhova

et al. 2021

Life

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In this study, we investigated the properties of proteolytic enzymes of two species of Aspergillus, Aspergillus flavus 1 (with a high degree of pathogenicity) and Aspergillus ochraceus L-1 (a conditional pathogen), and their effects on various components of the hemostasis system (in vitro) in the case of their penetration into the bloodstream. We showed that micromycete proteases were highly active in cleaving both globular (albuminolysis) and fibrillar (fibrin) proteins, and, to varying degrees, they could coagulate the plasma of humans and animals (due to proteolysis of factors of the blood coagulation cascade) but were not able to coagulate fibrinogen. The proteases of both Aspergillus fully hydrolyzed thrombi in 120–180 min. Micromycetes did not show hemolytic activity but were able to break down hemoglobin.

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Section: Resultsmentioning

confidence: 99%

Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins

Osmolovskiy

Schmidt

Orekhova

et al. 2021

Life

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“…Some of the preparations of micromycete' proteinases have successfully passed preclinical and clinical trials [52,53]. Effective fibrin lysis was found in proteinases from Fusarium oxyporum [54], F. pallidoroseum [55], Paecilomyces tenuipes [56], Mucor subtilissimus [57], Rhizopus chinensis [58] and Rhizomucor miehei [59]. Proteases with plasminogen activating activity were found in representatives of micromycetes of different systematic and ecological-trophic groups: Arthrobotrys longa [60], Tolypocladium inflatum [61], Rhizopus microsporus var.…”

Section: Microbial Fibrinolytic Enzymes: Production Status and Diversitymentioning

confidence: 99%

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

2021

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Cardiac disorders such as acute myocardial infarction, embolism and stroke are primarily attributed to excessive fibrin accumulation in the blood vessels, usually consequential in thrombosis. Numerous methodologies including the use of anti-coagulants, anti-platelet drugs, surgical operations and fibrinolytic enzymes are employed for the dissolution of fibrin clots and hence ameliorate thrombosis. Microbial fibrinolytic enzymes have attracted much more attention in the management of cardiovascular disorders than typical anti-thrombotic strategies because of the undesirable after-effects and high expense of the latter. Fibrinolytic enzymes such as plasminogen activators and plasmin-like proteins hydrolyse thrombi with high efficacy with no significant after-effects and can be cost effectively produced on a large scale with a short generation time. However, the hunt for novel fibrinolytic enzymes necessitates complex purification stages, physiochemical and structural-functional attributes, which provide an insight into their mechanism of action. Besides, strain improvement and molecular technologies such as cloning, overexpression and the construction of genetically modified strains for the enhanced production of fibrinolytic enzymes significantly improve their thrombolytic potential. In addition, the unconventional applicability of some fibrinolytic enzymes paves their way for protein hydrolysis in addition to fibrin/thrombi, blood pressure regulation, anti-microbials, detergent additives for blood stain removal, preventing dental caries, anti-inflammatory and mucolytic expectorant agents. Therefore, this review article encompasses the production, biochemical/structure-function properties, thrombolytic potential and other surplus applications of microbial fibrinolytic enzymes.

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Mechanisms of Mucor sp. CM3 isolated from the aquatic macrophyte Eichhornia crassipes (Mart.) Solms to increase cadmium bioremediation

Almeida

Tschoeke

Quecine

et al. 2023

Environ Sci Pollut Res

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Protease from Mucor subtilissimus UCP 1262: Evaluation of several specific protease activities and purification of a fibrinolytic enzyme

Cited by 11 publications

References 41 publications

Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins

Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

Mechanisms of Mucor sp. CM3 isolated from the aquatic macrophyte Eichhornia crassipes (Mart.) Solms to increase cadmium bioremediation

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