Protease Inhibitors Prevent the Development of Human Rotavirus‐Induced Diarrhea in Suckling Mice

Ebina, Takusaburo; Tsukada, Keiko

doi:10.1111/j.1348-0421.1991.tb01589.x

Cited by 32 publications

(19 citation statements)

References 21 publications

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“…Thus these proteases constitute putative targets for chemotherapeutic inhibitors such as cystatin. Chicken cystatin has been described to reduce virus production of poliovirus-infected cells (Korant et al 1986) and is effective against human rotavirus infection in mice and consecutive diarrhea in suckling mice (Ebina and Tsukada 1991). Cystatin and cystatin-derived peptides display antibacterial activity against Porphyromonas gingivalis, frequently associated with periodontitis (Blankenvoorde et al 1998) and is also a potent inhibitor of cysteine proteases expressed by the Trypanosoma parasite responsible for bovine and human trypanosomiases (Serveau et al 1996).…”

Section: Proteinase Inhibitorsmentioning

confidence: 98%

Compounds with Antibacterial Activity

Baron

Réhault²

2007

Bioactive Egg Compounds

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Section: Proteinase Inhibitorsmentioning

confidence: 98%

Compounds with Antibacterial Activity

Baron

Réhault²

2007

Bioactive Egg Compounds

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“…ID 50 titre of the stock virus was estimated by the standard method [13]. A dose of 0.6 mg of TI/50 Ìl RV stock/g body weight was selected for further experiments after a pilot study (data not given) as used by Ebina and Tsukada [14]. Animals were divided into four groups as group 1 (healthy controls), group 2 (PEM), group 3 (PEM + RV) and group 4 (PEM + RV+ TI) [n = 36 each].…”

Section: Methodsmentioning

confidence: 99%

Influence of Soybean Trypsin Inhibitor on Small Bowel Enzyme Activities during Rotavirus Infection in Malnourished Infant Mice

Katyal¹,

Rana²,

Vaiphei

et al. 2000

Ann Nutr Metab

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Rotavirus (RV) is considered to be one of the major causes of acute episodic diarrhoea throughout the world. This study was undertaken to investigate the effect of soybean trypsin inhibitor (TI) on brush-border enzymes during rotavirus infection in protein energy malnourished (PEM) infant mice. Animals were divided into 4 groups, namely controls, PEM, PEM+RV and PEM+RV+TI (n = 36 each). Group 1 and 2 animals were orally inoculated with 50 µl of normal saline each. Group 3 animals were orally inoculated with 50 µl of 100 ID₅₀ dose of RV stock each. Group 4 animals were similarly inoculated with 0.6 mg TI/g body weight along with 50 µl of RV stock each. Animals were examined daily for diarrhoea and their body weight was recorded on alternate days postinoculation (dpi). Animals were killed by cervical dislocation after being given light chloroform anesthesia on 0, 1, 3, 5, 7 and 10 dpi. Small intestines were excised and homogenized in normal saline. Proteins, gammaglutamyl transpeptidase, alkaline phosphatase and disaccharidases were estimated in jejunum and ileum. Body weight was significantly reduced in PEM animals and with RV infection. Histologically, focal areas of vacuolar degeneration of lining epithelium were seen in RV-infected animals. Disaccharidases and other enzyme activities were decreased significantly in the PEM group compared to healthy controls and further depressed with RV infection in malnourished animals as compared to non-infected PEM. The enzyme activities were restored in animals receiving TI along with RV compared to the group receiving RV without TI. With the administration of soybean TI, the activities of disaccharidases, alkaline phosphatase, gammaglutamyl transpeptidase and intestinal architecture were restored showing a protective effect in PEM during RV infection.

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“…Diarrheal symptoms were lacking in most animals administered TI, indicating its protective efficacy. In an earlier study, histopathological changes in the small intestine reversed 2 days after RV infection with the administration of protease inhibitor [17]. Ileal epithelial cells were more involved in the infection, indicating the preferential tropism of small-intestinal regions.…”

Section: Incorporation Studies -Uptake Of [U-14 C]-l-leucinementioning

confidence: 99%

Protective Efficacy of Trypsin Inhibitor on the Gut following Rotavirus Infection in Malnourished Infant Mice

Katyal

Ojha²,

Rana³

et al. 1999

Ann Nutr Metab

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Epidemiologic data suggest a close relationship between protein energy malnutrition (or protein energy malnourished) (PEM) and development of infectious diarrheal diseases. In the present studies, the impact of trypsin inhibitor (TI) on the gut following rotavirus (RV) infection was investigated in PEM young growing inbred balb/c mice. The animals were divided into four groups: (1) control; (2) PEM; (3) PEM+RV and (4) PEM+ RV+TI. Control and PEM animals were orally inoculated with normal saline, PEM+RV animals were given 50 μl RV (100 ID₅₀) and PEM+RV+TI animals were inoculated with 0.6 mg TI/g body weight + 50 μl RV. The body weight of each animal was recorded. Six animals from each group were sacrificed on days 0, 1, 3, 5, 7 and 10 post inoculation by cervical dislocation. The intestines were removed, everted and homogenized in ice-cold saline. Leucine aminopeptidase (LAP), glutathione (GSH) levels and L-leucine uptake were determined in the jejunum and ileum. Body weight was decreased in PEM and PEM+RV groups compared to the controls. LAP and leucine uptake levels were elevated in the PEM group but decreased in the PEM+RV group. GSH levels were decreased in the PEM+RV group. Enzyme activity, GSH and LAP uptake levels were restored in the PEM+RV+TI group. The study shows the protective role of soybean TI against RV infection in malnourished mice.

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Protease Inhibitors Prevent the Development of Human Rotavirus‐Induced Diarrhea in Suckling Mice

Abstract: Oral inoculation of human rotavirus MO strain (serotype 3) into 5-day-

Cited by 32 publications

References 21 publications

Compounds with Antibacterial Activity

Compounds with Antibacterial Activity

Influence of Soybean Trypsin Inhibitor on Small Bowel Enzyme Activities during Rotavirus Infection in Malnourished Infant Mice

Protective Efficacy of Trypsin Inhibitor on the Gut following Rotavirus Infection in Malnourished Infant Mice

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