Protease sensing using nontoxic silicon quantum dots

Cheng, Xiaoyu; McVey, Benjamin F. P.; Robinson, Andrew B.; Longatte, Guillaume; O’Mara, Peter B.; Tan, Vincent T. G.; Thordarson, Pall; Tilley, Richard D.; Gaus, Katharina; Gooding, J. Justin

doi:10.1117/1.jbo.22.8.087002

Cited by 15 publications

(14 citation statements)

References 36 publications

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“…The cytotoxicity of SiQDs is also an important factor with regard to their final application in an organism [35,36]. The MTT method is the common way to assess the cytotoxicity of QDs.…”

Section: Resultsmentioning

confidence: 99%

One-Step Hydrothermal Synthesis of Yellow and Green Emitting Silicon Quantum Dots with Synergistic Effect

Zhang

Wei

et al. 2019

Nanomaterials

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The concept of synergistic effects has been widely applied in many scientific fields such as in biomedical science and material chemistry, and has further attracted interest in the fields of both synthesis and application of nanomaterials. In this paper, we report the synthesis of long-wavelength emitting silicon quantum dots based on a one-step hydrothermal route with catechol (CC) and sodium citrate (Na-citrate) as a reducing agent pair, and N-[3-(trimethoxysilyl)propyl]ethylenediamine (DAMO) as silicon source. By controlling the reaction time, yellow-emitting silicon quantum dots and green-emitting silicon quantum dots were synthesized with quantum yields (QYs) of 29.4% and 38.3% respectively. The as-prepared silicon quantum dots were characterized by fluorescence (PL) spectrum, UV–visible spectrum, high resolution transmission electron microscope (HRTEM), Fourier transform infrared (FT-IR) spectrometry energy dispersive spectroscopy (EDS), and Zeta potential. With the aid of these methods, this paper further discussed how the optical performance and surface characteristics of the prepared quantum dots (QDs) influence the fluorescence mechanism. Meanwhile, the cell toxicity of the silicon quantum dots was tested by the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium (MTT) bromide method, and its potential as a fluorescence ink explored. The silicon quantum dots exhibit a red-shift phenomenon in their fluorescence peak due to the participation of the carbonyl group during the synthesis. The high-efficiency and stable photoluminescence of the long-wavelength emitting silicon quantum dots prepared through a synergistic effect is of great value in their future application as novel optical materials in bioimaging, LED, and materials detection.

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“…The cytotoxicity of SiQDs is also an important factor with regard to their final application in an organism [35,36]. The MTT method is the common way to assess the cytotoxicity of QDs.…”

Section: Resultsmentioning

confidence: 99%

One-Step Hydrothermal Synthesis of Yellow and Green Emitting Silicon Quantum Dots with Synergistic Effect

Zhang

Wei

et al. 2019

Nanomaterials

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“…While these concepts can be employed for sensitive monitoring of clinically relevant proteases, they are very difficult to translate in modalities that would be applicable to clinical settings, mostly due to high costs and toxicity of materials for QD preparation. Fortunately, there are also non-toxic materials like silicon that can be used to make QDs [ 254 ], but even comparatively biocompatible QDs cannot not overcome the limitations of optical imaging.…”

Section: Monitoring Protease Activity In Cancermentioning

confidence: 99%

The Tumor Proteolytic Landscape: A Challenging Frontier in Cancer Diagnosis and Therapy

Vizovišek¹,

Ristanovic²,

Menghini³

et al. 2021

IJMS

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In recent decades, dysregulation of proteases and atypical proteolysis have become increasingly recognized as important hallmarks of cancer, driving community-wide efforts to explore the proteolytic landscape of oncologic disease. With more than 100 proteases currently associated with different aspects of cancer development and progression, there is a clear impetus to harness their potential in the context of oncology. Advances in the protease field have yielded technologies enabling sensitive protease detection in various settings, paving the way towards diagnostic profiling of disease-related protease activity patterns. Methods including activity-based probes and substrates, antibodies, and various nanosystems that generate reporter signals, i.e., for PET or MRI, after interaction with the target protease have shown potential for clinical translation. Nevertheless, these technologies are costly, not easily multiplexed, and require advanced imaging technologies. While the current clinical applications of protease-responsive technologies in oncologic settings are still limited, emerging technologies and protease sensors are poised to enable comprehensive exploration of the tumor proteolytic landscape as a diagnostic and therapeutic frontier. This review aims to give an overview of the most relevant classes of proteases as indicators for tumor diagnosis, current approaches to detect and monitor their activity in vivo, and associated therapeutic applications.

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“…The development of peptide-based assays to determine protease activity has advantages over conventional, non-specific protease assays using casein [ 63 ]. The success of the approach using magnetic nanoparticles [ 60 ], electrochemical sensing [ 64 , 65 ], calorimetry [ 66 ], mass spectrometry [ 67 ], quantum dots [ 68 ], and others was demonstrated for various proteases.…”

Section: Introductionmentioning

confidence: 99%

A Paper-Based Near-Infrared Optical Biosensor for Quantitative Detection of Protease Activity Using Peptide-Encapsulated SWCNTs

Shumeiko

Paltiel

Bisker

et al. 2020

Sensors

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A protease is an enzyme that catalyzes proteolysis of proteins into smaller polypeptides or single amino acids. As crucial elements in many biological processes, proteases have been shown to be informative biomarkers for several pathological conditions in humans, animals, and plants. Therefore, fast, reliable, and cost-effective protease biosensors suitable for point-of-care (POC) sensing may aid in diagnostics, treatment, and drug discovery for various diseases. This work presents an affordable and simple paper-based dipstick biosensor that utilizes peptide-encapsulated single-wall carbon nanotubes (SWCNTs) for protease detection. Upon enzymatic digestion of the peptide, a significant drop in the photoluminescence (PL) of the SWCNTs was detected. As the emitted PL is in the near-infrared region, the developed biosensor has a good signal to noise ratio in biological fluids. One of the diseases associated with abnormal protease activity is pancreatitis. In acute pancreatitis, trypsin concentration could reach up to 84 µg/mL in the urine. For proof of concept, we demonstrate the feasibility of the proposed biosensor for the detection of the abnormal levels of trypsin activity in urine samples.

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Protease sensing using nontoxic silicon quantum dots

Cited by 15 publications

References 36 publications

One-Step Hydrothermal Synthesis of Yellow and Green Emitting Silicon Quantum Dots with Synergistic Effect

One-Step Hydrothermal Synthesis of Yellow and Green Emitting Silicon Quantum Dots with Synergistic Effect

The Tumor Proteolytic Landscape: A Challenging Frontier in Cancer Diagnosis and Therapy

A Paper-Based Near-Infrared Optical Biosensor for Quantitative Detection of Protease Activity Using Peptide-Encapsulated SWCNTs

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