Protection against Brain histopathological damage in experimental Cerebral malaria models after exposure to hyperbaric oxigent

Utami, Prawesty Diah; Hadi, Usman; Dachlan, Yoes Prijatna; Suryokusumo, Guritno; Fitri, R. Loeki Enggar; Yudo, Varidianto

doi:10.52711/0974-360x.2021.00665

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…Prior to injection, the blood specimens were further diluted with normal saline. Finally, 0.2 mL of a 1 × 10 6 PbA-infected erythrocyte suspension was administered intraperitoneally to all the mice [28].…”

Section: Acclimatisation and Generation Of Pba Infected Mice Modelsmentioning

confidence: 99%

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2024

J. Med. Chem. Sci.

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Background: Malaria poses a substantial threat to healthcare systems across the globe. The escalating resistance of malaria parasites to the absence of affordable and accessible antimalarial medications has exacerbated the challenges associated with malaria treatment. This research aims to evaluate the antimalarial and antioxidant properties of Stichopus hermanni in combination with artemisinin, employing murine malaria models. Method: This study employed the 4-day suppression test procedure and conducted an experimental investigation with a post-test-only control group design. The study involved 35 male BALB/c mice, aged 7-10 weeks, each infected with Plasmodium berghei ANKA/PbA. The sample was divided into five groups: C1 (negative control), C2 (positive control), T1 (administered 15 mg/Kg BW/day of Stichopus hermanni ethanol extract and 0.04 mg/g BW/day of artemisinin), T2 (administered 17 mg/Kg BW/day of Stichopus hermanni ethanol extract and 0.04 mg/g BW/day of artemisinin), and T3 (administered 20 mg/Kg BW/day of Stichopus hermanni ethanol extract and 0.04 mg/g BW/day of artemisinin). The parameters measured included % parasitemia level, % growth inhibition, and malondialdehyde (MDA) levels. Results: The T3 group exhibited the lowest parasitemia level and the highest degree of growth inhibition compared to the other groups (p-value < 0.05). However, statistically, it did not differ significantly from C2 and T1. Meanwhile, the T3 group demonstrated the lowest MDA levels and the most significant antioxidant capacity compared to the other groups (pvalue < 0.05). Conclusion: Based on both descriptive and statistical analyses, the combination of Stichopus hermanni ethanol extract and artemisinin exhibits antimalarial and antioxidant activities.

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Section: Acclimatisation and Generation Of Pba Infected Mice Modelsmentioning

confidence: 99%

Untitled

2024

J. Med. Chem. Sci.

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“…Severe malaria has been observed to induce multiorgan failure in adults, whereas children commonly experience severe anemia, respiratory distress, or cerebral malaria. Infection with several Plasmodium species other than P. falciparum can lead to substantial morbidity and, in some cases, pose a risk to human life [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

In Silico Approach Triterpene Glycoside of H. atra Targeting Orotidine 5-Monophosphate Decarboxylase Protein (PfOMPDC) in P. falciparum Infection Mechanism

Utami,

Setianingsih,

Sari

2024

BioMed Research International

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This study accessed the potential antimalarial activity of triterpene glycoside of H. atra through targeting orotidine 5-monophosphate decarboxylase protein (PfOMPDC) in P. falciparum by molecular docking. Nine triterpene glycosides from H. atra extract modeled the structure by the Corina web server and interacted with PfOMPDC protein by using Hex 8.0.0. The docking results were visualized and analyzed by Discovery Studio version 21.1.1. 17-Hydroxyfuscocineroside B showed the lowest binding energy in PfOMPDC interaction, which was -1,098.13 kJ/mol. Holothurin A3, echinoside A, and fuscocineroside C showed low binding energy. Nine triterpene glycosides of H. atra performed interaction with PfOMPDC protein at the same region. Holothurin A1 posed interaction with PfOMPDC protein by 8 hydrogen bonds, 3 hydrophobic interactions, and 8 unfavorable bonds. Several residues were detected in the same active sites of other triterpene glycosides. Residue TYR111 was identified in all triterpene glycoside complexes, except holothurin A3 and calcigeroside B. In summary, the triterpene glycoside of H. atra is potentially a drug candidate for malaria therapeutic agents. In vitro and in vivo studies were required for further investigation.

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Comparison between the Diagnostic Performances of Rapid Diagnostic Test (RDT) using Advantage Malaria Card Pf/Pv Ag, Microscopy, and Polymerase Chain Reaction (PCR) in Malaria Suspected patients at the Merauke Regional General Hospital

Betaubun

Adiatmaja

Butarbutar

et al. 2023

RJPT

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Malaria prevalence in Indonesia is still high, especially in eastern Indonesia, namely Papua, reaching a peak of 16%, for example, in Merauke. The diagnosis of Malaria is conducted according to the gold standard, using the microscopy method. However, it is still limited, raising the need for a Rapid Diagnostic Test (RDT) examination. This study aims to evaluate the diagnostic performances of Immunochromatography (ICT) based RDT (using Advantage Malaria Card Pf/Pv Ag), compared to the diagnostic performances of microscopy and Polymerase Chain Reaction (PCR).This cross-sectional observational study took all fever patients of outpatient and inpatient care at the Merauke Regional General Hospital (hereinafter referred to as RSUD Merauke) from June to July 2019 as the samples. The sample population included all malaria patients with positive microscopy results, and the control population included all non-malarial fever patients at RSUD Merauke. Each specimen underwent microscopy (thick and thin preparations), RDT, and Real-Time (RT)-PCR tests using a Rotor Gene-Q (Qiagen) with abTESTM malaria 5qPCR III reagent. The diagnostic performances of RDT were analyzed by calculating its sensitivity, specificity, Positive Predictive Value (PPV), and Negative Predictive Value (NPV). The study was conducted on 105 samples, namely 55 malaria samples and 50 control samples, who underwent microscopy, RDT, and RT-PCR. RDT sensitivity to microscopy, P. vivax 100%, P. falciparum 89.5%; specificity of P. vivax 95.7%, P. falciparum 97.7%. RDT sensitivity to RT-PCR, P. vivax 53.5%, P. falciparum 48.6%; specificity of P. vivax 100%, P. falciparum 98.5%. The RDT p-value for microscopy and RT-PCR was p=0.000. RDT k-coefficient of microscopy, P. vivax 0.937, P. falciparum 0.871. RDT k-coefficient of PCR, P. vivax 0.427, P. falciparum 0.531. RDT is more sensitive to P. vivax than microscopy. RDT is more specific to P. vivax than RT-PCR. Further studies are suggested to discuss anti-malaria drug resistance and sequencing.

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Protection against Brain histopathological damage in experimental Cerebral malaria models after exposure to hyperbaric oxigent

Cited by 4 publications

References 23 publications

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In Silico Approach Triterpene Glycoside of H. atra Targeting Orotidine 5-Monophosphate Decarboxylase Protein (PfOMPDC) in P. falciparum Infection Mechanism

Comparison between the Diagnostic Performances of Rapid Diagnostic Test (RDT) using Advantage Malaria Card Pf/Pv Ag, Microscopy, and Polymerase Chain Reaction (PCR) in Malaria Suspected patients at the Merauke Regional General Hospital

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